Skip to main content
SpringerLink
Log in

Ameliorative effect of Bacillus subtilis, Saccharomyces boulardii, oregano, and calcium montmorillonite on growth, intestinal histology, and blood metabolites on Salmonella-infected broiler chicken

  • Research Article
  • Published:
Environmental Science and Pollution Research Aims and scope Submit manuscript

Abstract

This study evaluated the effects of Bacillus subtilis, Saccharomyces boulardii, oregano, and calcium montmorillonite on the physical growth, intestinal histomorphology, and blood metabolites in Salmonella-challenged birds during the finisher phase. In this study, a total of 600 chicks (Ross 308) were randomly distributed into the following dietary treatments: basal diet with no treatment; infected with Salmonella; T1, infected + avilamycin; T2, infected + Bacillus subtilis; T3, Saccharomyces boulardii; T4, infected + oregano; T5, infected + calcium montmorillonite. Our results indicated that feed consumption, body weight gain, total body weight, and feed conversion ratio increased significantly (P < 0.01) in T1 and T2. Villus width increased significantly (P < 0.01) in T1 while the total area was significantly (P < 0.01) higher in T1 and T2 among the treatment groups. Blood protein was significantly (P < 0.01) high in T3 and T4; however, the glucose concentration was significantly (P < 0.01) high in T2, T3, and T4. The treatments increased significantly (P< 0.01) in the treatment groups compared to the negative control. Aspirate aminotransferase (AST) was significantly (P < 0.05) low in T3 compared to the positive control. In conclusion, the results indicated that supplementation of Bacillus subtilis and calcium montmorillonite improved the production performance compared to other feed additives in broiler chicks infected with Salmonella during the finisher phase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abudabos AM, Alyemni AH, Dafalla YM, Khan RU (2016) The effect of phytogenic feed additives to substitute in-feed antibiotics on growth traits and blood biochemical parameters in broiler chicks challenged with Salmonella typhimurium. Environ Sci Pollut Res 23:24151–24157

    Article  CAS  Google Scholar 

  • Abudabos AM, Alyemni AH, Dafalla YM, Khan RU (2017) Effect of organic acid blend and Bacillus subtilis alone or in combination on growth traits, blood biochemical and antioxidant status in broilers exposed to Salmonella typhimurium challenge during the starter phase. J Appl Anim Res 45:538–542

    Article  CAS  Google Scholar 

  • Abudabos AM, Alyemni AH, Dafalla YM, Khan RU (2018) The effect of phytogenics on growth traits, blood biochemical and intestinal histology in broiler chickens exposed to Clostridium perfringens challenge. J Appl Anim Res 46:691–695

    Article  CAS  Google Scholar 

  • Alhidary IA, Abdelrahman MM, Uallh Khan R, Harron RM (2016) Antioxidant status and immune responses of growing camels supplemented a long acting multi trace minerals rumen bolus. Ital J Anim Sci 15:343–349

    Article  CAS  Google Scholar 

  • Bajpai VK, Baek KH, Kang SC (2012) Control of Salmonella in foods by using essential oils, A review. Food Res Intern 45:722–734

    Article  CAS  Google Scholar 

  • Chand N, Faheem H, Khan RU, Qureshi MS, Alhidary IA, Abudabos AM (2016) Anticoccidial effect of mananoligosacharide against experimentally induced coccidiosis in broiler. Environ Sci Pollut Res 23:14414–14421

    Article  CAS  Google Scholar 

  • Ching C, Cravens RL (2016) Calcium montmorillonite, based dietary supplement attenuates necrotic enteritis induced by Eimeria maxima and Clostridium perfringens in broilers. J Poult Sci 53:329–340

    Article  CAS  Google Scholar 

  • Khan RU, Naz S (2013) Application of probiotics in poultry production. World’s Poultry Sci J 69:621–632

    Article  Google Scholar 

  • Khan RU, Naz S, Nikousefat Z, Tufarelli V, Laudadio V (2012a) Thymus vulgaris; alternative to antibiotics in poultry feed. Worlds Poult Sci 68:401–408

