Abstract
The present study was led to investigate the defensive role of Terminalia laxiflora extract (TLE) on fipronil (FPN) induced hepatotoxicity and nephrotoxicity in male rats. Rats were administered with TLE (100 mg/kg) against the renal toxicity and hepatotoxicity induced by administration of FPN (10.5 mg/kg) for 30 days. At the end of the experimental period, the serum, liver, and kidneys were harvested and assessed for subsequent analysis. FPN administration to rats resulted in a significant elevation of serum transaminases, urea, and creatinine. Also, FPN-treated groups exhibited a marked reduction in total protein and albumin levels. Compared with the control group, the level of malondialdehyde (MDA) was elevated in groups treated with FPN, whereas superoxide dismutase (SOD), catalase (CAT) activities, and glutathione levels were distinctly reduced in this group. Significant increases in genomic DNA fragmentation and the expression level of the caspase-3 gene were also recorded. The biochemical result was supported by histopathological findings. Co-administration of TLE along with FPN significantly diminished the liver and kidney function tests decreased the level of lipid peroxidation, and enhanced all the antioxidant enzymes, while also diminishing the expression of caspase-3 and DNA laddering, indicating amelioration of DNA damage. These results indicate that TLE plays a vital role in diminishing FPN-induced hepatotoxicity and nephrotoxicity.
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AAK and RM conceived the study and designed the experiment; MI reviewed all the results, carried out data analysis, and drafted the manuscript. MKG performed the molecular assays and wrote the manuscript. AAA collected the plants and prepared the extract. M.A revised the data. All authors read, revised, and approved the final manuscript.
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Khalaf, A.A., Ibrahim, M.A., Galal, M.K. et al. The protective effects of Terminalia laxiflora extract on hepato-nephrotoxicity induced by fipronil in male rats. Environ Sci Pollut Res 27, 39507–39515 (2020). https://doi.org/10.1007/s11356-020-10018-5
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DOI: https://doi.org/10.1007/s11356-020-10018-5