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γ-H2AX foci as indication for the DNA damage in erythrocytes of medaka (Oryzias latipes) intoxicated with 4-nonylphenol

  • Nanotechnology, Nanopollution, Nanotoxicology and Nanomedicine (NNNN)
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Abstract

The present study aimed to investigate the genotoxicity in erythrocytes induced after exposure of medaka (Oryzias latipes) to 4-nonylphenol (4-NP). Adult female medaka fish were exposed to 4-NP at three sublethal concentrations for 15 days to compare their sensitivity with that of catfish as an aquatic model. Comet assay and γ-H2AX were used as biomarkers to detect DNA damage in erythrocytes. Exposure to 4-NP resulted in an increase in the tail moment in a dose-dependent manner. The highest level of DNA damage was recorded after exposure to 100 μg/l 4-NP. The number of foci was increased after exposure to 4-NP, indicating damage to DNA. The present results confirmed the high level of morphological alterations and apoptosis of erythrocytes detected in the first part of this study. 4-NP induced genotoxic effects in medaka, which were found to be more sensitive than catfish after exposure to 4-nonylphenol.

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Funding

This research was supported in part by the Japan Society for the Promotion of Science (FY2015 JSPS postdoctoral fellowship for overseas researchers) to Alaa El-Din H. Sayed (ID No. P15382).

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Authors and Affiliations

  1. Zoology department, Faculty of Science, Assiut University, Assiut, 71516, Egypt

    Alaa El-Din H. Sayed

  2. Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, 277-8562, Japan

    Alaa El-Din H. Sayed, Tomomi Watanabe-Asaka, Shoji Oda & Hiroshi Mitani

  3. Graduate School of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura, Gunma, 374-0193, Japan

    Shosaku Kashiwada

  4. Research Center of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura, Gunma, 374-0193, Japan

    Shosaku Kashiwada

Authors
  1. Alaa El-Din H. Sayed
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  2. Tomomi Watanabe-Asaka
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  3. Shoji Oda
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  4. Shosaku Kashiwada
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  5. Hiroshi Mitani
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Corresponding author

Correspondence to Alaa El-Din H. Sayed.

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Ethics statement

All experiments and protocols were approved by the Committee on the Ethics of Animal Experiments of The University of Tokyo (Permit Number: C-14-02).

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The authors declare that they have no conflict of interest.

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Responsible editor: Philippe Garrigues

Highlights

• 4-Nonylphenol induced genotoxic effects in erythrocytes of medaka.

• DNA damage was increased as dose-dependent manner.

• γ-H2AX was used for the first time to detect DNA damage after 4-NP exposure.

• Medaka WT (Hd-rR) is a sensitive experimental model to 4-NP.

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Sayed, A.ED.H., Watanabe-Asaka, T., Oda, S. et al. γ-H2AX foci as indication for the DNA damage in erythrocytes of medaka (Oryzias latipes) intoxicated with 4-nonylphenol. Environ Sci Pollut Res 27, 18966–18971 (2020). https://doi.org/10.1007/s11356-018-2985-z

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  • DOI: https://doi.org/10.1007/s11356-018-2985-z

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