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The study of mechanisms of biological activity of copper oxide nanoparticle CuO in the test for seedling roots of Triticum vulgare

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Abstract

The rapid development of nanotechnology raises questions assessment of their impact on living objects. In the present study, we evaluated the impact of nanoparticle (NP) CuO at concentrations ranging from 0.8 to 63.5 g/l in the test on wheat seedlings Triticum vulgare during 1–72 h. In the viability test (WST-test), cells were isolated from the roots of seedlings T. vulgare, 12 h not observed increase reductase activity after 24 h decreased rate of not more than 19% compared with the control. The number of dead cells in seedlings of T. vulgaris after exposure with CuO nanoparticles to the test with Evans blue increased by 5–15% compared to control. We observed that a significant increase in copper revenues leaves 4.5–8.9 times more in relation to the control and the roots—in 5–9.7 times. During the determined amount of active oxygen species, a significant proportional increase in the total pool of reactive oxygen species (ROS) in roots increased to 27.6% after exposure to NP CuO compared with the control. It is shown that in the introduction in medium, the NP CuO in the doses ranging from 3.2 to 63.5 g/l leads to DNA fragmentation and increases the fragments less than 3000 bp on 51.4–62.8%. The totality of our results influences nanoforms of copper oxide on the amount of ROS, and the viability of the genomic component of the cells shows different mechanisms of damage in the activation of a metabolic reaction, to determine the concentration of nano-CuO.

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Acknowledgements

Research carried was out with the financial support of the Russian Ministry of Education as part of the base part of the state task to carry out research projects in the Orenburg State University (project no. 342).

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Authors and Affiliations

  1. Orenburg State University, 13, Pobedy prospect, Orenburg, Russia, 460018

    Anastasia Mickhailovna Korotkova, Svyatoslav Valeryevich Lebedev & Irina Aleksandrovna Gavrish

  2. Federal State Budget Scientific Institution All-Russian Research Institute of Beef Cattle, 29, 9 Yanvarya street, Orenburg, Russia, 460000

    Anastasia Mickhailovna Korotkova & Svyatoslav Valeryevich Lebedev

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  1. Anastasia Mickhailovna Korotkova
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  2. Svyatoslav Valeryevich Lebedev
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Correspondence to Irina Aleksandrovna Gavrish.

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Korotkova, A.M., Lebedev, S.V. & Gavrish, I.A. The study of mechanisms of biological activity of copper oxide nanoparticle CuO in the test for seedling roots of Triticum vulgare . Environ Sci Pollut Res 24, 10220–10233 (2017). https://doi.org/10.1007/s11356-017-8549-9

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