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Interaction of TiO2 nanoparticles with proteins from aquatic organisms: the case of gill mucus from blue mussel

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Abstract

To better understand the mechanisms of TiO2 nanoparticle (NP) uptake and toxicity in aquatic organisms, we investigated the interaction of NPs with the proteins found in gill mucus from blue mussels. Mucus is secreted by many aquatic organisms and is often their first line of defense against pathogens, xenobiotics, and other sources of environmental stress. Here, five TiO2 NPs and one SiO2 NP were incubated with gill mucus and run out on a one-dimensional polyacrylamide gel for a comparative qualitative analysis of the free proteins in the mucosal solution and the proteins bound to NPs. We then used nanoscale liquid chromatography coupled with tandem mass spectrometry to identify proteins of interest. Our data demonstrated dissimilar protein profiles between the crude mucosal solution and proteins adsorbed on NPs. In particular, extrapallial protein (EP), one of the most abundant mucus proteins, was absent from the adsorbed proteins. After thermal denaturation experiments, this absence was attributed to the EP content in aromatic amino acids that prevents protein unfolding and thus adsorption on the NP. Moreover, although the majority of the protein corona was qualitatively similar across the NPs tested here (SiO2 and TiO2), a few proteins in the corona showed a specific recruitment pattern according to the NP oxide (TiO2 vs SiO2) or crystal structure (anatase TiO2 vs rutile TiO2). Therefore, protein adsorption may vary with the type of NP.

Proteins with adsorption selectivity as identified from isolated bands

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Acknowledgments

The present study was supported by the Programme Transversal de Toxicologie at the CEA. We would like to thank Drs. Jean Labarre and Géraldine Klein for their assistance with the 1D gels.

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Authors and Affiliations

  1. LIONS, NIMBE, CEA, CNRS, Université Paris-Saclay, CEA Saclay, 91191, Gif-sur-Yvette, France

    Adeline Bourgeault, Aurélie Paquirissamy, Clémence Bénatar, Olivier Spalla, Jean-Philippe Renault & Serge Pin

  2. CNRS, UMR 8587, Laboratoire Analyse et Modélisation pour la Biologie et l’Environnement, 91025, Evry, France

    Véronique Legros, Florence Gonnet & Regis Daniel

  3. Laboratoire Analyse et Modélisation pour la Biologie et l’Environnement, Université Evry-Val-d’Essonne, 91025, Evry, France

    Véronique Legros, Florence Gonnet & Regis Daniel

  4. Collège de France, Laboratoire de Chimie de la Matière Condensée de Paris, Sorbonne Universités, UPMC Univ Paris 06, CNRS, 4 Place Jussieu, 75005, Paris, France

    Corinne Chanéac

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  1. Adeline Bourgeault
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  2. Véronique Legros
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Correspondence to Adeline Bourgeault.

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ESM 1

Details on thermal denaturation experiment. Spectral power of the light used during some exposures. List of identified proteins from selected bands on 1D gels. 1D gels and associated protein names obtained after testing the effect of UV and thermal denaturation. Amino acid composition of proteins of interest. (DOCX 4308 kb)

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Bourgeault, A., Legros, V., Gonnet, F. et al. Interaction of TiO2 nanoparticles with proteins from aquatic organisms: the case of gill mucus from blue mussel. Environ Sci Pollut Res 24, 13474–13483 (2017). https://doi.org/10.1007/s11356-017-8801-3

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