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Novel immunochromatographic strip assay based on up-conversion nanoparticles for sensitive detection of imidacloprid in agricultural and environmental samples

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Abstract

Neonicotinoid insecticides are widely used in agriculture for pest control, but the pesticide residues in environmental and agricultural products were a big threat to the health of non-target organisms. In this study, a new immunochromatographic strip test was established for the rapid detection of imidacloprid residue, a neonicotinoid insecticide, based on up-conversion nanoparticles (UCNPs) coupled with the monoclonal antibody against imidacloprid. Under optimal conditions, the half inhibitory concentration (IC50), detection limit, and the linear range of this strip were 8.37 ng/mL, 0.45 ng/mL, and 0.97–250 ng/mL. The strip test could be completed in 30 min. The average recoveries of imidacloprid spiked in water, Chinese cabbages, cucumber, honey, and tea samples were 70.1~101.8%, with coefficient of variations less than 18.9%. The strip was used to test real samples and verified by UPLC-MS/MS method with the good agreement (R2 was 0.9825), indicating this novel strip immunoassay is accurate and reliable.

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All data generated or analyzed during this study are included in this published article and its supplementary information files.

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Funding

This study was financially supported by the Key Research and Development Program in Zhejiang Province (2020C02024-2) and National Key Research and Development Program of China (2018YFF0215205).

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Authors and Affiliations

  1. Institute of Pesticide and Environmental Toxicology, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, 310058, China

    Xinying Lu, Yang Chen, Rubing Zou, Fangfang Si, Ying Zhao, Guonian Zhu & Yirong Guo

  2. College of Life Sciences, China Jiliang University, Hangzhou, 310018, China

    Mingzhou Zhang

Authors
  1. Xinying Lu
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  2. Yang Chen
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  3. Rubing Zou
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  4. Fangfang Si
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  5. Mingzhou Zhang
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  6. Ying Zhao
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  7. Guonian Zhu
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  8. Yirong Guo
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Contributions

Investigation, methodology: Xinying Lu, Yang Chen; formal analysis and investigation: Rubing Zou, FangFang Si; writing—original draft preparation: Xinying Lu; funding acquisition: Mingzhou Zhang; resources: Ying Zhao, Guonian zhu; supervision, conceptualization, writing—review and editing: Yirong Guo

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Correspondence to Yirong Guo.

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Lu, X., Chen, Y., Zou, R. et al. Novel immunochromatographic strip assay based on up-conversion nanoparticles for sensitive detection of imidacloprid in agricultural and environmental samples. Environ Sci Pollut Res 28, 49268–49277 (2021). https://doi.org/10.1007/s11356-021-14143-7

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  • DOI: https://doi.org/10.1007/s11356-021-14143-7

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