Abstract
Microcystins (MCs) are cyclic heptapeptide hepatotoxins that are highly soluble in water and can be transferred to farmland through irrigation with potentially substantial effects on crops, especially rice. In order to investigate the possible negative effects of microcystin-LR (MC-LR) on rice, the oxidative stress induced in rice suspension cells exposed to MC-LR at environmentally relevant concentrations (0.05, 0.5, 5.0, and 50.0 μg·L−1) was investigated. Results showed that the exposure to MC-LR at 0.5–50.0 μg·L−1 resulted in a significant decline in viability of rice suspension cells and an increase in malondialdehyde (MDA) contents. In the 50.0-μg·L−1 MC-LR treatment group, the content of MDA was as much as 5.39 times that of the control group after 6 days of exposure. The excess MDA production indicated that MC-LR exposure has caused lipid peroxidation damage in rice cells, whereas these negative effects could be recovered over time when suspension cells were exposed to low concentration of MC-LR (0.05 μg·L−1). When exposed to MC-LR for 3 days, the O2− content in all treatment groups increased significantly compared with the control group. Additionally, the antioxidant system of rice suspension cells initiated a positive stress response to MC-LR exposure. Indeed, peroxidase (POD) played an active role in the removal of reactive oxygen species (ROS) in rice suspension cells during the early period of exposure, while total superoxide dismutase (T-SOD) was induced after 6 days. Similarly, after 6 days of exposure, the anti-superoxide anion free radical activity (ASAFR), glutathione (GSH), and glutathione-S transferase (GST) in rice suspension cells were higher than that in the control group. These results provided a comprehensive understanding of the exposure time- and dose-dependent oxidative stress induced by the environmentally relevant concentrations of MC-LR in rice suspension cells.
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This work was financially supported by the National Key Research and Development Program of China (2017YFD0801500) and the National Natural Science Foundation of China (21407056).
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Jinlin Jiang contributed to the study conception and design. Material preparation and data collection were performed by Xingcheng Zhu and Renbin Liu. Analysis was performed by Tao Long, Huanchao Zhang, and Shaopo Deng. Shihui Chen and Jinlin Jiang wrote the manuscript. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Chen, S., Jiang, J., Long, T. et al. Oxidative stress induced in rice suspension cells exposed to microcystin-LR at environmentally relevant concentrations. Environ Sci Pollut Res 28, 38393–38405 (2021). https://doi.org/10.1007/s11356-021-13353-3
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DOI: https://doi.org/10.1007/s11356-021-13353-3