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Integrated solid-state nanopore devices for third generation DNA sequencing

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Abstract

Third generation DNA sequencing relies on monitoring the ionic current blockage during the DNA molecule’s threading through a nanoscale pore. It is still really tough to attain the single base discrimination on a DNA strand by merely analyzing the ionic current due to speedy DNA translocation and low spatial resolution. More integrated configurations are pursued to present versatile comparative dissimilarities of the four bases by enhancing the spatial resolution within a DNA molecule translocation event, such as transverse tunneling current, local potential change, and capacitance oriented voltage resonance. In this mini review, the insight is provided into the status quo on several functionalized techniques and methodologies for DNA sequencing and furthermore concluding remark and outlook are presented.

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  1. Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing, 211189, China

    GenSheng Wu, Yin Zhang, Wei Si, JingJie Sha, Lei Liu & YunFei Chen

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  1. GenSheng Wu
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  2. Yin Zhang
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  3. Wei Si
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Correspondence to YunFei Chen.

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Wu, G., Zhang, Y., Si, W. et al. Integrated solid-state nanopore devices for third generation DNA sequencing. Sci. China Technol. Sci. 57, 1925–1935 (2014). https://doi.org/10.1007/s11431-014-5644-8

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