Abstract
In this study, the biosorption performance of Thamnidium elegans (T. elegans) immobilized on Phragmites australis (P. australis), a new biocomposite (TEPA), was examined for decolorization of water using batch and column mode tests. Various affecting experimental parameters such as pH, biocomposite amount, and stirring speed were examined and optimized by an experimental design. Regression analysis indicated that the findings of the Box-Behnken experimental design (BBD) optimization experiment closely match a quadratic model. ANOVA findings revealed that pH and TEPA amount affected Reactive Blue 49 (RB49) biosorption yield. Optimum experimental RB49 decolorization was achieved with the biosorption yield of 96.51% at the conditions of pH: 1.68, TEPA mass: 53.4 mg, stirring speed: 204 rpm, and contact time: 45 min. RB49 sorption onto TEPA was explained using the Elovich and the pseudo-second-order kinetic and the Freundlich isotherm models. The maximum RB49 sorption capacity was 140.36 mg g−1 at defined optimum conditions. Unloaded and dye-loaded biocomposite sorbents were characterized by SEM and FTIR analysis. The isoelectric point of TEPA was found as pH 1.7 by the zeta potential measurements. Furthermore, the newly developed biocomposite sorbent indicated the promise in terms of decolorizing real wastewater without losing dye sorption ability. Saturation biosorption capacities in column mode were 104.58 and 70.98 mg g−1 in dye solution and real wastewater samples, respectively. TEPA can be considered cost-effective, ecofriendly, and promising alternative adsorbent for decolorization of reactive dye contaminated wastewater, as shown by all the findings.
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References
Adane, T., Adugna, A. T., & Alemayehu, E. (2022). Bentonite blended with bagasse ash as an adsorbent for reactive red 198 dyes. Water Practice and Technology, 17(1), 502–516. https://doi.org/10.2166/wpt.2021.129
Akar, T., Arslan, S., & Akar, S. T. (2013). Utilization of Thamnidium elegans fungal culture in environmental cleanup: A reactive dye biosorption study. Ecological Engineering, 58, 363–370. https://doi.org/10.1016/j.ecoleng.2013.06.026
Akar, T., Aydın, P., Celik, S., & Tunali Akar, S. (2020). Phlebia gigantea cells immobilized on renewable biomass matrix as potential ecofriendly scavenger for lead contamination. Environmental Science and Pollution Research, 27(14), 16177–16188. https://doi.org/10.1007/s11356-020-07889-z
Akar, T., & Celik, S. (2011). Efficient biosorption of a reactive dye from contaminated media by Neurospora sitophila cells—Zea mays silk tissue biomass system. Journal of Chemical Technology & Biotechnology, 86(10), 1332–1341. https://doi.org/10.1002/jctb.2639
Akar, T., Kulcu, A., & Tunali Akar, S. (2013). Effective decolorization potential of Thamnidium elegans: Biosorption optimization, modelling, characterization and application studies. Chemical Engineering Journal, 221, 461–468. https://doi.org/10.1016/j.cej.2013.01.082
Akar, T., Sayin, F., Celik, S., & Tunali Akar, S. (2021). Attached culture of Gibberella fujikuroi for biocomposite sorbent production and ciprofloxacin sequestration applications. Journal of Chemical Technology & Biotechnology, 96(9), 2610–2619. https://doi.org/10.1002/jctb.6804
Akar, T., Sayin, F., Turkyilmaz, S., & Tunali Akar, S. (2017). The feasibility of Thamnidium elegans cells for color removal from real wastewater. Process Safety and Environmental Protection, 105, 316–325. https://doi.org/10.1016/j.psep.2016.11.017
Akar, T., Turkyilmaz, S., Celik, S., & Akar, S. T. (2016). Treatment design and characteristics of a biosorptive decolourization process by a green type sorbent. Journal of Cleaner Production, 112, 4844–4853. https://doi.org/10.1016/j.jclepro.2015.06.049
Akar, T., Uzun, C., Çelik, S., & Akar, S. T. (2018). Biosorption of Basic Blue 7 by fungal cells immobilized on the green-type biomatrix of Phragmites australis spongy tissue. International Journal of Phytoremediation, 20(2), 145–152. https://doi.org/10.1080/15226514.2017.1337075
