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Characterization of transgenic mice expressing EGFP under the control of the monkey 20α-hydroxysteroid dehydrogenase promoter

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Abstract

To directly assess the molecular function of the monkey 20α-hydroxysteroid dehydrogenase (20α-HSD) promoter, we generated transgenic mice (tg) expressing enhanced green fluorescent protein (EGFP) under control of this promoter. We demonstrated that prostaglandin F2α induced 20α-HSD promoter activity in CHO cells in a dose-dependent manner. Furthermore, forskolin treatment markedly reduced 20α-HSD promoter activity, and prolactin exhibited weak inhibitory activity. The transgenic mouse obtained one positive founder male. The transgene was propagated in 10 successive generations without any notable defects to the progeny. EGFP and 20α-HSD in the tg mice were colocalized in the luteal cells of the ovary during late pregnancy. Strong EGFP and 20α-HSD protein signals were also detected in the adult testis. Immunohistochemical analysis revealed high EGFP levels in the seminiferous epithelium, whereas 20α-HSD was expressed in the seminiferous tubules. Our data suggest that the ovaries in monkey and mouse exhibit similar expression patterns of 20α-HSD during pregnancy. However, the expression pattern of EGFP in tg mice testis slightly differed from that of the endogenous 20α-HSD. Further investigation is required to elucidate the functional mechanisms underlying regulation of the monkey 20α-HSD promoter in the tg mice.

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Acknowledgements

The authors thank Dr. HW Seong (Institute of Animal Science) for his helpful discussions. This study was supported by the KRIBB Research Initiative Program (KGM4251622), Republic of Korea.

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Authors and Affiliations

  1. Animal Biotechnology, Graduate School of Future Convergence Technology, Department of Animal Life Science, Institute of Genetic Engineering, Hankyong National University, Ansung, 17579, Republic of Korea

    Chae-Won Park, Seong-Keun Jeong, Tsevelmaa Nanjidsuren, Munkhzaya Byambaragchaa & Kwan-Sik Min

  2. Department of Food Science and Nutrition, Hoseo University, Asan, 31499, Republic of Korea

    Myung-Hwa Kang

  3. National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang, 28116, Republic of Korea

    Bo-Woong Sim

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  1. Chae-Won Park
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Park, CW., Jeong, SK., Nanjidsuren, T. et al. Characterization of transgenic mice expressing EGFP under the control of the monkey 20α-hydroxysteroid dehydrogenase promoter. Sci. China Life Sci. 61, 966–974 (2018). https://doi.org/10.1007/s11427-017-9111-9

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  • DOI: https://doi.org/10.1007/s11427-017-9111-9

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