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Evaluating the Potential of an Antibody Against Recombinant OmpW Antigen in Detection of Vibrio cholerae by Surface Plasmon Resonance (SPR) Biosensor

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Abstract

Surface plasmon resonance (SPR), a highly sensitive and label-free optical biosensing technique, is a powerful tool for studying biomolecular interactions. An immunosensor for rapid, sensitive, and selective detection of Vibrio cholerae on the basis of SPR is reported. Recombinant OmpW antigen (a bacterial outer-membrane protein) of V. cholerae was expressed and purified and raising of polyclonal rabbit anti-OmpW was done. Antibodies were immobilized on a sensor surface and interactions between OmpW protein and the whole cell of V. cholerae with immobilized antibodies were studied in different experiments. The aim of this study was to evaluate the potential of anti-OmpW in detection of V. cholerae by developing an immunosensor based on SPR. The results showed high affinity interaction between OmpW and anti-OmpW (K D = 2.4 ± 0.07 × 10−9 M) and SPR signals had a linear relationship with the number of V. cholerae ranging from 1 × 102 to 1 × 107 cells/mL with limit of detection of 50 cells/mL. The specificity of the developed immunoassay was examined using some non-V. cholerae bacteria which did not produce any significant responses. This method is rapid, sensitive, and specific to target V. cholerae with a total analysis time of less than 60 min.

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Abbreviations

SPR:

Surface plasmon resonance

SAM:

Self-assembled monolayer

11 MUA:

11-mercaptoundecanoic acid

OMP:

Outer membrane proteins

V. cholerae :

Vibrio cholerae

WHO:

World health organization

IPTG:

Isopropyl-b-d-thio-galactopyranoside

SDS PAGE:

Sodium dodecylsulphate polyacrylamide gel electrophoresis

Ni-NTA:

Ni-Nitrilotriacetic acid

EDC:

N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride

NHS:

N-hydroxysuccinimide

ATR–FTIR:

Attenuated total reflection fourier transform infrared

pI:

Isoelectric point

K D :

Dissociation constant

B max :

Maximum binding capacity of analyte

CFU:

Colony forming unit

ELISA:

Enzyme-linked immunosorbent assay

LOD:

Limit of detection

IMS:

Immunomagnetic separation

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Acknowledgments

This paper has been extracted from Ph.D thesis. The authors would like to acknowledge all the supports of University of Tehran and the financial support of Baqiyatallah University of Medical Sciences (Grant No. 91-1127). The authors also wish to thank Dr. Jan. Castrop, Kinetic Evaluation BV, The Netherlands, for his kind consulting and updating our “kinetic evaluation” software.

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Authors and Affiliations

  1. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran

    Ramezan Ali Taheri, Ali Hossein Rezayan, Fereshteh Rahimi & Javad Mohammadnejad

  2. Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Mehdi Kamali

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  1. Ramezan Ali Taheri
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  2. Ali Hossein Rezayan
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Correspondence to Ali Hossein Rezayan.

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Taheri, R.A., Rezayan, A.H., Rahimi, F. et al. Evaluating the Potential of an Antibody Against Recombinant OmpW Antigen in Detection of Vibrio cholerae by Surface Plasmon Resonance (SPR) Biosensor. Plasmonics 12, 1493–1504 (2017). https://doi.org/10.1007/s11468-016-0411-2

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