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Molecular characterization of bmyC gene of the mosquito pupicidal bacteria, Bacillus amyloliquefaciens (VCRC B483) and in silico analysis of bacillomycin D synthetase C protein

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Abstract

A strain of Bacillus amyloliquefaciens (VCRC B483) exhibiting mosquito pupicidal, keratinase and antimicrobial activities was isolated from mangrove forest ecosystem of Andaman and Nicobar Islands. Molecular characterization of the strain showed the presence of lipopeptide encoding bmyC gene. Phylogenetic tree based on protein sequence of this gene exhibited homology with mycosubtilin synthetase of Bacilus atropheus and Iturin synthetase of Bacillus subtilis and B. amyloliquefaciens. This is the first report on the evolutionary conservation of amino acids concerned with the function and structure of bmyC protein of B. amyloliquefaciens. The presence of valine at the 1197th position in our strain was found to be unique and different from the existing strains of B. subtilis and B. amyloliquefaciens. Molecular modelling studies revealed significant changes in the structure of epimerization domain of the bmyC protein with A1197V variation. Crude metabolite of this strain exhibited antifungal activity against Fusarium sp. and Carvularia sp.

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Acknowledgements

The authors are grateful to the Director, Vector Control Research Centre, Puducherry for his encouragement and valuable suggestions. Technical assistance rendered by Mr. S. Bhoopal Chakravarthy, Mrs. K. Vijayalakshmi and contribution of Mr. Y. Srinivas Murty in electronic artwork is gratefully acknowledged.

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Authors and Affiliations

  1. Unit of Microbiology and Molecular Biology, Vector Control Research Centre, Indian Council of Medical Research (ICMR), Puducherry, 605 006, India

    Mathivanan Ashokkumar, Geetha Irudayaraj & Arulsamy Mary Manonmani

  2. Biomedical Informatics Centre, Vector Control Research Centre, Indian Council of Medical Research (ICMR), Puducherry, 605 006, India

    Nandakumar Yellapu

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  1. Mathivanan Ashokkumar
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  2. Geetha Irudayaraj
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Correspondence to Arulsamy Mary Manonmani.

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11274_2018_2498_MOESM1_ESM.pdf

Consurf color coded mutiple sequence alignment of partial bmyC protein (AHW47908) with 50 homologue sequences showing amino acid conservation (PDF 362 KB)

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Ashokkumar, M., Irudayaraj, G., Yellapu, N. et al. Molecular characterization of bmyC gene of the mosquito pupicidal bacteria, Bacillus amyloliquefaciens (VCRC B483) and in silico analysis of bacillomycin D synthetase C protein. World J Microbiol Biotechnol 34, 116 (2018). https://doi.org/10.1007/s11274-018-2498-4

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  • DOI: https://doi.org/10.1007/s11274-018-2498-4

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