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Anethole and eugenol reduce in vitro and in vivo leukocyte migration induced by fMLP, LTB4, and carrageenan

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Abstract

The aim of this study was to investigate the effect of anethole (AN) and eugenol (EUG) on leukocyte migration using in vitro chemotaxis and in situ microcirculation assays. BALB/c mice were used for the in vitro chemotaxis assay, and Wistar rats for the in situ microcirculation assay. We evaluated (a) the in vitro leukocyte migration in response to chemotactic factors (formyl-methionyl-leucyl-phenylalanine [fMLP] and leukotriene B4 [LTB4]) and (b) the rolling, adhesion, and migration of leukocytes induced by an injection of carrageenan (100 µg/cavity) into the scrotum of the animal. In the in vitro chemotaxis assay, AN and EUG at doses of 1, 3, 9, and 27 µg/ml significantly inhibited leukocyte migration when stimulated by the chemotactic agents fMLP and LTB4. In the in situ microcirculation assay, AN at doses of 125 and 250 mg/kg and EUG at a dose of 250 mg/kg significantly decreased the number of leukocytes that rolled, adhered, and migrated to perivascular tissue. The results indicate that AN and EUG exert inhibitory effects on leukocyte migration, highlighting their possible use to diminish excessive leukocyte migration in the inflammatory process.

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Acknowledgments

We thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and the Fundação Araucária (FA) for their financial support, and Mr. Jailson Araujo Dantas for his technical assistance.

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The authors declare that there are no potential conflicts of interest regarding the work submitted for publication.

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Authors and Affiliations

  1. Laboratory of Inflammation, Department of Pharmacology and Therapeutic, State University of Maringá, Maringá, PR, Brazil

    Camila Fernanda Estevão-Silva, Raquel Kummer, Fernanda Carolina Fachini-Queiroz, Renata Grespan, Gessilda Alcântara Nogueira de Melo, Silmara Baroni, Roberto Kenji Nakamura Cuman & Ciomar Aparecida Bersani-Amado

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  1. Camila Fernanda Estevão-Silva
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  2. Raquel Kummer
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  3. Fernanda Carolina Fachini-Queiroz
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  4. Renata Grespan
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Correspondence to Ciomar Aparecida Bersani-Amado.

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Estevão-Silva, C.F., Kummer, R., Fachini-Queiroz, F.C. et al. Anethole and eugenol reduce in vitro and in vivo leukocyte migration induced by fMLP, LTB4, and carrageenan. J Nat Med 68, 567–575 (2014). https://doi.org/10.1007/s11418-014-0839-7

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