Abstract
In this study, a laboratory-scale partial nitrification reactor (PN reactor) was used to treat high-ammonia–nitrogen wastewater, by changing the influent NH4+-N conversion rate as the main operating strategy, to investigate the upper limit of its NH4+-N conversion rate (ACR) and explore its feasibility as an anammox pre-process. During the experiment, PN reactor was successfully activated in only 10 days. The PN reactor reached the highest ACR value of approximately 10.24 kg N/(m3 · day) when the influent ACR was 16.57 kg N/(m3 · day), and the ammonia conversion efficiency (ACE) was 61.78% at this time. The ratio of [NO2−-N]Eff/[NH4+-N]Eff was approximately 1.37 which was close to the theoretical ratio of 1.32. And feasibility exploration experiment proved that it was feasible to use this PN reactor as a pre-process of anammox. The PCR-DGGE results showed that the dominant phylum and genus in the reactor during the ACR experiment were Proteobacteria and Nitrosomonas, respectively. With the increase in the ACR, the relative concentration of Nitrosomonas sp. G1 increased from 15 to 40%. This indicates that its abundance is directly correlated with the increase in the ACR. High-throughput sequencing showed that increasing the ACR of the PN reactor greatly reduced the diversity and abundance of the system microbial community structure and changed the dominant phylum and genus; however, the stability of the system was not disrupted. High-throughput sequencing experiments showed that the abundance value of nitrosation enzymes accounted for 91.62%, which was positively correlated with the expression of nitrification genes in the genus Nitrosomonas.
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References
Anthonisen AC, Loehr RC, Prakasam TBS (1976) Inhibition of nitrification by free ammonia and nitrous acid. J Water Pollut Control Fed 48(5):835–52. https://www.jstor.org/stable/25038971
Busse HJ, Kampfer P, Moore ERB, Nuutinen J, Tsitko IV, Denner EBM, Vauterin L, Valens M, Rossello-Mora R, Salkinoja-Salonen MS (2002) Thermomonas haemolytica gen. nov., sp. nov., a gamma-proteobacterium from kaolin slurry. Int J Syst Evol Microbiol 52:473. https://doi.org/10.1099/00207713-52-2-473
Chaparro JM, Badri DV, Vivanco JM (2014) Rhizosphere microbiome assemblage is affected by plant development. ISME J Multidiscip J Microb Ecol 8(4):790. https://doi.org/10.1038/ismej.2013.196
Chen J, Zeng J, He YR, Sun SQ, Wu HP, Zhou YY, Chen ZG, Wang JH, Chen H (2023b) Insights into a novel nitrogen removal process based on simultaneous anammox and denitrification (SAD) following nitritation with in-situ NOB elimination. J Environ Sci 125:160–170. https://doi.org/10.1016/j.jes.2022.01.019
Chen H, Tu Z, Wu S, Yu GL, Du CY, Wang H, Yang EZ, Zhou L, Deng B,Wang DB, Li HL (2021) Recent advances in partial denitrification-anaerobic ammonium oxidation process for mainstream municipal wastewater treatment. Chemosphere 278. https://doi.org/10.1016/j.chemosphere.2021.130436
Chen H, Liu K, Yang EZ, Chen J, Gu YL, Wu S, Yang M, Wang H, Wang DB, Li HL (2023a) A critical review on microbial ecology in the novel biological nitrogen removal process: dynamic balance of complex functional microbes for nitrogen removal. Sci Total Environ 857. https://doi.org/10.1016/j.scitotenv.2022.159462
Cheng J, Zhang L, Zhang S et al (2017) Effect of ammonia nitrogen load fluctuation on nitrate nitrogen in partial nitrification of urban sewage-anammox process. CHN Environ Sci 37(02):520–525. https://doi.org/10.3969/j.issn.1000-6923.2017.02.015
Chi Y, Zhang X, Xi X, Ren T, Jin P (2021) Quick start-up of partial nitrification in a novel anaerobic/ pulse washout (APW) process for treating municipal wastewater. J Cleaner Production 285. https://doi.org/10.1016/j.jclepro.2020.124850
Chung J, Shim H, Park SJ, Kim SJ, Bae W (2006) Optimization of free ammonia concentration for nitrite accumulation in shortcut biological nitrogen removal process. Bioprocess Biosyst Eng 28:275–282. https://doi.org/10.1007/s00449-005-0035-y
Collins MD, Lund BM, Farrow JAE et al (1983) Chemotaxonomic study of an alkalophilic bacterium, Exiguobacterium aurantiacum gen. nov. sp nov. Microbiology 129(7):2037–42. https://doi.org/10.1099/00221287-129-7-2037
