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Presence of veterinary antibiotics in livestock manure in two Southeastern Europe countries, Albania and Kosovo

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Abstract

Nowadays, veterinary drug application has become an integral practice in livestock farming. Veterinary antibiotics (VAs) are administered onto animals for therapeutic use; meanwhile, in some countries, they are used for growth promotion. To indicate the level of VAs use in livestock breeding in two countries, Albania and Kosovo, their presence was studied in the animal manure. In total, 38 manure samples, 22 from Kosovo and 16 samples from Albania, belonging to cattle, pig, and poultry, were collected and investigated for the presence of VAs. Seven VAs and 2 metabolites, from the groups of sulfonamides and tetracyclines, were identified by ultra-high pressure liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). The detected antibiotics were sulfadiazine (SDZ), sulfathiazole (STZ), sulfamethazine (SMZ), oxytetracycline (OTC), tetracycline (TC), chlortetracycline (TC), and doxycycline (DOY). VAs were detected in 27% and 31.2% of the manure samples, from Kosovo and Albania, respectively, and the levels ranged from 0.04 to 10.1 mg kg−1. VAs were widely detected (100%) in poultry manure from Kosovo, as well as poultry manure from Albania. The contamination rate ranged from pig manure (25%) to cow manure (66.6%). Sulfonamides were the most commonly detected VAs with maximum concentration of sulfadiazine (10.1 mg kg−1) in poultry manure. Tetracyclines were most widely detected in poultry manure, as well as other animal manures. When it comes to the comparison between the two countries, VAs residues are more frequent per analyzed sample and higher in concentrations in the manure samples from Albania. Therefore, an environmental impact of VAs on both countries may be expected. These results indicate that VAs may enter the local ecosystem through manure application to agriculture and potentially may bring ecological risks.

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Acknowledgments

The authors thank Prof. Dr. Gerd Hamscher and Dr. Astrid Spielmeyer from Justus Liebig University Gießen (Germany) for their support on performing analysis and fruitful discussions.

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Authors and Affiliations

  1. Faculty of Natural Sciences, University of Tirana, Blvd. Zogu 1, No. 25, Tirana, Albania

    Dritan Topi

  2. Food and Veterinary Agency, Prishtina, Kosovo

    Jeton Spahiu

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  1. Dritan Topi
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  2. Jeton Spahiu
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Correspondence to Jeton Spahiu.

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Topi, D., Spahiu, J. Presence of veterinary antibiotics in livestock manure in two Southeastern Europe countries, Albania and Kosovo. Environ Sci Pollut Res 27, 44552–44560 (2020). https://doi.org/10.1007/s11356-020-10341-x

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