Aksu, Z., & Gönen, F. (2004). Biosorption of phenol by immobilized activated sludge in a continuous packed bed: Prediction of breakthrough curves. Process Biochemistry, 39(5), 599–613. https://doi.org/10.1016/S0032-9592(03)00132-8
Al-Rub, F. A. A., El-Naas, M. H., Benyahia, F., & Ashour, I. (2004). Biosorption of nickel on blank alginate beads, free and immobilized algal cells. Process Biochemistry, 39(11), 1767–1773. https://doi.org/10.1016/j.procbio.2003.08.002
Ali, I., Kon’kova, T., Liberman, E., Simakina, E., Alothman, Z. A., Alomar, T. S., & Ataul Islam, M. (2022). Preparation and characterization of SnO2-CeO2 nanocomposites: Sorption, modeling and kinetics for azorubine dye removal in water. Journal of Molecular Liquids, 346, 117119. https://doi.org/10.1016/j.molliq.2021.117119
Alibabaei, F., Saebnoori, E., Fulazzaky, M. A., Talaeikhozani, A., Roohi, P., Moghadas, F., Abdullah, N. H., & Alian, T. (2021). An evaluation of the efficiency of odorant removal by sodium ferrate(VI) oxidation. Measurement, 179, 109488. https://doi.org/10.1016/j.measurement.2021.109488
Anfar, Z., Ait Ahsaine, H., Zbair, M., Amedlous, A., Ait El Fakir, A., Jada, A., & El Alem, N. (2020). Recent trends on numerical investigations of response surface methodology for pollutants adsorption onto activated carbon materials: A review. Critical Reviews in Environmental Science and Technology, 50(10), 1043–1084. https://doi.org/10.1080/10643389.2019.1642835
Aniagor, C. O., Afifi, M. A., & Hashem, A. (2021). Modelling of basic blue-9 dye sorption onto hydrolyzed polyacrylonitrile grafted starch composite. Carbohydrate Polymer Technologies and Applications, 2, 100141. https://doi.org/10.1016/j.carpta.2021.100141
Bilal, M., Ihsanullah, I., Shah, U. H., & M. & Younas, M. (2021). Enhanced removal of cadmium from water using bio-sorbents synthesized from branches and leaves of Capparis decidua and Ziziphus mauritiana. Environmental Technology & Innovation, 24, 101922. https://doi.org/10.1016/j.eti.2021.101922
Box, G. E. P., & Behnken, D. W. (1960). Some new three level designs for the study of quantitative variables. Technometrics, 2(4), 455–475. https://doi.org/10.1080/00401706.1960.10489912
Box, G. E. P., & Wilson, K. B. (1992). On the experimental attainment of optimum conditions. In S. Kotz & N. L. Johnson (Eds.), Breakthroughs in statistics: Methodology and distribution (pp. 270–310). New York, NY, Springer.
Celik, S., Duman, N., Sayin, F., Tunali Akar, S., & Akar, T. (2021). Microbial cells immobilized on natural biomatrix as a new potential ecofriendly biosorbent for the biotreatment of reactive dye contamination. Journal of Water Process Engineering, 39, 101731. https://doi.org/10.1016/j.jwpe.2020.101731
Chukki, J., Abinandan, S., & Shanthakumar, S. (2018). Chrysanthemum indicum microparticles on removal of hazardous Congo red dye using response surface methodology. International Journal of Industrial Chemistry, 9(4), 305–316. https://doi.org/10.1007/s40090-018-0160-5
Çelik, S., Tunali Akar, S., Şölener, M., & Akar, T. (2017). Anionically reinforced hydrogel network entrapped fungal cells for retention of cadmium in the contaminated aquatic media. Journal of Environmental Management, 204, 583–593. https://doi.org/10.1016/j.jenvman.2017.08.049
D.V, S., Kumar R, L. & J, S. (2017). Immobilized fungi on Luffa cylindrica: An effective biosorbent for the removal of lead. Journal of the Taiwan Institute of Chemical Engineers, 80, 589–595. https://doi.org/10.1016/j.jtice.2017.08.032
Dubinin, M. M., & Radushkevich, L. V. (1947). The equation of the characteristic curve of activated charcoal. Proceedings of the Academy of Sciences, Physical Chemistry Section, 55, 331–333.
Freundlich, H. M. F. (1906). Über die adsorption in lösungen. Zeitschrift Fur Physikalische Chemie, 57, 385–470.
