Abstract
The short-term effects of coastal untreated effluents from Ushuaia Bay, Beagle Channel, on the biochemical and physiological biomarkers of Mytilus chilensis were assessed. An integrated biomarker response (IBR) index was calculated as a helpful tool to represent the general stress of the experimental organisms. Cultured mussels were exposed during 96 h to three coastal sites impacted by sewage effluents. At the beginning (T0) and every 24 h, mussels were subsampled from each site and different biochemical and physiological biomarkers were determined. There was no mortality registered in the experiments during the 96 h. However, biochemical and physiological biomarkers presented significant variations. Lipid peroxidation mean levels in mussels decreased in mantle and increased in digestive gland with respect to T0 in almost all cases. Acetylcholinesterase activity was inhibited in all sites, reaching a maximal decrease of 35% with respect to T0. Catalase remained stable and glutathione-S-transferase was activated. Oxygen consumption and ammonia excretion rates increased in organisms from two sites and, consequently, O:N ratio decreased, denoting a symptom of stress. IBR values showed the existence of different stress levels between exposed and unexposed mussels. These results exhibited an alteration of the general metabolism of mussels exposed for a short period of time to untreated coastal wastewater, suggesting the use of these organisms as early indicators of changes in the environmental quality of coastal waters of Ushuaia Bay.
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Data availability
The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
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The authors wish to thank anonymous reviewers for their valuable comments on the manuscript.
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This study was supported by Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT PICT N° 1261/2006) granted to Dr. O. Amin.
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Soledad Diodato: Conceptualization, formal analysis, investigation, visualization, writing original draft and writing review and editing. Laura Comoglio: Conceptualization, investigation, supervision and writing review and editing. Carolina Camilion: investigation and methodology. Oscar Amin: conceptualization, project administration, funding acquisition and writing review and editing. Jorge Marcovecchio: supervision and writing review and editing. All authors read and approved the final manuscript.
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Diodato, S., Comoglio, L., Camilion, C. et al. Integrated biomarker response in Mytilus chilensis exposed to untreated urban discharges along the coast of Ushuaia Bay (Beagle Channel, Argentina). Environ Sci Pollut Res 28, 39892–39906 (2021). https://doi.org/10.1007/s11356-021-13587-1
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DOI: https://doi.org/10.1007/s11356-021-13587-1