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Effects of chiral herbicide dichlorprop on Arabidopsis thaliana metabolic profile and its implications for microbial communities in the phyllosphere

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Abstract

Dichlorprop (2-(2,4-dichlorophenoxy) propionic acid, DCPP), a commonly used herbicide for weed control, can be residually detected in soil. It is still unclear whether chiral DCPP exerts an enantioselective adverse effect on plant metabolism and the microbial community of the phyllosphere. In this study, we selected Arabidopsis thaliana as a model plant to explore the effects of R- and S-DCPP enantiomers on plant physiological activities, metabolism, and associated changes in the phyllosphere microbial community. Results indicated that the fresh weight of plants decreased by 37.6% after R-DCPP treatment, whereas it increased by 7.6% after S-DCPP treatment. The R-DCPP enantiomer also caused stronger disturbance to leaf morphology, mesophyll cell structure, and leaf metabolites compared with S-DCPP. GC–MS analysis of DCPP-treated Arabidopsis leaves pointed out a differential profile mostly in carbohydrates, organic acids, and fatty acids, between S-DCPP and R-DCPP treatments. The diversity of phyllospheric microorganisms decreased and the stability of microbial community in the phyllosphere increased after R-DCPP treatment, whereas the opposite result was detected after S-DCPP exposure. The correlation analysis revealed that chiral herbicides may affect microbial communities in the phyllosphere by influencing leaf metabolism, while sugars and terpenoids were considered the main factors in reshaping the microbial community structure in the phyllosphere. Our study provides a new perspective for evaluating the effect of residual DCPP enantiomers on plant physiology and corresponding phyllosphere microorganism changes via the regulation of leaf metabolism, and clarifies the ecological risk of DCPP enantiomer application in agriculture.

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Acknowledgements

The authors thank the editor and anonymous reviewers for their constructive and valuable comments for enhancing the quality of our manuscript

Funding

This work was financially supported by the National Natural Science Foundation of China (21777144, 21976161, and 41907210).

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Authors and Affiliations

  1. College of Environment, Zhejiang University of Technology, Hangzhou, 310032, People’s Republic of China

    Danyan Qiu, Yizhi Ye, Mingjing Ke, Nuohan Xu, Zhenyan Zhang, Fan Zhang, Jian Kang, Yitian Yu, Tao Lu & Haifeng Qian

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  1. Danyan Qiu
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  2. Yizhi Ye
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  4. Nuohan Xu
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  8. Yitian Yu
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  9. Tao Lu
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  10. Haifeng Qian
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Contributions

Danyan Qiu: conceptualization, data curation, formal analysis, writing—original draft. Yizhi Ye: methodology, investigation, validation, formal analysis. Mingjing Ke: investigation, formal analysis. Nuohan Xu: investigation, formal analysis. Zhenyan Zhang: methodology, software. Fan Zhang: methodology, investigation. Jian Kang: investigation, software. Tao Lu: project administration, formal analysis. Haifeng Qian: project administration, visualization, writing—review and editing, funding acquisition.

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Correspondence to Haifeng Qian.

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Qiu, D., Ye, Y., Ke, M. et al. Effects of chiral herbicide dichlorprop on Arabidopsis thaliana metabolic profile and its implications for microbial communities in the phyllosphere. Environ Sci Pollut Res 29, 28256–28266 (2022). https://doi.org/10.1007/s11356-021-17936-y

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