Skip to main content
Log in

Shortened snRNA promoters for efficient CRISPR/Cas-based multiplex genome editing in monocot plants

  • Letter to Editor
  • Published:
Science China Life Sciences Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Čermák, T., Curtin, S.J., Gil-Humanes, J., Čegan, R., Kono, T.J.Y., Konečná, E., Belanto, J.J., Starker, C.G., Mathre, J.W., Greenstein, R. L., et al. (2017). A multipurpose toolkit to enable advanced genome engineering in plants. Plant Cell 29, 1196–1217.

    Article  Google Scholar 

  • Cong, L., Ran, F.A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P.D., Wu, X., Jiang, W., Marraffini, L.A., et al. (2013). Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819–823.

    Article  CAS  Google Scholar 

  • Connelly, S., Marshallsay, C., Leader, D., Brown, J.W., and Filipowicz, W. (1994). Small nuclear RNA genes transcribed by either RNA polymerase II or RNA polymerase III in monocot plants share three promoter elements and use a strategy to regulate gene expression different from that used by their dicot plant counterparts. Mol Cell Biol 14, 5910–5919.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ding, D., Chen, K., Chen, Y., Li, H., and Xie, K. (2018). Engineering introns to express RNA guides for Cas9- and Cpf1-mediated multiplex genome editing. Mol Plant 11, 542–552.

    Article  CAS  Google Scholar 

  • Engler, C., Kandzia, R., and Marillonnet, S. (2008). A one pot, one step, precision cloning method with high throughput capability. PLoS ONE 3, e3647.

    Article  Google Scholar 

  • Li, G., Liu, Y.G., and Chen, Y. (2019). Genome-editing technologies: the gap between application and policy. Sci China Life Sci 62, 1534–1538.

    Article  Google Scholar 

  • Liu, W., Xie, X., Ma, X., Li, J., Chen, J., and Liu, Y.G. (2015). DSDecode: A web-based tool for decoding of sequencing chromatograms for genotyping of targeted mutations. Mol Plant 8, 1431–1433.

    Article  CAS  Google Scholar 

  • Ma, X., Zhang, Q., Zhu, Q., Liu, W., Chen, Y., Qiu, R., Wang, B., Yang, Z., Li, H., Lin, Y., et al. (2015). A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol Plant 8, 1274–1284.

    Article  CAS  Google Scholar 

  • Xie, K., Minkenberg, B., and Yang, Y. (2015). Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Proc Natl Acad Sci USA 112, 3570–3575.

    Article  CAS  Google Scholar 

  • Xie, X., Ma, X., Zhu, Q., Zeng, D., Li, G., and Liu, Y.G. (2017). CRISPR-GE: A convenient software toolkit for CRISPR-based genome editing. Mol Plant 10, 1246–1249.

    Article  CAS  Google Scholar 

  • Zeng D., Ma, X., Xie, X., Zhu, Q., and Liu, YG. (2018). A protocol for CRISPR/Cas9-based multi-gene editing and sequence decoding of mutant sites in plants (in Chinese). Sci Sin Vitae 48, 783–794.

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (91435203 and 31991222) and the Major Program of Guangdong Basic and Applied Basic Research (2019B030302006).

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Jingxin Guo or Yao-Guang Liu.

Ethics declarations

Compliance and ethics The author(s) declare that they have no conflict of interest.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hao, Y., Zong, W., Zeng, D. et al. Shortened snRNA promoters for efficient CRISPR/Cas-based multiplex genome editing in monocot plants. Sci. China Life Sci. 63, 933–935 (2020). https://doi.org/10.1007/s11427-019-1612-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11427-019-1612-6

Navigation