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Anti-quorum sensing and antibiofilm potential of 1,8-cineole derived from Musa paradisiaca against Pseudomonas aeruginosa strain PAO1

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Abstract

Pseudomonas aeruginosa is one of the vulnerable opportunistic pathogens associated with nosocomial infections, cystic fibrosis, burn wounds and surgical site infections. Several studies have reported that quorum sensing (QS) systems are controlled the P. aeruginosa pathogenicity. Hence, the targeting of QS considered as an alternative approach to control P. aeruginosa infections. This study aimed to evaluate the anti-quorum sensing and antibiofilm inhibitory potential of Musa paradisiaca against Chromobacterium violaceum (ATCC 12472) and Pseudomonas aeruginosa. The methanol extract of M. paradisiacsa exhibits that better antibiofilm potential against P. aeruginosa. Then, the crude methanol extract was subjected to purify by column chromatography and collected the fractions. The mass-spectrometric analysis of a methanol extract of M. paradisiaca revealed that 1,8-cineole is the major compounds. 1, 8-cineole significantly inhibited the QS regulated violacein production in C. violaceum. Moreover, 1,8-cineole significantly inhibited the QS mediated virulence production and biofilm formation of P. aeruginosa without affecting their growth. The real-time PCR analysis showed the downregulation of autoinducer synthase and transcriptional regulator genes upon 1,8-cineole treatment. The findings of the present study strongly suggested that metabolite of M. paradisiaca impedes P. aeruginosa QS system and associated virulence productions.

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Acknowledgements

The authors gratefully thank Dr. G. Ashwinkumar Subramenium for support in discussing and fulfilling the work. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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  1. Center of Advanced Study in Marine Biology, Annamalai University, Tamil Nadu, Parangipettai, 608 502, India

    Vijayakumar Karuppiah, Ramanathan Thirunanasambandham & Gunasekaran Thangaraj

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  1. Vijayakumar Karuppiah
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  2. Ramanathan Thirunanasambandham
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  3. Gunasekaran Thangaraj
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VK Conceptualization of whole work, performed the experiments, data analyzed and co-wrote the manuscript. RT, GT; Performed the experiments, data analyzed and co-wrote the manuscript.

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Correspondence to Vijayakumar Karuppiah.

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Karuppiah, V., Thirunanasambandham, R. & Thangaraj, G. Anti-quorum sensing and antibiofilm potential of 1,8-cineole derived from Musa paradisiaca against Pseudomonas aeruginosa strain PAO1. World J Microbiol Biotechnol 37, 66 (2021). https://doi.org/10.1007/s11274-021-03029-y

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