Skip to main content
SpringerLink
Log in

Pigment composition analysis of fruit pulp in the recombinant progenies reveals the polygenic nature of pulp color inheritance in guava (Psidium guajava L.)

  • Original Article
  • Published:
Tree Genetics & Genomes Aims and scope Submit manuscript

Abstract

Guava (Psidium guajava L.) is a nutritionally rich fruit exhibiting white, yellow, pink, and purple variants for pulp color. To understand the genetic and molecular basis of pulp color, 35 F1s obtained from colored/white crosses and their parents were characterized for pulp color. Black guava (BG) had a rare peel and pulp color with the least amount of lycopene, similar to white-pulped parental genotypes. BG/white crosses gave rise to only white progenies while pink/white crosses segregated into pink, yellow, and white F1s. This confirmed that the pigment responsible for color in BG is not lycopene and the pink-pulped parents are heterozygous for the gene(s) governing pulp color. Additional 74 F1s-generated confirmed that the white pulp color inheritance is homozygous recessive in guava. Pigment composition analysis of all the colored F1s (18/ 35) and the three colored parents revealed that lycopene was present in all the 21 samples but at a wider range (0.16–22.56 mg/100 g). Zeaxanthin was also present in all the samples except two F1s. On the contrary, lutein was found only in five F1 progenies but not in any of the pink-pulped parents. Overall, the pigment composition analysis of F1s and their respective parents revealed that the pulp color in guava is a polygenic trait. We identified 12 distinct SNPs between pink and white-pulped guava genotypes in the PSY1 gene. These variations can be used to develop molecular markers for marker assisted selection of pulp color in guava breeding.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Data Availability

All data generated or analysed during this study are included in this published article [and its supplementary information files].

References

  • Ampomah-Dwamena C, McGhie T, Wibisono R, Montefiori M, Hellens RP, Allan AC (2009) The kiwifruit lycopene beta-cyclase plays a significant role in carotenoid accumulation in fruit. J Expt Bot 60:3765–3779

    Article  CAS  Google Scholar 

  • Ampomah-Dwamena C, Driedonks N, Lewis D, Shumskaya M, Chen X, Wurtzel ET, Allan AC (2015) The Phytoene synthase gene family of apple (Malus x domestica) and its role in controlling fruit carotenoid content. BMC Plant Biol 15:185

    Article  PubMed  PubMed Central  Google Scholar 

  • Bhuvaneswari V, Nagini S (2005) Lycopene: a review of its potential as an anticancer agent. Anti Canc Agents Med Chem 27:635

    Google Scholar 

  • Bin-Jumah MN, Nadeem MS, Gilani SJ, Mubeen B, Ullah I, Alzarea SI, Ghoneim MM, Alshehri S, Al-Abbasi FA, Kazmi I (2022) Lycopene: a natural arsenal in the war against oxidative stress and cardiovascular diseases. Antioxidants (basel) 11(2):232

    Article  CAS  PubMed  Google Scholar 

  • Bramley PM (2000) Is lycopene beneficial to human health? Phytochem 54:233–236

    Article  CAS  Google Scholar 

  • Christofer CI, Pogson BJ (2010) Source to sink: regulation of carotenoid biosynthesis in plants. Trends Plant Sci 15:266–274

    Article  Google Scholar 

  • Clotault J, Peltier D, Berruyer R, Thomas M, Briard M, Geoffriau E (2008) Expression of carotenoid biosynthesis genes during carrot root development. J Expt Bot 59:3563–3573

    Article  CAS  Google Scholar 

  • Devitt LC, Fanning K, Dietzgen RG, Holton TA (2010) Isolation and functional characterization of a lycopene β-cyclase gene that controls fruit colour of papaya (Carica papaya L.). J Expt Bot 61:33–39

    Article  CAS  Google Scholar 

  • Dieffenbach CW, Lowe TMJ, Dveksler GS (1995) General concepts for PCR primer design. PCR Methods Appl 3(3):S30-7. https://doi.org/10.1101/gr.3.3.s30

