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The effects of NONRATT021972 lncRNA siRNA on PC12 neuronal injury mediated by P2X7 receptor after exposure to oxygen-glucose deprivation

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Abstract

Adenosine triphosphate (ATP) participates in signal transmission by acting on P2X receptors, and the P2X7 receptor is involved in the pathophysiological changes of ischemic injury. The PC12 cell line is a popular model system to study sympathetic neuronal function. Long noncoding RNAs (lncRNAs) are highly expressed in the nervous system and serve as regulatory RNAs. In this study, the effects of NONRATT021972 lncRNA siRNA on P2X7-mediated PC12 neuronal injury after exposure to oxygen-glucose deprivation (OGD) were investigated. Our results showed that the viability of PC12 cells cultured with OGD or the P2X7 agonist BzATP was significantly decreased. Treatment with NONRATT021972 siRNA reversed the decreased viability of PC12 cells under OGD conditions. The upregulated P2X7 mRNA and protein levels in PC12 cells under OGD conditions or BzATP treatment were significantly decreased when pretreated with NONRATT021972 siRNA. Moreover, NONRATT021972 siRNA treatment effectively suppressed the increase in [Ca2+]i induced by OGD or P2X7 agonists (ATP or BzATP) in PC12 cells. Therefore, treatment with NONRATT021972 siRNA may decrease sympathetic neuronal injury induced by ischemia.

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Acknowledgments

These studies were supported by grants (nos. 81570735, 31560276, 81560219, 81560529, 81171184, 31060139, and 81200853) from the National Natural Science Foundation of China, a grant (no. 20151122040105) from the Technology Pedestal and Society Development Project of Jiangxi Province, a grant (no. 20142BAB205028) from the Natural Science Foundation of Jiangxi Province, and grants (nos. GJJ13155 and GJJ14319) from the Educational Department of Jiangxi Province.

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Authors and Affiliations

  1. Department of Physiology, Medical School of Nanchang University, Nanchang, 330006, People’s Republic of China

    Guilin Li, Lifang Zou, Yun Gao, Changshui Xu, Hong Xu, Shuangmei Liu, Shouyu Wang, Yun Xue, Bing Wu, Qiulan Lv, Mofeng Ying, Xi Zhang & Shangdong Liang

  2. Undergraduate student of grade 2012, Medical School of Nanchang University, Nanchang, 330006, People’s Republic of China

    Wei Xie, Shiyao Wen & Qiuyu Xie

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  1. Guilin Li
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  2. Lifang Zou
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  3. Wei Xie
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Correspondence to Shangdong Liang.

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Guilin Li and Lifang Zou are both first authors.

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Li, G., Zou, L., Xie, W. et al. The effects of NONRATT021972 lncRNA siRNA on PC12 neuronal injury mediated by P2X7 receptor after exposure to oxygen-glucose deprivation. Purinergic Signalling 12, 479–487 (2016). https://doi.org/10.1007/s11302-016-9513-8

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