Abstract
This study investigated the potential anti-fungal mechanisms of sodium dehydroacetate (SD) against Geotrichum citri-aurantii. The results showed that the cell wall integrity of G. citri-aurantii was not affected, whereas the membrane permeability of G. citri-aurantii mycelia was visibly altered by SD. Dramatic morphological changes of the mycelia, such as loss of cytoplasm, plasmolysis, and dissolution of intracellular substances, were observed by scanning electron microscopy and transmission electron microscopy analyses, indicating that the mycelium is severely damaged by the SD treatment. Furthermore, SD apparently induced a decrease in the intracellular ATP content before 30 min of exposure. An increase in the activity of the Na+/K+-ATPase was also observed, indicating that Na+ ions might enter the cell and thus disturb the energy supply. Taken together, this study’s findings suggest that the anti-fungal activity of SD against G. citri-aurantii can be attributed to the disruption of cell membrane permeability and energy metabolism.
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Acknowledgements
This research was supported by the Collaborative Innovation Center of New Chemical Technologies for Environmental Benignity and Efficient Resource Utilization, the Research Foundation of Education Bureau of Hunan Province (No.15A181) and the Natural Science Foundation of Hunan Province (No. 2017JJ2247).
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Tang, X., Ouyang, Q., Jing, G. et al. Antifungal mechanism of sodium dehydroacetate against Geotrichum citri-aurantii. World J Microbiol Biotechnol 34, 29 (2018). https://doi.org/10.1007/s11274-018-2413-z
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DOI: https://doi.org/10.1007/s11274-018-2413-z