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A Novel Strategy to Reduce the Higher Nitrogen Loading Shock on Anammox Growth Rate and Granule Sludge Stability

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Abstract

Effects of different electric field intensities on the anammox process were investigated. The results showed that the suitable electric field intensity could dramatically enhance the anammox growth rate and granule sludge stability under a higher nitrogen loading shock. The cellular yield and anammox amount of R2 (0.3 V/cm) was significantly enhanced by 186.48%, 183.79%, 146.51%, 131.26%, and 430%, 440.23% than that of R1 (control group), R3 (0.6 V/cm), and R4 (0.9 V/cm), with a nitrogen loading rate of 11.60 kg-N/(m3∙day). Besides, the electric field could stimulate cells to secrete more EPS to resist higher nitrogen loading shock. The protein (PN)/polysaccharide (PS) ratios of R2 were 22.22%, 16.44%, and 36% higher than those of R1, R3, and R4, which was beneficial to granule sludge stability. This study showed that electric field intensity could solve the problem of lower anammox growth rate and granule stability under a higher nitrogen loading shock.

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References

  • Antwi, P., Zhang, D. C., Luo, W. H., Xiao, L. W., Meng, J., Kabutey, F. T., Ayivi, F., & Li, J. Z. (2019). Performance, microbial community evolution and neural network modeling of single-stage nitrogen removal by partial-nitritation/anammox process. Bioresource Technology, 284, 359–372.

    Article  CAS  Google Scholar 

  • Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1), 248–254.

    Article  CAS  Google Scholar 

  • Cao, X., Zhou, X., Xue, M., Chen, J., & Li, S. (2021). Evaluation of nitrogen removal and N2O emission in a novel anammox coupled with sulfite-driven autotrophic denitrification system: Influence of pH. Journal of Cleaner Production, 321, 128984.

    Article  CAS  Google Scholar 

  • Chen, J., Zheng, P., Yu, Y., Tang, C., & Mahmood, Q. (2010). Promoting sludge quantity and activity results in high loading rates in Anammox UBF. Bioresource technology, 101(8), 2700.

    Article  CAS  Google Scholar 

  • Chen, F. M., Li, X., Yuan, Y., & Huang, Y. (2019). An efficient way to enhance the total nitrogen removal efficiency of the Anammox process by S-0-based short-cut autotrophic denitrification. Journal of Environmental Sciences-China, 81, 214–224.

    Article  CAS  Google Scholar 

  • Chen, W., Chen, S., & Wu, J. (2020). Biomass segregation in the granules and flocs affects the role of heterotrophic bacteria in the ANAMMOX process. Chemical Engineering Journal, 392, 123727.

    Article  CAS  Google Scholar 

  • Cheng, B., Bao, J. G., Du, J., Tufail, H., Xu, T., Zhang, Y., & Mao, Q. (2021). Application of electric fields to mitigate inhibition on anammox consortia under long-term tetracycline stress. Bioresource Technology, 341, 125730.

    Article  CAS  Google Scholar 

  • Dapena-Mora, A., Campos, J. L., Mosquera-Corral, A., Jetten, M. S. M., & Méndez, R. (2014). Stability of the ANAMMOX process in a gas-lift reactor and a SBR. Journal of Biotechnology, 110(2), 159.

    Article  Google Scholar 

  • Fan, N.-S., Jin-Jin, F., Huang, D.-Q., Ma, Y.-L., Lu, Z.-Y., Jin, R.-C., & Zheng, P. (2021). Resistance genes and extracellular proteins relieve antibiotic stress on the anammox process. Water Research, 202, 117453.

    Article  CAS  Google Scholar 

  • He, Q. L., Song, Q., Zhang, S. L., Zhang, W., & Wang, H. Y. (2018). Simultaneous nitrification, denitrification and phosphorus removal in an aerobic granular sequencing batch reactor with mixed carbon sources: Reactor performance, extracellular polymeric substances and microbial successions. Chemical Engineering Journal, 331, 841–849.

    Article  CAS  Google Scholar 

  • Huang, X., Wang, Y., Wang, W., Li, B., Zhao, K., Kou, X., Shizhang, W., & Shao, T. (2022). Simultaneous partial nitritation, anammox, and denitrification process for the treatment of simulated municipal sewage in a single-stage biofilter reactor. Chemosphere, 287(P1), 131974.

    Article  CAS  Google Scholar 

  • Jo, Y., Cho, K., Choi, H., & Lee, C. (2020). Treatment of low-strength ammonia wastewater by single-stage partial nitritation and anammox using upflow dual-bed gel-carrier reactor (UDGR). Bioresource Technology, 304, 123023.

    Article  CAS  Google Scholar 

  • Juan-Díaz, X., Pérez, J., & Carrera, J. (2020). Effective dampening of temperature effects in an anammox reactor treating real mainstream wastewater. Journal of Water Process Engineering, 40, 101853.

