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Antimutagenic and antigenotoxic potential of grape juice concentrate in blood and liver of rats exposed to cadmium

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Abstract

The aim of this study was to evaluate the antimutagenic and antigenotoxic potential of grape juice concentrate in rodent organs exposed to cadmium chloride intoxication. A total of 15 Wistar rats were distributed into three groups (n = 5), as follows: control group (CTRL; nontreated group), cadmium group (Cd), and cadmium-grape juice group (Cd + GJ). Exposed animals received intraperitoneal injection of cadmium chloride (1.2 mg/kg body weight) diluted in water and, after 15 days, Cd + GJ group received grape juice concentrate for 15 days, by gavage (0.8 mL, 1.18 mg of polyphenols kg−1 day−1). Grape juice concentrate was able to decrease genotoxic effects induced by cadmium in peripheral blood and liver cells as depicted by single cell gel (comet) and micronucleus assays. A decrease for anti-8-hydroxy-20-deoxyguanosine (8OHdG) expression in hepatocytes of animals exposed to cadmium and treated with grape juice concentrate was also detected. Higher CuZn-SOD activity was observed in liver cells of the Cd + GJ group. No remarkable differences were seen regarding Mn-SOD activity among groups. Taken together, our results demonstrate that grape juice concentrate was able to exert antimutagenic and antigenotoxic activities in blood and liver cells of rats exposed to cadmium.

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Acknowledgments

This work was supported by CNPq (National Counsel of Technological and Scientific Development). CFGM and DAR are fellowships from CNPq. GPPJ and VGPS are recipients from Coordination for the Improvement of Higher Level-or Education-Personnel (CAPES).

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None declared.

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Authors and Affiliations

  1. Department of Pathology, Federal University of Sao Paulo, UNIFESP, São Paulo, Brazil

    Carolina Foot Gomes de Moura, Gustavo Protasio Pacheco de Jesus, Celina Tizuko Fujiyama Oshima & Daniel Araki Ribeiro

  2. Department of Biosciences, Federal University of Sao Paulo, UNIFESP, 11060-001, São Paulo, Brazil

    Flávia Andressa Pidone Ribeiro, Victor Hugo Pereira da Silva, Andréa Pittelli Boiago Gollücke, Odair Aguiar Jr. & Daniel Araki Ribeiro

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  1. Carolina Foot Gomes de Moura
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  2. Flávia Andressa Pidone Ribeiro
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Correspondence to Daniel Araki Ribeiro.

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de Moura, C.F.G., Ribeiro, F.A.P., Pacheco de Jesus, G.P. et al. Antimutagenic and antigenotoxic potential of grape juice concentrate in blood and liver of rats exposed to cadmium. Environ Sci Pollut Res 21, 13118–13126 (2014). https://doi.org/10.1007/s11356-014-3257-1

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