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High-intensity interval training with probiotic supplementation decreases gene expression of NF-κβ and CXCL2 in small intestine of rats with steatosis

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Abstract

Purpose

The aim of this study was to investigate the effects of high-intensity interval training (HIIT) with probiotic supplementation (Lactobacillus rhamnosus GG) on gene expression of NF-κβ and CXCL2 in small intestine of rats with hepatic steatosis (induced by tetracycline).

Methods

Forty male Wistar rats (200 ± 20 g) were randomly divided into five groups of (n = 8 in each group): healthy control (HC), hepatic steatosis (HS), HS + HIIT, HS + probiotic (Pro) 5. HS + HIIT + Pro. Tetracycline was gavage at a dose of 140 mg/kg, for 7 days, to induce hepatic steatosis. The HIIT performed 5 sessions per week for 5 weeks on the rodent treadmill. Probiotic groups received 109 CFU/ml of Lactobacillus rhamnosus GG by oral gavage for 5 weeks (daily). ALT and AST were measured to confirm of HS. NF-κβ and CXCL2 genes were measured by Real-Time PCR in small intestine (jejunum).

Results

The results showed that there was a significant difference in gene expression of NF-κβ and CXCL2 (jejunum) between groups (p < 0.001). NF-κβ and CXCL2 mRNA levels in the steatosis group were greater than healthy controls (p < 0.05), while probiotic and HIIT groups had a lower intestinal NF-κβ and CXCL2 mRNA compared to steatosis group (p < 0.05).

Conclusions

Probiotic supplementation with HIIT leads to optimal cellular adaptation and the lower expression of NF-κβ and CXCL2 in the intestinal tissue of rats with fatty liver (steatosis). Due to the role of NF-κβ in regulation of apoptotic genes and proinflammatory cytokines, as well as the effect of CXCL2 on metabolism, insulin function and inflammatory responses, it seems that HIIT and probiotic may be effective in controlling fatty liver and gastrointestinal diseases.

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References

  1. Odiaga JA, Doucette J (2017) Technological media and sedentary behavior in pediatrics. J Nurse Pract 13(1):72–78

    Article  Google Scholar 

  2. Willett WC et al. (2006) Prevention of chronic disease by means of diet and lifestyle changes, in disease control priorities in developing countries, 2nd edition. The International Bank for Reconstruction and Development/The World Bank

  3. Nasr P et al (2020) The amount of liver fat predicts mortality and development of type 2 diabetes in non-alcoholic fatty liver disease. Liver Int 40(5):1069–1078

    Article  CAS  Google Scholar 

  4. Brouwers MC et al (2020) Non-alcoholic fatty liver disease and cardiovascular disease: assessing the evidence for causality. Diabetologia 63:253

    Article  CAS  Google Scholar 

  5. Tarantino G, Balsano C (2020) Gastrointestinal peptides and nonalcoholic fatty liver disease. Curr Opin Endocrinol Diabetes Obes 27(1):11–15

    Article  CAS  Google Scholar 

  6. Gomez-Dominguez E et al (2006) A pilot study of atorvastatin treatment in dyslipemid, non-alcoholic fatty liver patients. Aliment Pharmacol Ther 23(11):1643–1647

    Article  CAS  Google Scholar 

  7. Hallsworth K et al (2011) Resistance exercise reduces liver fat and its mediators in non-alcoholic fatty liver disease independent of weight loss. Gut 60(9):1278–1283

    Article  Google Scholar 

  8. Skrypnik D et al (2016) Effects of endurance and endurance–strength exercise on biochemical parameters of liver function in women with abdominal obesity. Biomed Pharmacother 80:1–7

    Article  CAS  Google Scholar 

  9. Arabzadeh E et al (2020) Alteration of follistatin-like 1, neuron-derived neurotrophic factor, and vascular endothelial growth factor in diabetic cardiac muscle after moderate-intensity aerobic exercise with insulin. Sport Sci Health. https://doi.org/10.1007/s11332-020-00631-9

    Article  Google Scholar 

  10. van der Windt DJ et al (2018) The effects of physical exercise on fatty liver disease. Gene Expr 18(2):89

    Article  Google Scholar 

  11. Hashida R et al (2017) Aerobic vs. resistance exercise in non-alcoholic fatty liver disease: a systematic review. J hepatol 66(1):142–152

    Article  Google Scholar 

  12. Hallsworth K et al (2015) Modified high-intensity interval training reduces liver fat and improves cardiac function in non-alcoholic fatty liver disease: a randomized controlled trial. Clin Sci 129(12):1097–1105

    Article  CAS  Google Scholar 

  13. Pedersen BK, Fischer CP (2007) Beneficial health effects of exercise–the role of IL-6 as a myokine. Trends Pharmacol Sci 28(4):152–156

    Article  CAS  Google Scholar 

  14. Catoire M et al (2014) Identification of human exercise-induced myokines using secretome analysis. Physiol Genom 46(7):256–267

    Article  CAS  Google Scholar 

  15. Sun S-C (2017) The non-canonical NF-κB pathway in immunity and inflammation. Nat Rev Immunol 17(9):545

    Article  CAS  Google Scholar 

  16. Kapil S et al (2016) Small intestinal bacterial overgrowth and toll-like receptor signaling in patients with non-alcoholic fatty liver disease. J Gastroenterol Hepatol 31(1):213–221

    Article  CAS  Google Scholar 

  17. Anuradha S, Rajeshwari K (2005) Probiotics in health and disease. J Indian Acad Clin Med 6(1):67–72

    Google Scholar 

  18. Dilnawaz P, Shakeel M, Tabrez S (2011) A review on probiotics. Int Res J Pharm 2:26–33

    Google Scholar 

  19. Shabana M et al (2012) Influence of rifampicin and tetracycline administration on some biochemical and histological parameters in albino rats. J Basic Appl Zool 65(5):299–308