    Article  Google Scholar 

  • Korzeniewska, E., Sekulska-Nalewajko, J., Gocławski, J., & Kiełbasa, P. (2020). Analysis of changes in fruit tissue after the pulsed electric field treatment using optical coherence tomography. The European Physical Journal Applied Physics, 91(3), 30902.

    Article  Google Scholar 

  • Kosgey, K., Chandran, K., Gokal, J., Kiambi, S. L., Bux, F., & Kumari, S. (2020). Critical analysis of biomass retention strategies in mainstream and sidestream ANAMMOX-Mediated nitrogen removal systems. Environmental Science & Technology, 55, 9.

    Article  Google Scholar 

  • Lee, H., Choi, S., Kim, E., Kim, Y.-N., Lee, J., & Lee, Dong-Un. (2020). Effects of pulsed electric field and thermal treatments on microbial reduction, volatile composition, and sensory properties of orange juice, and their characterization by a principal component analysis. Applied Sciences, 11(1), 186.

    Article  Google Scholar 

  • Liu, S., Lin, C., Diao, X., Meng, L., & Lu, H. (2019). Interactions between tetracycline and extracellular polymeric substances in anammox granular sludge. Bioresource Technology, 293, 122069.

    Article  CAS  Google Scholar 

  • Lv, Y., Pan, J., Huo, T., Li, J., & Liu, S. (2020). Enhance the treatment of low strength wastewater at low temperature with the coexistence system of AnAOB and heterotrophic bacteria: Performance and bacterial community. Science of the Total Environment, 714, 136799.

    Article  CAS  Google Scholar 

  • Niu, J., Feng, Y., Wang, N., Liu, S., Liang, Y., Liu, J., & He, W. (2021). Effects of high ammonia loading and in-situ short-cut nitrification in low carbon-nitrogen ratio wastewater treatment by biocathode microbial electrochemical system. Science of the Total Environment, 755(P2), 142641.

    Article  CAS  Google Scholar 

  • Pan, C., Xu, D., Dong, Z., Li, W., Kang, D., Chen, W., & Zheng, P. (2021). Effect of temperature decrease on anammox granular sludge: Shock and adaptation. Science of The Total Environment, 798, 149242.

    Article  CAS  Google Scholar 

  • Qin, Y., Han, B., Cao, Y., & Wang, T. (2020). Impact of substrate concentration on anammox-UBF reactors start-up. Bioresource Technology, 239, 422–429.

  • Saborimanesh, N., Yerushalmi, L., & Mulligan, C. N. (2020). Fast recovery of a single-stage partial nitrification/anammox-based hybrid bioreactor impacted by a sudden lactose shock load. Bioresource Technology Reports, 12, 100586.

    Article  Google Scholar 

  • Sobotka, D., Tuszynska, A., Kowal, P., Ciesielski, S., Czerwionka, K., & Makinia, J. (2017). Long-term performance and microbial characteristics of the anammox-enriched granular sludge cultivated in a bench-scale sequencing batch reactor. Biochemical Engineering Journal, 120, 125–135.

    Article  CAS  Google Scholar 

  • Sui, Q., Zheng, R., Zhang, J., Di, F., Zuo, F., Zhang, Y., & Wei, Y. (2021). Successful enrichment of anammox consortium in a single-stage reactor at full-scale: The difference in response of functional genes and transcriptional expressions. Chemical Engineering Journal, 426, 131935.

    Article  CAS  Google Scholar 

  • Tiehm, A., Augenstein, T., Ilieva, D., Schell, H., Weidlich, C., & Mangold, K.-M. (2020). Bio-electro-remediation: Electrokinetic transport of nitrate in a flow-through system for enhanced toluene biodegradation. Journal of Applied Electrochemistry, 40(6), 1263.

    Article  Google Scholar 

  • Wang, G., Zhang, D., You, X., Hua, Y., & Dai, X. (2018). Comparing two start up strategies and the effect of temperature fluctuations on the performance of mainstream anammox reactors. Chemosphere, 209, 632.

    Article  CAS  Google Scholar 

  • Wang, X. T., Yang, H., Yang, S., & Liu, X. Y. (2020). Characteristics and mechanism of anammox granular sludge with different granule size in high load and low rising velocity sewage treatment. Bioresource Technology, 312, 123608.

    Article  CAS  Google Scholar 

  • Wang, H., Peng, L., Mao, N., Geng, J., Ren, H., & Ke, X. (2021). Effects of Fe3+ on microbial communities shifts, functional genes expression and nitrogen transformation during the start-up of Anammox process. Bioresource Technology, 320, 124326.