Gao JL, Wang R, Li YL, Huang HM, Su XM, An ZJ, Yin WJ, Yang LZ, Rong L, Sun FQ (2022) Effect of aeration modes on nitrogen removal and N2O emission in the partial nitrification and denitrification process for landfill leachate treatment. Sci Total Environ 853. https://doi.org/10.1016/j.scitotenv.2022.158424
He YL, Tao WD, Wang ZY, Shayya W (2012) Effects of pH and seasonal temperature variation on simultaneous partial nitrification and anammox in free-water surface wetlands. J Environ Manage 110:103–109. https://doi.org/10.1016/j.jenvman.2012.06.009
Huang XW, Urata K, Wei QY, Yamashita Y, Hama T, Kawagoshi Y (2016) Fast start-up of partial nitritation as pre-treatment for anammox in membrane bioreactor. Biochem Eng J 105:371–378. https://doi.org/10.1016/j.bej.2015.10.018
Ibrahim M, Yusof N, Yusoff MZM, Hassan MA (2016) Enrichment of anaerobic ammonium oxidation (anammox) bacteria for short start-up of the anammox process: a review. Desalination Water Treat 57(30):13958–13978. https://doi.org/10.1080/19443994.2015.1063009
Ivanova N, Rohde C, Munk C (2011) Complete genome sequence of Truepera radiovictrix type strain (RQ-24T). Stand Genom Sci 4(1):91–99. https://doi.org/10.4056/sigs.1563919
Jetten MSM, Wagner M, Fuerst J, van Loosdrecht M, Kuenen G, Strous M (2001) Microbiology and application of the anaerobic ammonium oxidation (‘anammox’) process. Curr Opin Biotechnol 12(3):283–288. https://doi.org/10.1016/S0958-1669(00)00211-1
Jin Y, Wang DQ, Zhang WJ (2015) Use of bamboo charcoal reduced the cultivated anammox seed sludge dosage during the start-up period. Desalination Water Treat 57(43):20248–20253. https://doi.org/10.1016/j.biortech.2019.122101
Johansen JR, Mares J, Pietrasiak N, Bohunicka M, Zima J, Stenclova L, Hauer T (2017) Highly divergent 16S rRNA sequences in ribosomal operons of Scytonema hyalinum (Cyanobacteria). Plos One 12(10):e0186393. https://doi.org/10.1371/journal.pone.0186393
Kuypers MMM, Marchant HK, Kartal B (2018) The microbial nitrogen-cycling network. Nat Rev Microbiol 16(5):263–276. https://doi.org/10.1038/nrmicro.2018.9
Lackner S, Gilbert EM, Vlaeminck SE, Joss A, Horn H, van Loosdrecht MCM (2014) Full-scale partial nitritation/anammox experiences — an application survey. Water Res 55:292–303. https://doi.org/10.1016/j.watres.2014.02.032
Lei L, LI F, LU L, et al (2017) Curvibacter sp. strain HJ-1 induced the formation of aragonite under the condition of low Mg/Ca ratio . Acta Microbiologica Sinica 57(3): 434–46. https://europepmc.org/article/med/29756597
Li BJ, Wang Y, Guo Y, Wang WH, Huang XZ, Wang Z (2021) Partial nitrification coupled with anammox in a biofilter reactor (BR) of large height-to-diameter ratio for treatment of wastewater with low C/N. Process Biochem 104:19–26. https://doi.org/10.1016/j.procbio.2021.02.015
Li YL, Zhang WJ, Dai Y, Su XM, Xiao YY, Wu D, Sun FQ, Mei RW, Chen JR, Lin HJ (2022) Effective partial denitrification of biological effluent of landfill leachate for Anammox process: start-up, influencing factors and stable operation. Sci Total Environ 807. https://doi.org/10.1016/j.scitotenv.2021.150975
Liu XN, Wang HQ, Li HX, Jin Y, Zhang WJ (2019a) Carbon sequestration pathway of inorganic carbon in partial nitrification sludge. Bioresour Technol 293. https://doi.org/10.1016/j.biortech.2019.122101
Liu X, Wu R, Cheng K (2019b) Effects of ammonia nitrogen concentration on partial nitrification and its kinetics. Environ Sci Technol 42(11):47–51,60. https://www.cabdirect.org/cabdirect/abstract/20219906850
Moore ER (2002) Thermomonas haemolytica gen. nov., sp. nov., a gamma-proteobacterium from kaolin slurry. Int J Systematic & Evol Microbiol 52 (Pt 2):473. https://doi.org/10.1099/00207713-52-2-473
Park S, Bae W (2009) Modeling kinetics of ammonium oxidation and nitrite oxidation under simultaneous inhibition by free ammonia and free nitrous acid. Process Biochem 44(6):631–640. https://doi.org/10.1016/j.procbio.2009.02.002
She ZL, Zhao LT, Zhang XL, Jin CJ, Guo L, Yang SY, Zhao YG, Gao MC (2016) Partial nitrification and denitrification in a sequencing batch reactor treating high-salinity wastewater. Chem Eng J 288:207–215. https://doi.org/10.1016/j.cej.2015.11.102
Sheng L, Lei Z, Dzakpasu M, Li YY, Li Q, Chen R (2020) Application of the anammox-based process for nitrogen removal from anaerobic digestion effluent: A review of treatment performance, biochemical reactions, and impact factors. J Water Process Eng , 38. https://doi.org/10.1016/j.jwpe.2020.101595
