Abstract
The increasing use of polypropylene (PP) in consumer products leads to the microplastic (PP MPs) contamination of the aquatic ecosystems. Comprehensive toxicological studies of weathered/aged and new PP MPs with Artemia salina are a need of the hour. Our study explores the toxicological differences between naturally weathered (aged) and prepared new PP MPs on Artemia salina. Both the weathered and new PP MPs were prepared using controlled grinding and sieving at ≤ 125 µm. Artemia salina was treated with different concentrations (0.25, 0.5, and 1 mg/mL) of PP MP particles for up to 48 h. The uptake of weathered PP MP particles by Artemia salina was higher than the new PP MPs. The accumulation of PP MP particles was found in the intestine. There was increased oxidative stress recorded in the animal treated with the weathered PP MPs than the new PP MPs. Artemia salina treated with weathered PP MPs showed higher ROS generation and increased, activity of oxidative enzymes like LPO, SOD, and CAT. Collectively, our findings underscore the detrimental effects of weathered and prepared new PP MPs on Artemia salina, which is an ecologically significant species of zooplankton. There is an urgent need and effective measures required to address plastic disposal strategies in an environmentally safe manner.
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Data availability
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- PP MPs:
-
Polypropylene microplastics
- NSW:
-
Natural sea water
- ROS:
-
Reactive oxygen species
- LPO:
-
Lipid peroxidation
- SOD:
-
Superoxide dismutase
- CAT:
-
Catalase
- TBA:
-
Thiobarbituric acid
- TCA:
-
Trichloroacetic acid
- MDA:
-
Malondialdehyde
- DCFH-DA:
-
2′,7′-Dichlorofluorescin diacetate
- Na2CO3 :
-
Sodium carbonate
- NBT:
-
Nitroblue tetrazolium chloride
- H2O2 :
-
Hydrogen peroxide
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The authors express their gratitude to the Indian Council of Medical Research (ICMR) for providing financial support through Grant-F⋅No. 36/2/2020/Toxi/ BMS.
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SK: methodology, investigation, formal analysis, data curation, validation; RS: resources; AM: project administration, funding acquisition; NC: conceptualization, idea behind the whole manuscript, drafting, review and editing, methodology, supervision.
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Kanimozhi, S., Seenivasan, R., Mukherjee, A. et al. Naturally weathered polypropylene microplastic from environment and its toxic behaviour in Artemia salina. Environ Sci Pollut Res 31, 13207–13217 (2024). https://doi.org/10.1007/s11356-024-31998-8
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DOI: https://doi.org/10.1007/s11356-024-31998-8