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Ecotoxicological impact assessment of the brine discharges from a desalination plant in the marine waters of the Algerian west coast, using a multibiomarker approach in a limpet, Patella rustica

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Abstract

The aim of our study is to evaluate the impact of Bousfer desalination plant brine discharges on the Algerian west coast, on a natural population of the marine gastropod mollusc Patella rustica. The effects of a chronic exposure to such discharges are complex to understand due to the combined effects of environmental physico-chemical parameters (e.g., high salinity) and different pollutants that can modulate the physiological responses of this species to stress. In this context, we assessed the biological effects in a marine species P. rustica, by a multibiomarker approach that provided information on the health status of organisms in response to such an environmental stress. We measured biomarkers in the whole soft tissues of limpets as indicators of neurotoxicity (AChE activity), oxidative stress (CAT, SOD, GR, and GPx activities), biotransformation (GST), oxidative damage (LPO through TBARS levels), and genotoxicity (CSP 3-like activity). In parallel, hydrological parameters were measured in the Bay of Oran, at four selected sites: site H considered as a “hotspot,” located at Bousfer desalination plant; two other sites E and W, at the east and the west of H respectively; finally, site R “reference” located in Madragh, which is considered as a remote clean site. Our analyses revealed that the activities of antioxidant defense enzymes reached the highest levels in P. rustica collected from site H. The activation of antioxidant defense system in these organisms translated the alteration of their status health, reflecting a level of environmental disruption generated by the desalination plant brine discharges and the high salinity in this area. We also observed that the tissues of limpets collected from site H as well as the two other sites, E and W, had undergone molecular damage, confirmed by the high levels of CSP 3-like activity. This damage resulted from chronic exposure to environmental conditions, potentially genotoxic, due to the desalination plant discharges. The present results indicate the adverse impact of brine effluents from desalination plants on marine fauna and suggest the need for a more consistent approach to environmental management of brine discharges.

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Acknowledgments

This research work is part of a collaborative project, PHC-Maghreb (15MAG23), supported and funded by Campus France. This article is dedicated to Professor Zitouni Boutiba, who left us in November 2016.

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Authors and Affiliations

  1. University of Oran 1 Ahmed Ben Bella, Department of Biology, Laboratoire Réseau de Surveillance Environnementale (LRSE), BP 1524 El M’naouer, 31000, Oran, Algeria

    Meriem Benaissa, Omar Rouane-Hacene & Zitouni Boutiba

  2. University Côte d’Azur, CNRS, ECOMERS, 28 Avenue Valrose BP 71, 06108, Nice Cedex 2, France

    Marielle E. Guibbolini-Sabatier & Christine Risso-De Faverney

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  1. Meriem Benaissa
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  2. Omar Rouane-Hacene
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Correspondence to Omar Rouane-Hacene.

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Benaissa, M., Rouane-Hacene, O., Boutiba, Z. et al. Ecotoxicological impact assessment of the brine discharges from a desalination plant in the marine waters of the Algerian west coast, using a multibiomarker approach in a limpet, Patella rustica . Environ Sci Pollut Res 24, 24521–24532 (2017). https://doi.org/10.1007/s11356-017-0081-4

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