Fulazzaky, M. A., Nuid, M., Aris, A., Fulazzaky, M., Sumeru, K., & Muda, K. (2019). Mass transfer kinetics of phosphorus biosorption by aerobic granules. Journal of Water Process Engineering, 31, 100889. https://doi.org/10.1016/j.jwpe.2019.100889
Fulazzaky, M. A., Nuid, M., Aris, A., & Muda, K. (2017). Kinetics and mass transfer studies on the biosorption of organic matter from palm oil mill effluent by aerobic granules before and after the addition of Serratia marcescens SA30 in a sequencing batch reactor. Process Safety and Environmental Protection, 107, 259–268. https://doi.org/10.1016/j.psep.2017.02.016
Fulazzaky, M. A., Salim, N. A. A., Khamidun, M. H., Puteh, M. H., Yusoff, A. R. M., Abdullah, N. H., Syafiuddin, A. & Zaini, M. A. A.: (2021). The mechanisms and kinetics of phosphate adsorption onto iron-coated waste mussel shell observed from hydrodynamic column, International Journal of Environmental Science and Technology. https://doi.org/10.1007/s13762-021-03563-0
Ghaedi, M., Hajjati, S., Mahmudi, Z., Tyagi, I., Agarwal, S., Maity, A., & Gupta, V. K. (2015). Modeling of competitive ultrasonic assisted removal of the dyes – methylene blue and Safranin-O using Fe3O4 nanoparticles. Chemical Engineering Journal, 268, 28–37. https://doi.org/10.1016/j.cej.2014.12.090
Gharbani, P. (2018). Modeling and optimization of reactive yellow 145 dye removal process onto synthesized MnOX-CeO2 using response surface methodology. Colloids and Surfaces a: Physicochemical and Engineering Aspects, 548, 191–197. https://doi.org/10.1016/j.colsurfa.2018.03.046
Giordano, E. D. V., Brassesco, M. E., Camiscia, P., Picó, G. A., & Valetti, N. W. (2021). A new alternative and efficient low-cost process for the removal of reactive dyes in textile wastewater by using soybean hull as adsorbent. Water, Air, & Soil Pollution, 232(5), 165. https://doi.org/10.1007/s11270-021-05085-4
Gupta, A., & Balomajumder, C. (2015). Simultaneous removal of Cr(VI) and phenol from binary solution using Bacillus sp. immobilized onto tea waste biomass. Journal of Water Process Engineering, 6, 1–10. https://doi.org/10.1016/j.jwpe.2015.02.004
Ho, Y. S., & McKay, G. (1999). Pseudo-second order model for sorption processes. Process Biochemistry, 34(5), 451–465. https://doi.org/10.1016/S0032-9592(98)00112-5
Hu, C., Yu, J. C., Hao, Z., & Wong, P. K. (2003). Photocatalytic degradation of triazine-containing azo dyes in aqueous TiO2 suspensions. Applied Catalysis b: Environmental, 42(1), 47–55. https://doi.org/10.1016/S0926-3373(02)00214-X
Ihsanullah, I., Bilal, M. & Jamal, A.: (2022). Recent developments in the removal of dyes from water by starch-based adsorbents, The Chemical Record, e202100312. https://doi.org/10.1002/tcr.202100312
Ihsanullah, I., Jamal, A., Ilyas, M., Zubair, M., Khan, G., & Atieh, M. A. (2020). Bioremediation of dyes: Current status and prospects. Journal of Water Process Engineering, 38, 101680. https://doi.org/10.1016/j.jwpe.2020.101680
Iqbal, M., & Saeed, A. (2007). Biosorption of reactive dye by loofa sponge-immobilized fungal biomass of Phanerochaete chrysosporium. Process Biochemistry, 42(7), 1160–1164. https://doi.org/10.1016/j.procbio.2007.05.014
Islam, M. A., Ali, I., Karim, S. M. A., Hossain Firoz, M. S., Chowdhury, A.-N., Morton, D. W., & Angove, M. J. (2019). Removal of dye from polluted water using novel nano manganese oxide-based materials. Journal of Water Process Engineering, 32, 100911. https://doi.org/10.1016/j.jwpe.2019.100911
Jabeen, A., & Bhatti, H. N. (2021). Adsorptive removal of reactive green 5 (RG-5) and direct yellow 50 (DY-50) from simulated wastewater by Mangifera indica seed shell and its magnetic composite: Batch and column study. Environmental Technology & Innovation, 23, 101685. https://doi.org/10.1016/j.eti.2021.101685
Karagöz, R., Tunali Akar, S., Turkyilmaz, S., Celik, S., & Akar, T. (2018). Process design and potential use of a regenerable biomagsorbent for effective decolorization process. Journal of the Taiwan Institute of Chemical Engineers, 93, 554–565. https://doi.org/10.1016/j.jtice.2018.09.001
Kobbing, J. F., Thevs, N., & Zerbe, S. (2013). The utilisation of reed (Phragmites australis): A review. Mires and Peat, 13, 1–14.
