Abstract
The use of pesticides has been increased along with increasing the farming activities and has caused environmental impacts deleteriously. In particular, non-target organisms including fish can be affected by toxic effects of pesticides. Therefore, the impacts of malathion (MTN) on oxidative stress and sperm quality were investigated in vitro. The MTN concentrations used on this study were 0 (control), 75, 100, and 125 μg/L. Lipid peroxidation (MDA), non-enzymatic (GSH), and enzymatic (SOD, GSH-Px, and CAT) activities in spermatozoa were examined for determination of oxidative stress status. Our findings showed that motility rate and period of sperm cells significantly decreased with exposure to MTN. Biochemical assays revealed that CAT activity and levels of MDA, GSH increased in spermatozoa based on concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to MTN exposure. MTN has disruptive effects on sperm quality and caused to oxidative stress in spermatozoa.
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Acknowledgements
Authors like to express deep thanks to continuous help of Mehmet Kutluyer and Aziz Kutluyer for supply of pesticide standards and giving valuable scientific background and important consultancies. In addition, the authors are grateful to Devrim Altintaş for gently provide the facilities and experimental fish.
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Kocabaş, M., Kutluyer, F., Benzer, F. et al. Malathion-induced spermatozoal oxidative damage and alterations in sperm quality of endangered trout Salmo coruhensis . Environ Sci Pollut Res 25, 2588–2593 (2018). https://doi.org/10.1007/s11356-017-0700-0
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DOI: https://doi.org/10.1007/s11356-017-0700-0