    Article  CAS  Google Scholar 

  20. Kalaki-Jouybari F et al (2020) High-intensity interval training (HIIT) alleviated NAFLD feature via miR-122 induction in liver of high-fat high-fructose diet induced diabetic rats. Arch Physiol Biochem 126(3):242–249

    Article  CAS  Google Scholar 

  21. Brunt EM et al (1999) Nonalcoholic steatohepatitis: a proposal for grading and staging the histological lesions. Am J Gastroenterol 94(9):2467–2474

    Article  CAS  Google Scholar 

  22. Sakharkar AJ et al (2014) Effects of acute ethanol exposure on anxiety measures and epigenetic modifiers in the extended amygdala of adolescent rats. Int J Neuropsychopharmacol 17(12):2057–2067

    Article  CAS  Google Scholar 

  23. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods 25(4):402–408

    Article  CAS  Google Scholar 

  24. Schnabl B, Brenner DA (2014) Interactions between the intestinal microbiome and liver diseases. Gastroenterology 146(6):1513–1524

    Article  CAS  Google Scholar 

  25. Ley RE et al (2006) Human gut microbes associated with obesity. Nature 444(7122):1022–1023

    Article  CAS  Google Scholar 

  26. Zhang H et al (2009) Human gut microbiota in obesity and after gastric bypass. Proc Natl Acad Sci 106(7):2365–2370

    Article  CAS  Google Scholar 

  27. Luedde T, Schwabe RF (2011) NF-κB in the liver—linking injury, fibrosis and hepatocellular carcinoma. Nat Rev Gastroenterol Hepatol 8(2):108–118

    Article  CAS  Google Scholar 

  28. Sun B, Karin M (2008) NF-κB signaling, liver disease and hepatoprotective agents. Oncogene 27(48):6228–6244

    Article  CAS  Google Scholar 

  29. Taghibeigi Hoseinabadi H et al (2019) Effects of eight weeks of aerobic training on expression levels of the HMGB1-RAGE/TLR4-NF-kB proinflammatory pathway in cardiac tissue of male rats with hyperglycemia. Iran J Endocrinol Metab 20(5):246–252

    Google Scholar 

  30. Chen J, Thomsen M, Vitetta L (2019) Interaction of gut microbiota with dysregulation of bile acids in the pathogenesis of nonalcoholic fatty liver disease and potential therapeutic implications of probiotics. J Cell Biochem 120(3):2713–2720

    Article  CAS  Google Scholar 

  31. Chavez-Tapia NC et al (2015) Current evidence on the use of probiotics in liver diseases. J Funct Foods 17:137–151

    Article  CAS  Google Scholar 

  32. Rodrigues FM et al (2019) Physical exercise alters hepatic morphology of low-density lipoprotein receptor knockout ovariectomized mice. Med Mol Morphol 52(1):15–22

    Article  CAS  Google Scholar 

  33. Ma Y-Y et al (2013) Effects of probiotics on nonalcoholic fatty liver disease: a meta-analysis. World J Gastroenterol 19(40):6911

    Article  Google Scholar 

  34. Paolella G et al (2014) Gut-liver axis and probiotics: their role in non-alcoholic fatty liver disease. World J Gastroenterol 20(42):15518

    Article  CAS  Google Scholar 

  35. Barry JC et al (2017) Short-term exercise training alters leukocyte chemokine receptors in obese adults. Med Sci Sports Exerc 49(8):1631–1640

    Article  CAS  Google Scholar 

  36. Ha J et al (2010) CXC chemokine ligand 2 induced by receptor activator of NF-κB ligand enhances osteoclastogenesis. J Immunol 184(9):4717–4724

    Article  CAS  Google Scholar 

  37. Carlin JL et al (2016) Voluntary exercise blocks Western diet-induced gene expression of the chemokines CXCL10 and CCL2 in the prefrontal cortex. Brain Behav Immun 58:82–90

    Article  CAS  Google Scholar 

  38. Arabzadeh E, Mirdar S, Moradiani H (2016) Nigella sativa supplementation attenuates exercise-induced bronchoconstriction in the maturing rat: a histometric and histologic study. Comp Clin Pathol 25(1):1–5

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Physical Education and Sport Science, Yadegare Imam Khomeini (RAH) Shahre-Rey Branch, Islamic Azad University, Tehran, Iran

    Saba Mohammadi

  2. Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran

    Fatemeh Rostamkhani

  3. Department of Physical Education and Sport Sciences, East Tehran Branch, Islamic Azad University, Tehran, Iran

    Shahin Riyahi Malayeri

  4. Exercise Physiology Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Hossein Shirvani

Authors
  1. Saba Mohammadi
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  2. Fatemeh Rostamkhani
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  3. Shahin Riyahi Malayeri
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  4. Hossein Shirvani
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All authors equally contribute in preparation of this manuscript.

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Correspondence to Hossein Shirvani.

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All experimental protocols were approved by the Ethics Committee of the Baqiyatallah University of Medical Science.

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Mohammadi, S., Rostamkhani, F., Riyahi Malayeri, S. et al. High-intensity interval training with probiotic supplementation decreases gene expression of NF-κβ and CXCL2 in small intestine of rats with steatosis. Sport Sci Health 18, 491–497 (2022). https://doi.org/10.1007/s11332-021-00829-5

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  • DOI: https://doi.org/10.1007/s11332-021-00829-5

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