Kordialik-Bogacka, E. (2014). Saccharomyces pastorianus immobilized on brewer’s spent grain in continuous system for lead ion biosorption. International Biodeterioration & Biodegradation, 96, 191–197. https://doi.org/10.1016/j.ibiod.2014.09.018
Kousha, M., Daneshvar, E., Dopeikar, H., Taghavi, D., & Bhatnagar, A. (2012). Box-Behnken design optimization of Acid Black 1 dye biosorption by different brown macroalgae. Chemical Engineering Journal, 179, 158–168. https://doi.org/10.1016/j.cej.2011.10.073
Krueger, M. D., & d. S., Volkmann, A. C. & Rainert, K. T. (2019). Removal of textile dye Remazol Brilliant Blue Reactive (RBBR) using fibers of Citrullus lanatus (watermelon) and Cocos nucifera (green coconut) as adsorbent material, Revista Eletrônica em Gestão. Educação e Tecnologia Ambiental, 23, e5. https://doi.org/10.5902/2236117038526
Lagergren, S. (1898). Zur theorie der sogenannten adsorption gelöster stoffe. Kungliga Svenska Vetenskapsa-Kademiens, Handlingar, 24, 1–39.
Lan, D., Zhu, H., Zhang, J., Li, S., Chen, Q., Wang, C., Wu, T., & Xu, M. (2022). Adsorptive removal of organic dyes via porous materials for wastewater treatment in recent decades: A review on species, mechanisms and perspectives. Chemosphere, 293, 133464. https://doi.org/10.1016/j.chemosphere.2021.133464
Langmuir, I. (1918). The adsorption of gases on plane surfaces of glass, mica and platinum. Journal of the American Chemical Society, 40(9), 1361–1403. https://doi.org/10.1021/ja02242a004
Lenssen, J. P. M., Menting, F. B. J., van der Putten, W. H., & Blom, C. W. P. M. (1999). Effects of sediment type and water level on biomass production of wetland plant species. Aquatic Botany, 64(2), 151–165. https://doi.org/10.1016/S0304-3770(99)00012-1
Liao, J., Ding, L., Zhang, Y., & Zhu, W. (2022). Efficient removal of uranium from wastewater using pig manure biochar: Understanding adsorption and binding mechanisms. Journal of Hazardous Materials, 423, 127190. https://doi.org/10.1016/j.jhazmat.2021.127190
Liu, F., Chung, S., Oh, G., & Seo, T. S. (2012). Three-dimensional graphene oxide nanostructure for fast and efficient water-soluble dye removal. ACS Applied Materials & Interfaces, 4(2), 922–927. https://doi.org/10.1021/am201590z
Liu, X., Chen, Z.-Q., Han, B., Su, C.-L., Han, Q., & Chen, W.-Z. (2018). Biosorption of copper ions from aqueous solution using rape straw powders: Optimization, equilibrium and kinetic studies. Ecotoxicology and Environmental Safety, 150, 251–259. https://doi.org/10.1016/j.ecoenv.2017.12.042
Ma, C. M., Hong, G. B., & Wang, Y. K. (2020). Performance evaluation and optimization of dyes removal using rice bran-based magnetic composite adsorbent. Materials, 13(12), 2764. https://doi.org/10.3390/ma13122764
Maamoun, I., Eljamal, R., Falyouna, O., Bensaida, K., Sugihara, Y., & Eljamal, O. (2021). Insights into kinetics, isotherms and thermodynamics of phosphorus sorption onto nanoscale zero-valent iron. Journal of Molecular Liquids, 328, 115402. https://doi.org/10.1016/j.molliq.2021.115402
Osagie, C., Othmani, A., Ghosh, S., Malloum, A., Kashitarash Esfahani, Z., & Ahmadi, S. (2021). Dyes adsorption from aqueous media through the nanotechnology: A review. Journal of Materials Research and Technology, 14, 2195–2218. https://doi.org/10.1016/j.jmrt.2021.07.085
Özçelik, G., Bilgin, M., & Şahin, S. (2020). Carbamazepine sorption characteristics onto bentonite clay: Box-Behnken process design. Sustainable Chemistry and Pharmacy, 18, 100323. https://doi.org/10.1016/j.scp.2020.100323