    Article  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12(1):13–15

    Google Scholar 

  • Fantini E, Falcone G, Frusciante S, Giliberto L, Giuliano G (2013) Dissection of tomato lycopene biosynthesis through virus induced gene silencing. Plant Physiol 163:986–998

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Feng C, Feng C, Lin X, Liu S, Li Y, Kang M (2020) A chromosome-level genome assembly provides insights into ascorbic acid accumulation and fruit softening in guava (Psidium guajava). Plant Biotech J. https://doi.org/10.1111/pbi.13498. (Epub ahead of print PMID: 33098334)

    Article  Google Scholar 

  • Flores G, Wu SB, Negrin A, Kennelly EJ (2015) Chemical composition and antioxidant activity of seven cultivars of guava (Psidium guajava) fruits. Food Chem 170:327–335

    Article  CAS  PubMed  Google Scholar 

  • Fraser PD, Truesdale MR, Bird CR, Schuch W, Bramley PM (1994) Carotenoid biosynthesis during tomato fruit development (evidence for tissue-specific gene expression). Plant Physiol 105:405–413

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Fraser LG, Harvey CF, Crowhurst RN, De Silva HN (2002) EST-derived microsatellites from Actinidia species and their potential for mapping. Theor Appl Genet 108:1010–1016

    Article  Google Scholar 

  • González-Aguilar GA, Tiznado-Hernandez ME, Zavaleta-Gatica R, Martınez-Téllez MA (2004) Methyl jasmonate treatments reduce chilling injury and activate the defence response of guava fruits. Biochem Biophys Res Commun 313(3):694–701

    Article  PubMed  Google Scholar 

  • Han Y, Zheng QS, Wei YP, Chen J, Liu R, Wan HJ (2015) In silico identification and analysis of phytoene synthase genes in plants. Genet Mol Res 14:9412–9422

    Article  CAS  PubMed  Google Scholar 

  • Hirschberg J (2001) Carotenoid biosynthesis in flowering plants. Curr Opin Plant Biol 4:210–218

    Article  CAS  PubMed  Google Scholar 

  • Isaacson T, Ronen G, Zamir D, Hirschberg J (2002) Cloning of tangerine from tomato reveals a carotenoid isomerase essential for the production of β-carotene and xanthophylls in plants. Plant Cell 14:333–342

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Just BJ, Santos CAF, Fonseca MEN, Boiteux LS, Oloizia BB, Simon PW (2007) Carotenoid biosynthesis structural genes in carrot (Daucus carota): isolation, sequence-characterization, single nucleotide polymorphism (SNP) markers, and genome mapping. Theor Appl Genet 114:693–704

    Article  CAS  PubMed  Google Scholar 

  • Kuhn DN, Bally ISE, Dillon NL, Innes D, Groh AM, Rahaman J, Ophir R, Cohen Y, Sherman A (2017) Genetic map of mango: a tool for mango breeding. Front Plant Sci 8:577

    Article  PubMed  PubMed Central  Google Scholar 

  • Liu LH, Zabaras D, Bennett LE, Aguas P, Woonton BW (2009) Effects of UV-C, red light and sun light on the carotenoid content and physical qualities of tomatoes during post-harvest storage. Food Chem 115:495–500

    Article  CAS  Google Scholar 

  • Manoharan RK, Jung HJ, Hwang I, Jeong N, Kho KH, Chung MY, Nou IS (2017) Molecular breeding of a novel orange-brown tomato fruit with enhanced beta-carotene and chlorophyll accumulation. Hereditas 154(1):1–8

    Article  PubMed  PubMed Central  Google Scholar 

  • Mittal A, Yadav IS, Arora NK, Boora RS, Mittal M, Kaur P, Erskine W, Chhuneja P, Gill MIS, Singh K (2020) RNA-sequencing based gene expression landscape of guava cv. Allahabad Safeda and comparative analysis to colored cultivars. BMC Genom 21(1):484. https://doi.org/10.1186/s12864-020-06883-6. (PMCID: PMC7364479)

