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Aerobic and anaerobic energy production in juvenile roach (Rutilus rutilus): regulation of glycolytic process by ethofumesate at two temperatures

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Abstract

The aim of this study was to evaluate the coupled impact of an herbicide, ethofumesate, and temperature on the cellular energy metabolism of juvenile roach, especially on the glycolysis pathway. Juvenile roach were exposed to 0, 0.5, 5, and 50 μg/L of ethofumesate for 7 days in laboratory conditions at two temperatures (10 and 17 °C). The energy reserves (carbohydrate, lipid, and protein) were quantified, since the availability of substrates regulates the glycolysis. Then, the glycolysis was studied at the biochemical level by the measurement of the glycolytic flux and at the molecular level with the measurement of the relative expression of four genes encoding for glycolysis enzymes. This study revealed different effect of ethofumesate on the glycolysis pathway according to the temperature of exposure. Indeed, at 10 °C, it appeared that only the molecular regulation level was affected, whereas, at 17 °C, ethofumesate acted on the biochemical level. The differences observed between the two exposures imply the establishment of different strategies in order to maintain to cope with stress according to the temperature of exposure.

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Acknowledgments

This work was funded by the Champagne-Ardenne Region (RISKTOX Program) and by the French Ministry of Ecology and Sustainable Development (Programme 190 post grenelle DEVIL). Authors are grateful to the “Laboratoire Municipal et Régional de Reims” (France) for ethofumesate analyses in water samples. Authors are grateful to Marie Gacoin and Floriane Dantas, students in our laboratory, for the measurement of lipids and carbohydrates. Authors are grateful to laboratory technicians (Laurence Delahaut and Isabelle Bonnard) for their help for fish maintenance and dissections during ethofumesate exposures.

During this project, all experiments were conducted in accordance with the Commission recommendation 2007/526/EC on revised guidelines for the accommodation and care of animals used for experimental and other scientific purposes.

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  1. UMR-I 02 SEBIO, Stress Environnementaux et BIOsurveillance des Milieux Aquatiques, Université de Reims Champagne-Ardenne, BP 1039, 51687, Reims Cedex 2, France

    V. Maes, S. Betoulle, A. Geffard, A. Vettier & E. David

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  1. V. Maes
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Maes, V., Betoulle, S., Geffard, A. et al. Aerobic and anaerobic energy production in juvenile roach (Rutilus rutilus): regulation of glycolytic process by ethofumesate at two temperatures. Environ Sci Pollut Res 24, 6853–6865 (2017). https://doi.org/10.1007/s11356-016-6661-x

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