Abstract
Introduction
Osteonecrosis of the femoral head (ONFH) is a disorder that causes a collapse of the femoral head, requiring subsequent total hip replacement. However, the pathogenesis of ONFH remains largely unclear. Herein, exosome metabolomics analyses were conducted to explore the pathophysiology of ONFH.
Objectives
This study aimed to conduct metabolic profiling of bone-derived exosomes of ONFH.
Methods
30 ONFH patients and 30 femoral neck fracture (FNF) patients were included in this study. Exosomes were harvested from the femoral head by using ultracentrifugation. Ultraperformance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) was performed in combination with multivariate statistical analysis to reveal and provided new insight into identify the global metabolic profile of ONFH.
Results
The results of transmission electron microscope (TEM), nanoparticle tracking analysis (NTA), and Western blots indicated that the microvesicles isolated from the femoral head were exosomes. Several compounds were identified, including lipids and lipid-like molecules, amino acids, peptides, organooxygen compounds. 44 differential metabolites were screened between ONFH and FNF patients. The up-and down-regulation of Riboflavin metabolism, Pantothenate and CoA biosynthesis, Glycerophospholipid metabolism, and Sphingolipid metabolism were associated with ONFH pathophysiology.
Conclusion
Our results suggest that metabolomics has huge prospects for elucidating pathophysiology of ONFH.
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Data Availability
The metabolomics and metadata reported in this paper are available via study identifier MTBLS4920.
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Funding
This work was supported by Chongqing Technology Innovation and Application Development Project (Grant No. cstc2019jscx-msxmX0245).
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GMK and ZJ designed study and performed clinical study and sampling. GMK conducted statistical analysis and wrote the manuscript. ZJ revised the manuscript. All authors have read and agreed to the published version of the manuscript.
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The study was conducted in accordance with the Declaration of Helsinki. All experiments were approved by the Research Ethics Committee of the First Affiliated Hospital of Chongqing Medical University. Written informed consent was obtained from each donor and approved by the Institutional Review of the First Affiliated Hospital of Chongqing Medical University.
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Guo, M., Zhang, J. Metabolomic analysis of bone-derived exosomes in osteonecrosis of the femoral head based on UPLC–MS/MS. Metabolomics 19, 34 (2023). https://doi.org/10.1007/s11306-023-01986-z
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DOI: https://doi.org/10.1007/s11306-023-01986-z