Abstract
Recent studies suggest that the ecotoxicity of engineered nanoparticles (ENPs) is dependent upon the treatment of ENPs in suspensions (e.g. sonication or use of solvents) and on the mode of exposure to test organisms. We conducted several bioassays with Daphnia magna in order to determine how adverse effects of TiO2 nanoparticles (n-TiO2) are influenced by experimental set-up. Several treatments were applied, including three test media, several treatments of n-TiO2 suspensions (stirring, sonication) and different exposure modes (exposure duration and volume of test suspension). No adverse effects were observed when D. magna were exposed to 50 mL of suspension, regardless of TiO2 concentration (up to 250 mg/L) and exposure duration. Conversely, adverse effects were observed when D. magna were exposed to 2 mL of suspension for 96 h with a 50 % effect concentration EC50 values ranging from 32 mg/L to 82 mg/L. Test media had no significant influence on the outcome of all treatments. For a better mechanistic understanding of the experimental set-up at which adverse effects were observed, the particle size of n-TiO2 in the test media was characterized throughout the test duration. These measurements revealed a fast and strong agglomeration with a secondary particle size in the order of magnitude of micrometers. Our study describes how the effects of n-TiO2 on D .magna are influenced by the duration of exposure and volume of media, highlighting the need for standardization of experimental methods.
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References
Bundschuh M, Zubrod JP, Englert D, Seitz F, Rosenfeldt RR, Schulz R (2011) Effects of nano-TiO2 in combination with ambient UV-irradiation on a leaf shredding amphipod. Chemosphere 85:1563–1567
Clement L, Hurel C, Marmier N (2013) Toxicity of TiO2 nanoparticles to cladocerans, algae, rotifers and plants-effects of size and crystalline structure.Chemosphere 90:1083–1090
Crane M, Handy RD, Garrod J, Owen R (2008) Ecotoxicity test methods and environmental hazard assessment for engineered nanoparticles. Ecotoxicology 17:421–437
Dabrunz A, Duester L, Prasse C, Seitz F, Rosenfeldt R, Schilde C, Schaumann GE, Schulz R (2011) Biological surface coating and molting inhibition as mechanisms of TiO2 nanoparticle toxicity in Daphnia magna. PLoS One 6(5):e20112. doi:10.1371/journal.pone.0020112
Handy RD, Cornelis G, Fernandes T, Tsyusko O, Decho A, Sabo-Attwood T, Metcalfe C, Steevens JA, Klaine SJ, Koelmans AA, Horne N (2012a) Ecotoxicity test methods for engineered nanomaterials: practical experiences and recommendations from the bench. Environ Toxicol Chem 31:15–31
Handy RD, Van den Brink N, Chappel M, Muhling M, Behra R, Dusinska M, Simpson P, Ahtiainen J, Jha AN, Seiter J, Bednar A, Kennedy A, Fernandes T, Riediker M (2012b) Practical considerations for conducting ecotoxicity test methods with manufactured nanomaterials: what have we learnt so far? Ecotoxicology 21:933–972
Heinlaan M, Ivask A, Blinova I, Dubourguier H-C, Kahru A (2008) Toxicity of nanosized and bulk ZnO, CuO and TiO2 to bacteria Vibrio fischeri and crustaceans Daphnia magna and Thamnocephalus platyurus. Chemosphere 71:1308–1316
Hischier R, Walser T (2012) Life cycle assessment of engineered nanomaterials: state of the art and strategies to overcome existing gaps. Sci Total Environ 425:271–282
Hund-Rinke K, Simon M (2006) Ecotoxic effect of photocatalytic active nanoparticles (TiO2) on algae and daphnids. Environ Sci Pollut R13:225–232
ISO 6341:1996 Water quality - determination of the inhibition of the mobility of Daphnia magna straus (cladocera, crustacea)-acute toxicity test
