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Effect of electroacupuncture on P2X3 receptor regulation in the peripheral and central nervous systems of rats with visceral pain caused by irritable bowel syndrome

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Abstract

The aim of this study is to investigate the role of the purinergic receptor P2X3 in the peripheral and central nervous systems during acupuncture treatment for the visceral pain of irritable bowel syndrome (IBS). A total of 24 8-day-old Sprague–Dawley (SD) neonatal male rats (SPF grade) were stimulated using colorectal distention (CRD) when the rats were awake. The modeling lasted for 2 weeks with one stimulation per day. After 6 weeks, the rats were randomly divided into three groups of eight each: (1) the normal group (NG, n = 8); (2) the model group (MG, n = 8); and (3) the model + electroacupuncture group (EA, n = 8) that received electroacupuncture at a needling depth of 5 mm at the Shangjuxu (ST37, bilateral) and Tianshu (ST25, bilateral) acupoints. The parameters of the Han’s acupoint nerve stimulator (HANS) were as follows: sparse-dense wave with a frequency of 2/100 Hz, current of 2 mA, 20 min/stimulation, and one stimulation per day; the treatment was provided for seven consecutive days. At the sixth week after the treatment, the abdominal withdrawal reflex (AWR) score was determined; immunofluorescence and immunohistochemistry were used to measure the expression of the P2X3 receptor in myenteric plexus neurons, prefrontal cortex, and anterior cingulate cortex; and, a real-time PCR assay was performed to measure the expression of P2X3 messenger RNA (mRNA) in the dorsal root ganglion (DRG) and spinal cord. After stimulation with CRD, the expression levels of the P2X3 receptor in the inter-colonic myenteric plexus, DRG, spinal cord, prefrontal cortex, and anterior cingulate cortex were upregulated, and the sensitivity of the rats to IBS visceral pain was increased. Electroacupuncture (EA) could downregulate the expression of the P2X3 receptor and ease the sensitivity to visceral pain. The P2X3 receptor plays an important role in IBS visceral pain. The different levels of P2X3 in the peripheral enteric nervous system and central nervous system mediate the effects of the EA treatment of the visceral hyperalgesia of IBS.

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Acknowledgments

This research was funded by the Specialized Research Fund for the Doctoral Program of Higher Education, No. 20123107110008; the National Natural Sciences Foundation of China (No. 81403474); New Century Excellent Talents in University (No. NCET-13-0907); and the National Basic Research Program of China (973 program, No. 2009CB522900).

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The authors declare that there is no conflict of interests regarding the publication of this paper.

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    Authors and Affiliations

    1. Key Laboratory of Acupuncture and Immunological Effects, Shanghai Research Institute of Acupuncture and Meridian, 650 South Wanping Road, Shanghai, 200030, China

      Z. J. Weng, C. L. Zhou, C. Z. Dou, Y. Shi, H. R. Liu & H. G. Wu

    2. Shanghai Qigong Institute, 650 South Wanping Road, Shanghai, 200030, China

      L. Y. Wu

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    1. Z. J. Weng
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    Correspondence to H. R. Liu or H. G. Wu.

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    Z. J. Weng, L. Y. Wu and C. L. Zhou contributed equally to this work.

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    Weng, Z.J., Wu, L.Y., Zhou, C.L. et al. Effect of electroacupuncture on P2X3 receptor regulation in the peripheral and central nervous systems of rats with visceral pain caused by irritable bowel syndrome. Purinergic Signalling 11, 321–329 (2015). https://doi.org/10.1007/s11302-015-9447-6

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    • DOI: https://doi.org/10.1007/s11302-015-9447-6

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