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IP-10 and fractalkine induce cytotoxic phenotype of murine NK cells

  • Article
  • Life & Medical Sciences
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Science Bulletin

Abstract

We characterized the murine NK cell subsets of the tumor microenvironment (TME) with low expressions of CD16 and NKG2D and investigated the chemokines that deter CD16low NKG2Dlow subsets. Our results demonstrated the activation of primary and KY-1 NK cell by ligands and found that exogenous CXCL10/interferon-gamma-induced protein 10 (IP-10) and fractalkine (FKN) can up-regulate the expression of CD16 and NKG2D. Moreover, both IP-10 and FKN are shown to facilitate migration, adhesion and cytotoxicity of NK cell subsets of the TME, due to the up-regulated CD16 and NKG2D. Overall, our data provide a new path by which to enhance murine NK cell cytotoxic potential and improve the quality of NK cells of the TME.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (81171975) and the Tianjin Institutes for Basic Sciences (15JCYBJC26900). We would like to thank Mr. Jie Zhang for his assistance in flow cytometric analysis.

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    Authors and Affiliations

    1. Key Lab of Animal Models and Neurodegenerative Diseases, Medical School of Nankai University, Tianjin, 300071, China

      Fang Liu, Junfang Qin, Hongyao Zhang, Ning Li, Meihua Shan & Yue Wang

    2. State Key Laboratory of Medical Chemical Biology of Nankai University, Tianjin, 300071, China

      Junfang Qin

    3. Tianjin Cancer Hospital of Tianjin Medical University, Tianjin, 300060, China

      Lan Lan

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    1. Fang Liu
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    2. Junfang Qin
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    7. Yue Wang
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    Correspondence to Yue Wang.

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    Fang Liu and Junfang Qin contributed equally to this work.

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    Liu, F., Qin, J., Zhang, H. et al. IP-10 and fractalkine induce cytotoxic phenotype of murine NK cells. Sci. Bull. 61, 202–211 (2016). https://doi.org/10.1007/s11434-015-0961-2

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    • DOI: https://doi.org/10.1007/s11434-015-0961-2

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