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Ameliorative effect of naringin against thiram-induced tibial dyschondroplasia in broiler chicken

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Abstract

Tetramethyl thiuram disulfide (thiram) is widely used in agricultural production as an insecticide and fungicide, which can also lead to tibial dyschondroplasia (TD) in poultry. TD is characterized by leg disorders and growth performance retardation, and no targeted drugs have been found to treat TD until now. Therefore, the objective of the present study was to explore the ameliorative effect of traditional Chinese medicine naringin on thiram-induced TD chickens. A total of 180 one-day-old Arbor Acres (AA) broiler chickens were randomly divided into three equal groups (n = 60): control group (standard diet), thiram-induced group (thiram 50 mg/kg from day 3 to day 7), and naringin-treated group (naringin 30 mg/kg from day 8 to day 18). During the 18-day experiment, the growth performance, tibial bone parameters, antioxidant property of liver, serum biochemical changes and clinical symptoms were recorded to evaluate the protective effect of naringin in thiram-induced TD broiler chickens. Additionally, mRNA expressions and protein levels of Ihh and PTHrP genes were determined via quantitative real-time polymerase chain reaction and western blot. Administration of naringin showed significant results by alleviating lameness, increased growth performance, recuperated growth plate (GP) width, and improved functions and antioxidant enzyme level of liver in broilers affected by TD. Moreover, naringin treatment restored the development of damaged tibia bone via downregulating Ihh and upregulating PTHrP mRNA and protein expressions. In conclusion, our study determines naringin could be used as an effective medicine to treat TD.

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Funding

The work was supported by the National Natural Science Foundation of China (No. 31873031) and the National Key Research and Development Program of China (Project No. 2017YFD0502200).

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Author notes

  1. Xiong Jiang and Aoyun Li contributed equally to this work.

Authors and Affiliations

  1. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Xiong Jiang, Aoyun Li, Yaping Wang, Mudassar Iqbal, Muhammad Waqas, Hao Yang, Zhixing Li, Khalid Mehmood, Hammad Qamar & Jiakui Li

  2. Hubei Three Gorges Polytechnic, Yichang, 443000, Hubei province, People’s Republic of China

    Xiong Jiang

  3. University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan

    Mudassar Iqbal & Khalid Mehmood

  4. Faculty of Veterinary & Animal Sciences, University of the Poonch, District Poonch, Rawalakot, Azad Jammu & Kashmir, 12350, Pakistan

    Muhammad Waqas

  5. College of Animals Husbandry and Veterinary Medicine, Tibet Agricultural and Animal Husbandry University, Linzhi, 860000, Tibet, People’s Republic of China

    Jiakui Li

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  1. Xiong Jiang
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Correspondence to Jiakui Li.

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Jiang, X., Li, A., Wang, Y. et al. Ameliorative effect of naringin against thiram-induced tibial dyschondroplasia in broiler chicken. Environ Sci Pollut Res 27, 11337–11348 (2020). https://doi.org/10.1007/s11356-020-07732-5

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