Abstract
Removal of synthetic textile dyes poses a challenge to the textile industry and a threat to the environment’s flora and fauna. These dyes are recalcitrant and not very amenable to physical and chemical techniques of degradation. Hence, several studies on alternative bioremediation methods involving plants, plant roots, single microbes, or a consortium of microbes for the decolorization of dyes have been carried out. In the present study, potent bacteria for dye decolorization were isolated from rhizospheric soil of mangrove plants collected from Kamothe, Navi Mumbai, India. Of the 20 isolates obtained after enrichment, seven isolates were used for further screening of efficient decolorization ability in minimal basal media containing 10% glucose, 2.5% trace metal solution, and 0.1% of Methyl Orange (MO) dye concentration. Physiological parameters to optimize the decolorization of dye at optimum pH, temperature, and incubation time were studied for all the seven isolates. UV–vis and Fourier transform infrared spectroscopy were used to investigate dye decolorization. The seven isolates were characterized morphologically, biochemically, and molecular identification of these bacterial isolates was performed by 16S rRNA sequence analysis. The isolates were identified as Bacillus paramycoides, Pseudomonas taiwanensis, Citrobacter murliniae, Acinetobacter pitti, Exiguobacterium acetylicum, Psychrobacter celer, and Aeromonas taiwanensis. Out of these, Aeromonas taiwanensis has shown exceptional capacity by ~ 100% decolorization of azo dye in minimum time.
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Data availability
All the data generated during the study has been uploaded on the public platform NCBI under the accession numbers MT256266, MT256267, MT256269, MT256270, MT256271, MT256273, and MT256274.
Abbreviations
- MBM:
-
Minimal basal medium
- CFE:
-
Cell-free extract
- MO:
-
Methyl Orange
- MR:
-
Methyl Red
- CR:
-
Congo Red
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Acknowledgements
The author is grateful to the D Y Patil Deemed To Be University’s School of Biotechnology and Bioinformatics in Navi Mumbai, India, for providing research facilities for this study.
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AM: Experiment perform, Compilation of data, Manuscript drafting, Graphical abstract preparation.
SS: Inception of the project, planning and execution of the experiment, Manuscript editing, and reviewing.
NP: Phylogenetic analysis, collection of soil sample.
JP: Inception of the project, planning of the project, collection of soil samples.
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Highlights
• Collection of soil samples from rhizospheric and non-rhizospheric soil region of the mangroves plant.
• Enrichment of soil sample in media containing azo dye for dye decolorization.
• Screening and isolation of potential dye decolourizers.
• Optimize the condition for dye decolorization.
• FTIR analysis and application-based studies.
• Identification of microbes using 16SrRNA.
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Modi, A., Singh, S., Patki, J. et al. Screening and identification of azo dye decolorizers from mangrove rhizospheric soil. Environ Sci Pollut Res 29, 83496–83511 (2022). https://doi.org/10.1007/s11356-022-21610-2
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DOI: https://doi.org/10.1007/s11356-022-21610-2