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Characterizing bacterial communities in tilapia pond surface sediment and their responses to pond differences and temporal variations

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Abstract

Bacterial community compositions in the surface sediment of tilapia ponds and their responses to pond characteristics or seasonal variations were investigated. For that, three ponds with different stocking densities were selected to collect the samples. And the method of Illumina high-throughput sequencing was used to amplify the bacterial 16S rRNA genes. A total of 662, 876 valid reads and 5649 operational taxonomic units were obtained. Further analysis showed that the dominant phyla in all three ponds were Proteobacteria, Bacteroidetes, Chloroflexi, and Acidobacteria. The phyla Planctomycetes, Firmicutes, Chlorobi, and Spirochaetae were also relatively abundant. Among the eight phyla, the abundances of only Proteobacteria, Bacteroidetes, Firmicutes, and Spirochaetae were affected by seasonal variations, while seven of these (with the exception of Acidobacteria) were affected by pond differences. A comprehensive analysis of the richness and diversity of the bacterial 16S rRNA gene, and of the similarity in bacterial community composition in sediment also showed that the communities in tilapia pond sediment were shaped more by pond differences than by seasonal variations. Linear discriminant analysis further indicated that the influences of pond characteristics on sediment bacterial communities might be related to feed coefficients and stocking densities of genetically improved farmed tilapia (GIFT).

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Acknowledgments

This research was jointly supported by the Special Fund of Fundamental Scientific Research Business Expense of the Central Public Research Institutes (Grant 2015JBFM12) and the China Agriculture Research System (Grant CARS-49).

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Authors and Affiliations

  1. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Wuxi, Jiangsu Province, 214182, China

    Limin Fan, Gengdong Hu, Shunlong Meng, Chao Song, Liping Qiu, Yao Zheng, Wei Wu, Jianhong Qu, Jiazhang Chen & Pao Xu

  2. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, Jiangsu Province, 214182, China

    Limin Fan, Kamira Barry, Jiazhang Chen & Pao Xu

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  1. Limin Fan
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  2. Kamira Barry
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  3. Gengdong Hu
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  4. Shunlong Meng
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  5. Chao Song
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  6. Liping Qiu
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  7. Yao Zheng
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  8. Wei Wu
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  9. Jianhong Qu
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  10. Jiazhang Chen
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  11. Pao Xu
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Correspondence to Jiazhang Chen or Pao Xu.

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Fan, L., Barry, K., Hu, G. et al. Characterizing bacterial communities in tilapia pond surface sediment and their responses to pond differences and temporal variations. World J Microbiol Biotechnol 33, 1 (2017). https://doi.org/10.1007/s11274-016-2144-y

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