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Phosphorylation-dependent proteome of Marcks in ependyma during aging and behavioral homeostasis in the mouse forebrain

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Abstract

Ependymal cells (ECs) line the ventricular surfaces of the mammalian central nervous system (CNS) and their development is indispensable to structural integrity and functions of the CNS. We previously reported that EC-specific genetic deletion of the myristoylated alanine-rich protein kinase C substrate (Marcks) disrupts barrier functions and elevates oxidative stress and lipid droplet accumulation in ECs causing precocious cellular aging. However, little is known regarding the mechanisms that mediate these changes in ECs. To gain insight into Marcks-mediated mechanisms, we performed mass spectrometric analyses on Marcks-associated proteins in young and aged ECs in the mouse forebrain using an integrated approach. Network analysis on annotated proteins revealed that the identified Marcks-associated complexes are in part involved in protein transport mechanisms in young ECs. In fact, we found perturbed intracellular vesicular trafficking in cultured ECs with selective deletion of Marcks (Marcks-cKO mice), or upon pharmacological alteration to phosphorylation status of Marcks. In comparison, Marcks-associated protein complexes in aged ECs appear to be involved in regulation of lipid metabolism and responses to oxidative stress. Confirming this, we found elevated signatures of inflammation in the cerebral cortices and the hippocampi of young Marcks-cKO mice. Interestingly, behavioral testing using a water maze task indicated that spatial learning and memory is diminished in young Marcks-cKO mice similar to aged wildtype mice. Taken together, our study provides first line of evidence for potential mechanisms that may mediate differential Marcks functions in young and old ECs, and their effect on forebrain homeostasis during aging.

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Data availability

Proteomics data that support the findings of this study are openly available in PanoramaWeb at https://panoramaweb.org, reference number 20210423. Additional data that support the findings of this study are available from the corresponding author upon request.

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Acknowledgements

This work is supported by NIH grant R01NS098370 to HTG. Drs. Laura Sommerville and Jennifer Parker provided technical support.

Funding

national institute of neurological disorders and stroke,R01NS098370,H. Troy Ghashghaei

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Authors and Affiliations

  1. Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA

    Nagendran Muthusamy, Kenneth B. Adler & H. Troy Ghashghaei

  2. Molecular Education, Technology and Research Innovation Centre (METRIC), North Carolina State University, Raleigh, NC, USA

    Taufika I. Williams & David C. Muddiman

  3. Department of Chemistry, North Carolina State University, Raleigh, NC, USA

    Taufika I. Williams & David C. Muddiman

  4. Pediatrics and Rare Diseases Group, Sanford Research, Sioux Falls, SD, USA

    Ryan O’Toole, Jon J. Brudvig & Jill M. Weimer

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  1. Nagendran Muthusamy
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Contributions

H.T.G. and N.M. conceived the project, analyzed data, and wrote the manuscript. N.M. performed experiments; T.I.W. and D.C.M. conducted mass spectrometry, nanoLC-MS/MS analysis, and helped edit the manuscript; J.J.B. and J.M.W. validated proteomic data and helped edited the manuscript; K.B.A provided reagents.

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Correspondence to H. Troy Ghashghaei.

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KA holds 130,000 founders’ shares of a start-up biotech company, Biomarck, and serves as a scientific consultant without monetary compensation. KA receives over $100,000 yearly in research grants from the National Institutes of Health (NIH). The remaining authors have declared no competing interests.

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Muthusamy, N., Williams, T.I., O’Toole, R. et al. Phosphorylation-dependent proteome of Marcks in ependyma during aging and behavioral homeostasis in the mouse forebrain. GeroScience 44, 2077–2094 (2022). https://doi.org/10.1007/s11357-022-00517-3

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