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Biodegradation of Steroid Hormone Estriol by Pseudomonas proteolytica GBPI_Hb61, a Psychrotolerant Himalayan Bacteria

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Abstract

The Pseudomonas proteolytica GBPI_Hb61 had shown estriol degradation potential. The conditions such as pH, temperature, inoculum volume, agitation speed, and compound concentration were optimized for designing the experiments for getting the maximum degradation using the Box-Behnken design. Bacteria were able to degrade 85.23% of 20 mg L−1 estriol when conditions were media pH 7.0, 25 °C temperature, 6% (v/v) inoculum volume, and 130 rpm agitation speed. Degradation intermediates such as 16-hydroxyestrone, 2-hydroxy2-4-dienevaleric acid, and 2-hydroxy-2,4-diene-1,6-dioic acid were identified through metabolite fragment analysis, and enzyme investigation has shown the presence of acetaldehyde dehydrogenase, dioxygenase, and acetyl COA acetyltransferase in GBPI_Hb61 which are reported to be responsible for estrogen degradation in the environment. GBPI_Hb61 was also found capable of removing estriol in soil.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors are grateful to Director GBP-NIHE for extending the facilities and the Department of Science and Technology-Water Technology Initiative (DST-WTI) [DST/TM/WTI/2K15/63] is duly acknowledged for financial support.

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Authors and Affiliations

  1. Center for Land and Water Resource Management, G. B. Pant National Institute of Himalayan Environment, Kosi-Katarmal, Almora, 263643, Uttarakhand, India

    Pooja Thathola & Vasudha Agnihotri

  2. G. B. Pant National Institute of Himalayan Environment-Himachal Regional Centre, Kullu, 175126, Himachal Pradesh, India

    Vasudha Agnihotri

  3. Department of Biotechnology, Graphic Era Deemed to Be University, Bell Road, Clement Town, Dehradun, 248002, Uttarakhand, India

    Anita Pandey

  4. Department of Biotechnology, Kumaun University, Bhimtal Campus, Bhimtal, Nainital, 263136, Uttarakhand, India

    Santosh Kumar Upadhyay

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  1. Pooja Thathola
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Correspondence to Vasudha Agnihotri.

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Thathola, P., Agnihotri, V., Pandey, A. et al. Biodegradation of Steroid Hormone Estriol by Pseudomonas proteolytica GBPI_Hb61, a Psychrotolerant Himalayan Bacteria. Water Air Soil Pollut 235, 289 (2024). https://doi.org/10.1007/s11270-024-07079-4

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