Abstract
In bio-based fermentation, the overall bioprocess efficiency is significantly affected by the metabolic burden associated with the expression of complete biosynthetic pathway as well as precursor and cofactor generating enzymes into a single microbial cell. To attenuate such burden by compartmentalizing the enzyme expression, recently synthetic biologists have used coculture or poly-culture techniques for biomolecules synthesis. In this paper, coculture system of two metabolically engineered Escherichia coli populations were employed which comprises upstream module expressing two enzymes converting para-coumaric acid into resveratrol and the downstream module expressing glucosyltransferase to convert the resveratrol into its glucosidated forms; polydatin and resveratroloside. Upon optimization of the initial inoculum ratio of two E. coli populations, 92 mg resveratrol glucosides/L (236 µM) was produced i.e. achieving 84% bioconversion from 280 µM of p-coumaric acid in 60 h by 3 L fed batch fermentor. This is the report of applying coculture system to produce resveratrol glucosides by expressing the aglycone formation pathway and sugar dependent pathway into two different cells.
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Acknowledgements
This work was supported by the Vietnam National Foundation for Science and Technology Development (NAFOSTED) (106-NN.02-2014.25). We are grateful to Dr. Jules Beekwilder (Plant Research International, Wageningen, The Netherlands) for kindly providing pAC-4CL-STS plasmid, Dr. Shin-ichi Ozaki (Department of Biological Sciences, Faculty of Agriculture, Yamaguchi University, Japan) for providing pQE3-PaGT3 plasmid and Dr. Guhan Jayaraman (Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, India) for pSJR3 plasmid, respectively.
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SM and NHT conceived the study, designed experiments, analyzed data, and wrote the manuscript. NTT, DVC and DVQ performed the experiments and NXC performed NMR study and analyzed the NMR data. All authors read and approved the final manuscript.
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Thuan, N.H., Trung, N.T., Cuong, N.X. et al. Escherichia coli modular coculture system for resveratrol glucosides production. World J Microbiol Biotechnol 34, 75 (2018). https://doi.org/10.1007/s11274-018-2458-z
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DOI: https://doi.org/10.1007/s11274-018-2458-z