Shi Z, Qi X, Zeng X, Lu Y, Zhou J, Cui K (2021) Zhang L (2021) A newly isolated bacterium Comamonas sp. XL8 alleviates the toxicity of cadmium exposure in rice seedlings by accumulating cadmium. J Hazard Mater 403:123824. https://doi.org/10.1016/j.jhazmat.2020.123824
Siegmanigra Y, Baryosef S, Gorea A, Avram J (1993) Nosocomial acinetobacter meningitis secondary to invasive procedures: report of 25 cases and review. Clin Infect Dis. https://doi.org/10.1093/clinids/17.5.843
Soliman M, Eldyasti A (2016) Development of partial nitrification as a first step of nitrite shunt process in a sequential batch reactor (SBR) using ammonium oxidizing bacteria (AOB) controlled by mixing regime. Bioresour Technol 221:85–95. https://doi.org/10.1016/j.biortech.2019.122311
Stuti S, Shweena K, Rajni S (2021) Pseudomonas mediated nutritional and growth promotional activities for sustainable food security. Curr Res Microb Sci 2:100084. https://doi.org/10.1016/j.crmicr.2021.100084
Tian WD, An KJ, Ma C, Han XK (2013) Partial nitritation for subsequent anammox to treat high-ammonium leachate. Environ Technol 34(8):1063–1068. https://doi.org/10.1080/09593330.2012.733503
Wang WG, Yan Y, Zhao YH, Shi Q, Wang YY (2020) Characterization of stratified EPS and their role in the initial adhesion of anammox consortia. Water Res 169. https://doi.org/10.1016/j.watres.2019.115223
Watanabe K, Teramoto M, Harayama S (1999) An outbreak of nonflocculating catabolic populations caused the breakdown of a phenol-digesting activated-sludge process. Appl Environ Microbiol 65(7):2813. https://doi.org/10.1128/AEM.65.7.2813-2819.1999
Wei D, Ngo HH, Guo WS, Xu WY, Du B, Wei Q (2018) Partial nitrification granular sludge reactor as a pretreatment for anaerobic ammonium oxidation (Anammox): achievement, performance and microbial community. Bioresour Technol 269:25–31. https://doi.org/10.1016/j.biortech.2018.08.088
Wen RL, Jin Y, Zhang WJ (2020) Application of the Anammox in China—a Review. Int J Environ Res Public Health 17(3):1090. https://doi.org/10.3390/ijerph17031090
Xiao HK, Peng YZ, Zhang Q, Liu Y (2021) Pre-anaerobic treatment enhanced partial nitrification start-up coupled with anammox for advanced nitrogen removal from low C/N domestic wastewater. Bioresour Technol 337:125434. https://doi.org/10.1016/j.biortech.2021.125434
Yamamoto T, Takaki K, Koyama T, Furukawa K (2008) Long-term stability of partial nitritation of swine wastewater digester liquor and its subsequent treatment by Anammox. Bioresour Technol 99(14):6419–6425. https://doi.org/10.1016/j.biortech.2007.11.052
Zhang L, Yang JC, Hira D, Fujii T, Furukawa K (2011) High-rate partial nitrification treatment of reject water as a pretreatment for anaerobic ammonium oxidation (anammox). Bioresour Technol 102(4):3761–3767. https://doi.org/10.1016/j.biortech.2010.12.008
Zhang W, Yuhan D, Dunqiu W, Heng C, Yue J (2016) Treatment of high-strength rare-earth ammonia wastewater with a two-stage anaerobic ammonium oxidation (Anammox) process. Glob Nest J 18(186):867–74. https://www.cabdirect.org/cabdirect/abstract/20173023726
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This research was funded by the Guangxi Natural Science Foundation, grant number 2019GXNSFFA245017.
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Material preparation, data collection, and analysis were performed by Yue Jin. The first draft of the manuscript was written by Yue Jin and Xuli Zhang. Prof. Haixiang Li directed the experiment plan and data analysis; Zhicheng Wu set up the experimental reactor and carried out the data collection; the manuscript revision and submission were completed by Wenjie Zhang. And all authors contributed to the study conception and design; all authors commented on previous versions of the manuscript, read, and approved the final manuscript.
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Jin, Y., Zhang, X., Li, H. et al. High-rate partial nitritation as a pretreatment of anammox process. Environ Sci Pollut Res 30, 104592–104602 (2023). https://doi.org/10.1007/s11356-023-29663-7
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DOI: https://doi.org/10.1007/s11356-023-29663-7