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Crosstalk-free colloidosomes for high throughput single-molecule protein analysis

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Abstract

Droplet microfluidics is a powerful platform for high-throughput single-molecule protein analysis. However, the issues of coalescence and crosstalk of droplets compromise the accuracy of detection and hinder its wide application. To address these limitations, a novel colloidosome-based method was presented by combining a Pickering emulsion with droplet microfluidics for single-molecule protein analysis. Utilizing the self-assembly of easily synthesized colloidal surfactant F-SiO2 NPs at the water/oil interface, the colloidosomes are rigidly stabilized and can effectively avoid the leakage of fluorescent molecules. The crosstalk-free colloidosomes enable high-throughput single-molecule protein analysis, including heterogenous dynamic studies and digital detection. As a robust and accurate method, colloidosome-based microfluidics is promising as a powerful tool for a wide variety of applications, such as directed enzyme evolution, digital enzyme-linked immunosorbent assay (ELISA), and screening of antibiotics.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (21775128, 21974113, 21927806, 21735004, 21435004, 21521004), the Program for Chang Jiang Scholars and Innovative Research Teams in University (IRT13036) and the National Science Fund for Fostering Talents in Basic Science (NFFTBS, J1310024).

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Authors and Affiliations

  1. The Key Laboratory of Chemical Biology of Fujian Province, the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Engineering, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China

    Kun Yin, Xi Zeng, Xuan Liang, Haopai Wei, Huimin Zeng, Wei Qi, Weidong Ruan, Yanling Song, Chaoyong Yang & Zhi Zhu

  2. Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China

    Chaoyong Yang

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  1. Kun Yin
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Correspondence to Zhi Zhu.

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Yin, K., Zeng, X., Liang, X. et al. Crosstalk-free colloidosomes for high throughput single-molecule protein analysis. Sci. China Chem. 63, 1507–1514 (2020). https://doi.org/10.1007/s11426-020-9818-9

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  • DOI: https://doi.org/10.1007/s11426-020-9818-9

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