Ji J, Longa Z, Lin D (2010) Toxicity of oxide nanoparticles to green algae Chlorella sp. Chem Eng J 170:525–530
Kahru A, Dubourguier H-C (2010) From ecotoxicology to nanoecotoxicology. Toxicology 269:105–119
Kim KT, Klaine SJ, Cho J, Kim SH, Kim SD (2010) Oxidative stress response of Daphnia magna exposed to TiO2 nanoparticles according to size fraction. Sci Total Environ 408:2268–2272
Kiser MA, Westerhoff P, Benn T, Wang Y, Perez-Rivera J, Hristovski K (2009) Titanium nanomaterial removal and release from wastewater. Environ Sci Technol 43:6757–6763
Klaine SJ, Alvarez PJJ, Batley GE, Fernandes TF, Handy RD, Lyon DY, Mahendra S, McLaughlin MJ, Lead JR (2008) Nanomaterials in the environment: fate, bioavailability and effects. Environ Toxicol Chem 27(9):1825–1851
Lovern SB, Klaper R (2006) Daphnia magna mortality when exposed to titanium dioxide and fullerene (C60) nanoparticles. Environ Toxicol Chem 25:1132–1137
Meißner T, Potthoff A, Richter V (2009) Suspension characterization as important key for toxicological investigations. J Phys Conf Ser 170:012012
Menard A, Drobne D, Jemec A (2011) Ecotoxicity of nanosized TiO2. Review of in vivo data. Environ Pollut 159:677–684
Nel A, Xia T, Mändler L, Li N (2006) Toxic potential of materials at the nanolevel. Science 311:622–627
Organisation for Economic Cooperation and Development (OECD) (2004) Guideline for Testing of Chemicals 202 Daphnia sp., Acute Immobilisation Test. Available: http://www.oecdilibrary.org/environment/
Peralta-Videa JR, Zhao L, Lopez-Moreno ML, De la Rosa G, Hong J, Gardea-Torresdey JL (2010) Nanomaterials and the environment: a review for the biennium 2008-2010. J Hazard Mater 186:1–15
Römer I, White TA, Baalousha M, Chipman K, Viant MR, Lead JR (2011) Aggregation and dispersion of silver nanoparticles in exposure media for aquatic toxicity test. J Chromatogr A1281:4226–4233
Seitz F, Bundschuh M, Rosenfeldt RR, Schulz R (2013) Nanoparticle toxicity in Daphnia magna reproduction studies: the importance of test design. Aquat Toxicol 126:163–168
Seitz F, Rosenfeldt RR, Schneider S, Schulz R, Bundschuh M (2014) Size-, surface- and crystalline structure composition-related effects of titanium dioxide nanoparticles during their aquatic life cycle. Sci Total Environ 493:891–897
Strigul N, Vaccari L, Galdun C, Wazne M, Liu X, Christodoulatos C, Jasinkiewicz K (2009) Acute toxicity of boron, titanium dioxide, and aluminium nanoparticles to Daphnia magna and Vibrio fischeri. Desalination 248:771–782
Suttiponparnit K, Jiang J, Sahu M, Suvachittanont S, Charinpanitkul T, Biswas P (2011) Role of surface area, primary particle size and crystal phase on titanium dioxide nanoparticle dispersion properties. Nanoscale Res Lett 6:27
Wu Q, Nouara A, Li Y, Zhang M, Wang W, Tang M, Ye B, Ding J, Wang D (2013) Comparison of toxicities from three metal oxide nanoparticles at environmental relevant concentrations in nematode Caenorhabditis elegans. Chemosphere 90:1123–1131
Zhu X, Zhu L, Chen Y, Tian S (2009) Acute toxicities of six manufactured nanomaterial suspensions to Daphnia magna. J Nanopart Res 11:67–75
Zhu X, Chang Y, Chen Y (2010) Toxicity and bioaccumulation of TiO2 nanoparticle aggregates in Daphnia magna. Chemosphere 78:209–215
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Salieri, B., Pasteris, A., Baumann, J. et al. Does the exposure mode to ENPs influence their toxicity to aquatic species? A case study with TiO2 nanoparticles and Daphnia magna . Environ Sci Pollut Res 22, 5050–5058 (2015). https://doi.org/10.1007/s11356-014-4005-2
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DOI: https://doi.org/10.1007/s11356-014-4005-2