    Article  CAS  Google Scholar 

  • Paine JA, Shipton CA, Chaggar S, Howells RM, Kennedy MJ, Vernon G, Drake R (2005) Improving the nutritional value of Golden Rice through increased pro-vitamin A content. Nat Biotechnol 23:482

    Article  CAS  PubMed  Google Scholar 

  • Phillips MA, Leon P, Boronat A, Rodriguez-Concepción M (2008) The plastidial MEP pathway: unified nomenclature and resources. Trends Plant Sci 13:619–623

    Article  CAS  PubMed  Google Scholar 

  • Polinati RM, Teodoro AJ, Correa MG, Casanova FA, Passos CLA, Silva JL, Fialho E (2022) Effects of lycopene from guava (Psidium guajava L.) derived products on breast cancer cells. Nat Prod Res 36(5):1405–1408

    Article  CAS  PubMed  Google Scholar 

  • Radha T, Mathew L (2007) Fruit crops. New India Publication Agency, New Delhi, India

    Google Scholar 

  • Rodríguez-Villalón A, Gas E, Rodríguez-Concepción M (2009) Colors in the dark: a model for the regulation of carotenoid biosynthesis in etioplasts. Plant Signal Behav 4:965–967

    Article  PubMed  PubMed Central  Google Scholar 

  • Schaub P, Al-Babili S, Drake R, Beyer P (2005) Why is golden rice golden (yellow) instead of red? Plant Physiol 38:441–450

    Article  Google Scholar 

  • Stoll T, Schweiggert U, Schieber A, Carle R (2003) Process for the recovery of a carotene-rich functional food ingredient from carrot pomace by enzymatic liquefaction. Innov Food Sci Emerg Technol 4:415–423

    Article  CAS  Google Scholar 

  • Sun L, Li S, Jiang J, Tang X, Fan X, Zhang Y, Liu C (2020) New quantitative trait locus (QTLs) and candidate genes associated with the grape berry color trait identified based on a high-density genetic map. BMC Plant Biol 20(1):1–13

    Article  CAS  Google Scholar 

  • Tiwari KK, Pattnaik S, Singh A, Sandhu M, Amitha Mithra SV, Abdin MZ, Singh AK, Mohapatra T (2014) Allelic variation in the microsatellite marker locus RM6100 linked to fertility restoration of WA based male sterility in rice. Indian J Genet 74(4):409–413

    Article  CAS  Google Scholar 

  • Tzuri G, Zhou X, Chayut N, Yuan H, Portnoy V, Meir A, Tadmor Y (2015) A “golden” SNP in CmOr governs the fruit flesh color of melon (Cucumis melo). Plant J 82(2):267–279

    Article  CAS  PubMed  Google Scholar 

  • Vasconcelos AG, Amorim ADGN, Dos Santos RC, Souza JMT, de Souza LKM, Araújo TSL, Nicolau LAD, de Lima Carvalho L, de Aquino PEA, da Silva MC, Ropke CD, Soares PMG, Kuckelhaus SAS, Medeiros JR, Leite JRSA (2017) Lycopene rich extract from red guava (Psidium guajava L.) displays anti-inflammatory and antioxidant profile by reducing suggestive hallmarks of acute inflammatory response in mice. Food Res Int 99(2):959–968

    Article  CAS  PubMed  Google Scholar 

  • Wei X, Meng C, Yuan Y, Nath UK, Zhao Y, Wang Z, Yang S, Li L, Niu L, Yao Q, Wei F, Zhang X (2021) CaPSY1 gene plays likely the key role in carotenoid metabolism of pepper (Capsicum annuum) at ripening. Funct Plant Biol 48(2):141–155

