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The relationship between plasma and urinary 8-hydroxy-2-deoxyguanosine biomarkers measured by liquid chromatography tandem mass spectrometry

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Abstract

Although 8-hydroxy-2-deoxyguanosine (8-OHdG) is a widely used promising biomarker of DNA damage, there are concerns about which tissues or body fluids should be sampled. The objective of this study is to evaluate the correlation of DNA oxidative damage biomarkers, 8-OHdG, between blood and urine and risk factors associated with 8OHdG. The study population was recruited from a baseline survey of a worksite lifestyle study including 92 office workers aged 23 to 60 years. A self-administered questionnaire was used to collect information on personal characteristics. The plasma and urinary 8-OHdG was measured by liquid chromatography tandem mass spectrometry (LC-MS/MS). In linear regression, a positive relation was found (p < 0.01) between the log-transformed plasma and urinary 8-OHdG levels adjusted for gender, age, BMI, and smoking status. Our findings showed that age, gender and smoking were significantly associated with plasma 8-OHdG, but not with urinary 8-OHdG. Our results suggest that there is a positive relation between the biomarkers of plasma (steady state DNA damage) and urinary 8-OHdG (total DNA damage). However, the plasma 8-OHdG is more sensitive than urinary 8-OHdG to detect increased oxidative damages induced by risk factors.

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Acknowledgment

This study was partly supported by the research grant from Da Chien General Hospital, Miaoli, Taiwan, and partly supported by National Health Research Institutes, Miaoli, Taiwan, R.O.C.

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Authors and Affiliations

  1. Division of Family Medicine, Department of Family and Community Medicine, Tri-Service General Hospital and School of Medicine, National Defense Medical Center, Taipei, Taiwan, Republic of China

    Chung-Ching Wang & Wei-Liang Chen

  2. Department of Pediatrics, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China

    Chien-Ming Lin

  3. Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan, Republic of China

    Chien-Ming Lin

  4. Department of Public Health, National Defence Medical Center, Taipei, Taiwan, Republic of China

    Ching-Huang Lai & Saou-Hsing Liou

  5. Division of Gastroenterology and Hepatology, Department of Internal Medicine, Taichung Armed Forces General Hospital, Taiping, Taichung, Taiwan, Republic of China

    Ching-Hui Loh

  6. Graduate Institute of Medical Sciences, Chang Jung Christian University, Tainan, Taiwan, Republic of China

    Hong-I Chen

  7. National Institute of Environmental Health Sciences, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County, 35053, Taiwan, Republic of China

    Saou-Hsing Liou

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  1. Chung-Ching Wang
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  2. Wei-Liang Chen
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  3. Chien-Ming Lin
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  4. Ching-Huang Lai
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  5. Ching-Hui Loh
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  7. Saou-Hsing Liou
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Correspondence to Saou-Hsing Liou.

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Responsible editor: Philippe Garrigues

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Wang, CC., Chen, WL., Lin, CM. et al. The relationship between plasma and urinary 8-hydroxy-2-deoxyguanosine biomarkers measured by liquid chromatography tandem mass spectrometry. Environ Sci Pollut Res 23, 17496–17502 (2016). https://doi.org/10.1007/s11356-016-6898-4

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  • DOI: https://doi.org/10.1007/s11356-016-6898-4

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