    Article  CAS  Google Scholar 

  • Xue, Y., Ma, H., Kong, Z., Guo, Y., & Li, Y.-Y. (2020). Bulking and floatation of the anammox-HAP granule caused by low phosphate concentration in the anammox reactor of expanded granular sludge bed (EGSB). Bioresource Technology, 310, 123421.

    Article  CAS  Google Scholar 

  • Yang, S., Xu, S., Boiocchi, R., Mohammed, A., Li, X., Ashbolt, N. J., & Liu, Y. (2020). Long-term continuous partial nitritation-anammox reactor aeration optimization at different nitrogen loading rates for the treatment of ammonium rich digestate lagoon supernatant. Process Biochemistry, 99, 139–146.

    Article  CAS  Google Scholar 

  • Yildiz, S., Pokhrel, P. R., Unluturk, S., & Barbosa-Cánovas, G. V. (2019). Identification of equivalent processing conditions for pasteurization of strawberry juice by high pressure, ultrasound, and pulsed electric fields processing. Innovative Food Science and Emerging Technologies, 57, 102195.

    Article  CAS  Google Scholar 

  • Zhang, C., Li, L., Xiaomin, H., Wang, F., Qian, G., Qi, N., & Zhang, C. (2019a). Effects of a pulsed electric field on nitrogen removal through the ANAMMOX process at room temperature. Bioresource Technology, 275, 225.

  • Zhang, C., Li, L., Wang, Y., & Xiaomin, H. (2019b). Enhancement of the ANAMMOX bacteria activity and granule stability through pulsed electric field at a lower temperature (16 ± 1 °C). Bioresource technology, 292, 121960.

  • Zhang, Q., Zhang, X., Bai, Y.-H., Xia, W.-J., Ni, S.-K., Qing-Yuan, W., Fan, N.-S., Huang, B.-C., & Jin, R.-C. (2020a). Exogenous extracellular polymeric substances as protective agents for the preservation of anammox granules. Science of the Total Environment, 747, 141464. (prepublish).

  • Zhang, L., Wang, Y., Soda, S., He, X., Hao, S., You, Y., & Peng, Y. (2020b). Effect of fulvic acid on bioreactor performance and on microbial populations within the anammox process. Bioresource Technology, 318, 124094.

  • Zhang, J., Peng, Y. Z., Zhang, L. H., Li, J., Wei, J., Zheng, Z. M., & Zhang, K. (2021). Improving the resistance of Anammox granules to extreme pH shock: The effects of denitrification sludge EPS enhanced by a fluctuating C/N ratio cultivation on granules. Science of the Total Environment, 763, 144610.

    Article  CAS  Google Scholar 

  • Zhao, W., Vermace, R. R., Mattes, T. E., & Just, C. (2020). Impacts of ammonia loading and biofilm age on the prevalence of nitrogen-cycling microorganisms in a full-scale submerged attached-growth reactor. Water environment research: A research publication of the Water Environment Federation, 93(5), 787.

    Article  Google Scholar 

Download references

Acknowledgements

This research was financially supported by the Major Science and Technology Program for Water Pollution Control and Treatment (2013ZX07202-010) and the key research and development plan guidance plan project of Liaoning province(2019JH8/10300103).

Funding

This research was financially supported by the Major Science and Technology Program for Water Pollution Control and Treatment (2013ZX07202-010) and the Key Projects of Liaoning Provincial Department of Education (LJKZZ20220081).

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Authors and Affiliations

  1. Sch Municipal & Environm Engn, Shenyang Jianzhu University, Shenyang, 110168, People’s Republic of China

    Chi Zhang, Hongjv Sha & Ze Lv

  2. School of Resources & Civil Engineering, Northeastern University, Shenyang, 110819, People’s Republic of China

    Chi Zhang & Xiaomin Hu

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  1. Chi Zhang
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  2. Hongjv Sha
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Contributions

Chi Zhang: conceptualization, data curation, formal analysis, funding acquisition, project administration, and writing—original draft. Hongjv Sha: data curation, formal analysis, and methodology. Ze Lv: data curation and formal analysis. Xiaomin Hu: funding acquisition and resources, investigation, methodology, supervision, and writing—review and editing.

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Correspondence to Xiaomin Hu.

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Appendix

Appendix

Figures 6, 7 and 8

Fig. 6
figure 6

The schematic diagram of the three anammox reactors

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Fig. 7
figure 7

The effect of different pulsed electric field intensity on the cell structure

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Fig. 8
figure 8

The effect of different electric field intensity on the anammox granule sludge

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Zhang, C., Sha, H., Lv, Z. et al. A Novel Strategy to Reduce the Higher Nitrogen Loading Shock on Anammox Growth Rate and Granule Sludge Stability. Water Air Soil Pollut 235, 182 (2024). https://doi.org/10.1007/s11270-024-06985-x

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