    Article  CAS  PubMed  Google Scholar 

  • Welsch R, Arango J, Bär C, Salazar B, Al-Babili S, Beltrán J, Beyer P (2010) Provitamin A accumulation in cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene. Plant Cell 22:3348–3356

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wisutiamonkul A, Ampomah-Dwamena C, Allan AC, Ketsa S (2017) Carotenoid accumulation and gene expression during durian (Durio zibethinus) fruit growth and ripening. Sci Hortic 220:233–242

    Article  CAS  Google Scholar 

  • Yan P, Gao XZ, Shen WT, Zhou P (2011) Cloning and expression analysis of phytoene desaturase and ζ-carotene desaturase genes in Carica papaya. Mol Bio Rep 38:785–791

    Article  CAS  Google Scholar 

  • Zhang JC, Zhou WJ, Qiang XU, Tao NG, Ye JL, Fei GUO, Deng XX (2013) Two lycopene β-cyclases genes from sweet orange (Citrus sinensis L. Osbeck) encode enzymes with different functional efficiency during the conversion of lycopene-to-provitamin A. J Int Agric 12:1731–1747

    Article  Google Scholar 

  • Zhu H, Chen M, Wen Q, Li Y (2015) Isolation and characterization of the carotenoid biosynthetic genes LCYB, LCYE and CHXB from strawberry and their relation to carotenoid accumulation. Sci Hortic 182:134–144

    Article  CAS  Google Scholar 

  • Ding Y, Yang DH, Wang MY, Hao DC, Li WS, Ling P, Xie SQ (2021) Transcriptome analysis of flower color reveals the correlation between SNP and differential expression genes in Phalaenopsis. https://doi:https://doi.org/10.21203/rs.3.rs-209376/v1

  • Singh G (2005) Strategies for improved production in guava. In: Kishun R, Mishra AK, Singh G, Chandra R (eds) Proceeding of 1st International Guava Symposium. CISH, Lucknow, India, pp 26–39

  • Subramanyam MD, Iyer CPA (1982) Varietal improvement and germplasm evaluation in guava. Fruit Research Workshop, Nagpur, India, pp 117–118

  • UPOV (1987) Guidelines for the conduct of tests for distinctness, homogeneity and stability.

Download references

Acknowledgements

This work is supported by ICAR-IARI, New Delhi, 110012, India, and CAAST, NAHEP.

Author information

Author notes

  1. Madhubala Thakre, Hanamant S and Amitha Mithra Sevanthi contributed equally to this manuscript.

Authors and Affiliations

  1. Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Madhubala Thakre, Hanamant S., Rutuparna Senapati, A. Nagaraja & M. K. Verma

  2. ICAR-National Institute for Plant Biotechnology, New Delhi, 110012, India

    Ramkumar M.K. & Amitha Mithra Sevanthi

  3. Division of Food Science and Post-Harvest Technology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Shalini Gaur Rudra

  4. Division of Agricultural Chemicals, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Supradip Saha

  5. Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Gopala Krishnan S.

  6. ICAR-Central Marine Fisheries Research Institute, Kochi, 682 018, India

    Eldho Varghese

Authors
  1. Madhubala Thakre
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hanamant S.
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ramkumar M.K.
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rutuparna Senapati
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shalini Gaur Rudra
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Supradip Saha
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. Nagaraja
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. M. K. Verma
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Gopala Krishnan S.
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Eldho Varghese
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Amitha Mithra Sevanthi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Madhubala Thakre.

Ethics declarations

Conflict of interest

The authors declare no competing interests

Data archiving statement

The data related to this research paper is not submitted to any databases.

Additional information

Communicated by C. Dardick .

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 908 KB)

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Thakre, M., S., H., M.K., R. et al. Pigment composition analysis of fruit pulp in the recombinant progenies reveals the polygenic nature of pulp color inheritance in guava (Psidium guajava L.). Tree Genetics & Genomes 19, 20 (2023). https://doi.org/10.1007/s11295-023-01595-w

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11295-023-01595-w

Keywords

Search

Navigation