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Necrotic and apoptotic cell death induced by Captan on Saccharomyces cerevisiae

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Abstract

Captan is one of the most widely used broad-spectrum fungicide applied to control several early and late diseases of grapes, apples, and other fruits and vegetables, and as other phthalimide fungicides is defined as a multisite compound with thiol-reactivity. Captan can affect non-target organisms as yeasts, modifying microbial populations and fermentation processes. In this study, we asked whether Captan thiol-reactivity and other mechanisms are involved in acute Captan-induced cell death on aerobic growing Saccharomyces cerevisiae. Thus for, we analyze cellular protein and non-protein thiols, cell membrane integrity, reactive oxygen species accumulation, phosphatidylserine externalization, and apoptotic mutants behavior. The results showed that when submitted to acute Captan treatment most cells lost their membrane integrity and died by necrosis due to Captan reaction with thiols. However, part of the cells, even maintaining their membrane integrity, lost their culture ability. These cells showed an apoptotic behavior that may be the result of non-protein thiol depletion and consequent increase of reactive oxygen species (ROS). ROS accumulation triggers a metacaspase-dependent apoptotic cascade, as shown by the higher viability of the yca1-deleted mutant. Together, necrosis and apoptosis are responsible for the high mortality detected after acute Captan treatment of aerobically growing cells of S. cerevisiae.

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Acknowledgements

The authors acknowledge Cytogene Diagnósticos Moleculares Ltda. for access to flow cytometry equipment. F. J. Scariot thanks Coordenação de Aperfeiçoamento de Pessoal de Nível Superior for fellowship support.

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Authors and Affiliations

  1. Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, Rio Grande Do Sul, Brazil

    Fernando J. Scariot, Luciane Jahn, Ana Paula L. Delamare & Sergio Echeverrigaray

  2. Cytogene Diagnósticos Moleculares Ltda., Lajeado, Rio Grande Do Sul, Brazil

    Sergio Echeverrigaray

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  1. Fernando J. Scariot
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  2. Luciane Jahn
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  3. Ana Paula L. Delamare
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  4. Sergio Echeverrigaray
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Correspondence to Sergio Echeverrigaray.

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Scariot, F.J., Jahn, L., Delamare, A.P.L. et al. Necrotic and apoptotic cell death induced by Captan on Saccharomyces cerevisiae . World J Microbiol Biotechnol 33, 159 (2017). https://doi.org/10.1007/s11274-017-2325-3

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  • DOI: https://doi.org/10.1007/s11274-017-2325-3

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