    Article  Google Scholar 

  • Khan RU, Naz S, Nikousefat Z, Tufarelli V, Javdani M, Qureshi MS, Laudadio V (2012b) Potential applications of ginger (Zingiber officinale) in poultry diet. Worlds Poult Sci 68(2):245–252

    Article  Google Scholar 

  • Khan RU, Naz S, Javdani M, Nikousefat Z, Selvaggi M, Tufarelli V, Laudadio V (2012c) The use of turmeric (Curcuma longa) in poultry diets. World’s Poultry Sci J 68(1):97–103

    Article  Google Scholar 

  • Khan RU, Nikousefat Z, Tufarelli V, Naz S, Javdani M, Laudadio V (2012d) Garlic (Allium sativa) supplementation in poultry diet, effect on production and physiology. World’s Poultry Sci J 68:417–424

    Article  Google Scholar 

  • Khan RU, Naz S, Dhama K, Kathrik K, Tiwari R, Abdelrahman MM, Alhidary IA, Zahoor A (2016) Direct-fed microbial, beneficial applications, modes of action and prospects as a safe tool for enhancing ruminant production and safeguarding health. Int J Pharmacol 12:220–231

    Article  CAS  Google Scholar 

  • Lillehoj HS, Lee SH, Park SS, Jeong M, Lim Y, Mathis GF, Lumpkins B, Chi F, Ching C, Cravens RL (2016) Calcium montmorillonite-based dietary supplement attenuates necrotic enteritis induced by Eimeria maxima and Clostridium perfringens in broilers. J Poult Sci 53:329–340

    Article  CAS  Google Scholar 

  • National Research Council (1994) Nutrient requirements of poultry, 9th edn. National Academy of Sciences, Washington, DC

    Google Scholar 

  • Rahman S, Khan S, Chand N, Sadique U, Khan RU (2017) In vivo effects of Allium cepa L. on the selected gut microflora and intestinal histomorphology in broiler. Acta Histochem 119:446–450

    Article  CAS  Google Scholar 

  • SAS (2003) Statistical Analysis System user’s guide; Statistics version 9. SAS Institute, Cary, p 1

    Google Scholar 

  • Shahid SN, Chand RU, Khan SM, Suhail and NA Khan (2015). Alterations in cholesterol and fatty acids composition in egg yolk of Rhode Island Red x Fyoumi Hens Fed with Hemp Seeds (Cannabis sativa L.). Journal of Chemistry, Article ID 362936. https://doi.org/10.1155/2015/362936

  • Tehseen M, Tahir M, Khan RU, Jabbar A, Ahmad B, Ahsan T, Khan MS, Khan S, Abudabos AM (2016) Additive effect of Nigella sativa and Zingiber officinale herbal mixture on performance and cholesterol profile in broiler. Philipp Agric Sci 99:408–413

    Google Scholar 

  • Zia Rahman, S Naz, Khan RU, and Tahir M (2017). An update on the potential application of L-carnitine in poultry. World’s Poultry Science Journal 73: 823–830

Download references

Funding

This study was financially supported by the Deanship of Scientific Research at King Saud University through Research Group Project No. RGP-273.

Author information

Authors and Affiliations

  1. Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, Riyadh, KSA, Saudi Arabia

    Alaeldein M. Abudabos, Hemiar A.A. Alhouri, Ibrahim A. Alhidary & Ayman A. Swelum

  2. Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt

    Mohammed A. Nassan

Authors
  1. Alaeldein M. Abudabos
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hemiar A.A. Alhouri
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ibrahim A. Alhidary
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mohammed A. Nassan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ayman A. Swelum
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Alaeldein M. Abudabos.

Ethics declarations

The study was approved by the departmental committee on ethics and welfare of animals approved by the Department of Animal Production, King Saud University, Saudi Arabia.

Additional information

Responsible editor: Philippe Garrigues

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Abudabos, A.M., Alhouri, H.A., Alhidary, I.A. et al. Ameliorative effect of Bacillus subtilis, Saccharomyces boulardii, oregano, and calcium montmorillonite on growth, intestinal histology, and blood metabolites on Salmonella-infected broiler chicken. Environ Sci Pollut Res 26, 16274–16278 (2019). https://doi.org/10.1007/s11356-019-05105-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11356-019-05105-1

Keywords

Search

Navigation