Abstract
Coumarin is widely used in personal care products and pharmaceutical industry, which leads to the release of this compound into environment as an emerging contaminant. Here, a promising strain USTB-Z for biodegrading coumarin was successfully isolated from botanical soil and characterized as a potential novel Pseudomonas sp. based on 16S rDNA sequence analysis and orthologous average nucleotide identity tool. Initial coumarin up to 800 mg/L could be completely removed by USTB-Z within 48 h at the optimal culture conditions of pH 7.3 and 30 °C, which indicates that USTB-Z has a strong capacity in coumarin biodegradation. The biodegradation products of coumarin were further investigated using HPLC and Q-TOF LC/MS, and melilotic acid and 2,3-dihydroxyphenylpropionic acid were identified. The draft genome of strain USTB-Z was sequenced by Illumina NovaSeq, and 21 CDSs for NAD (P)-dependent oxidoreductase, 43 CDSs for hydrolase, 1 CDS for FAD-depend monooxygenase, 1 CDS for 3-hydroxycinnamic acid hydroxylase, 21 CDSs for dioxygenase, and 5 CDSs for fumarylacetoacetate (FAA) hydrolase were annotated and correlated to coumarin biodegradation. The present study provides a theoretical basis and microbial resource for further research on the coumarin biodegradation.
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Funding
This work was supported by the National Natural Science Foundation of China (21677011) and the Fundamental Research Funds for the Central Universities (FRF-TP-18-012A1; FRF-TP-17-009A2).
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ZZ and HY conceived and designed the experiments. ZZ performed the experiments. CL was involved in sample preparation. QX, YL, XL, CY and HZ were involved in identification of biodegradation products and genome analysis. Data was statistically analyzed by ZZ. The paper was written by ZZ, reviewed and edited by HY.
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Zhao, Z., Liu, C., Xu, Q. et al. Pathway for biodegrading coumarin by a newly isolated Pseudomonas sp. USTB-Z. World J Microbiol Biotechnol 37, 89 (2021). https://doi.org/10.1007/s11274-021-03055-w
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DOI: https://doi.org/10.1007/s11274-021-03055-w