Ravindiran, G., Gaddam, K., & Sunil, K. (2022). Removal of Reactive Red 120 in a batch technique using seaweed-based biochar: A response surface methodology approach. Journal of Nanomaterials, 2022, 3621807. https://doi.org/10.1155/2022/3621807
Ruan, W., Hu, J., Qi, J., Hou, Y., Zhou, C., & Wei, X. (2019). Removal of dyes from wastewater by nanomaterials: A review. Advanced Materials Letters, 10(1), 9–20. https://doi.org/10.5185/amlett.2019.2148
Sabbagh, N., Tahvildari, K., & Mehrdad Sharif, A. A. (2021). Application of chitosan-alginate bio composite for adsorption of malathion from wastewater: Characterization and response surface methodology. Journal of Contaminant Hydrology, 242, 103868. https://doi.org/10.1016/j.jconhyd.2021.103868
Sayin, F., Tunali Akar, S., Akar, T., Celik, S., & Gedikbey, T. (2021). Chitosan immobilization and Fe3O4 functionalization of olive pomace: An eco–friendly and recyclable Pb2+ biosorbent. Carbohydrate Polymers, 269, 118266. https://doi.org/10.1016/j.carbpol.2021.118266
Srivastava, A., Rani, R. M., Patle, D. S., & Kumar, S. (2022). Emerging bioremediation technologies for the treatment of textile wastewater containing synthetic dyes: A comprehensive review. Journal of Chemical Technology & Biotechnology, 97(1), 26–41. https://doi.org/10.1002/jctb.6891
Syafiuddin, A., & Fulazzaky, M. A. (2021). Decolorization kinetics and mass transfer mechanisms of Remazol Brilliant Blue R dye mediated by different fungi. Biotechnology Reports, 29, e00573. https://doi.org/10.1016/j.btre.2020.e00573
Tahir, S. S., & Rauf, N. (2006). Removal of a cationic dye from aqueous solutions by adsorption onto bentonite clay. Chemosphere, 63(11), 1842–1848. https://doi.org/10.1016/j.chemosphere.2005.10.033
Tunali Akar, S., Sayin, F., Turkyilmaz, S., & Akar, T. (2014). Multivariate optimization of the decolorization process by surface modified biomaterial: Box-Behnken design and mechanism analysis. Environmental Science and Pollution Research, 21(22), 13055–13068. https://doi.org/10.1007/s11356-014-3245-5
ul Haq, A., Saeed, M., Usman, M., Naqvi, S. A. R., Bokhari, T. H., Maqbool, T., Ghaus, H., Tahir, T. & Khalid, H. (2020). Sorption of chlorpyrifos onto zinc oxide nanoparticles impregnated pea peels (Pisum sativum L): Equilibrium, kinetic and thermodynamic studies. Environmental Technology & Innovation, 17, 100516. https://doi.org/10.1016/j.eti.2019.100516
Vijayaraghavan, K., & Balasubramanian, R. (2015). Is biosorption suitable for decontamination of metal-bearing wastewaters? A Critical Review on the State-of-the-Art of Biosorption Processes and Future Directions, Journal of Environmental Management, 160, 283–296. https://doi.org/10.1016/j.jenvman.2015.06.030
Vikrant, K., Giri, B. S., Raza, N., Roy, K., Kim, K.-H., Rai, B. N., & Singh, R. S. (2018). Recent advancements in bioremediation of dye: Current status and challenges. Bioresource Technology, 253, 355–367. https://doi.org/10.1016/j.biortech.2018.01.029
Yuan, D., Zhang, S., Tan, J., Dai, Y., Wang, Y., He, Y., Liu, Y., Zhao, X., Zhang, M., & Zhang, Q. (2020). Highly efficacious entrapment of Th (IV) and U (VI) from rare earth elements in concentrated nitric acid solution using a phosphonic acid functionalized porous organic polymer adsorbent. Separation and Purification Technology, 237, 116379. https://doi.org/10.1016/j.seppur.2019.116379
Zeldowitsch, J.: (1934). Uber den mechanismus der katalytischen oxydation von CO an MnO2, Acta Physicochimica U.R.S.S., 1, 364–449.
Zolgharnein, J., Shahmoradi, A., & Ghasemi, J. B. (2013). Comparative study of Box-Behnken, central composite, and Doehlert matrix for multivariate optimization of Pb (II) adsorption onto Robinia tree leaves. Journal of Chemometrics, 27(1–2), 12–20. https://doi.org/10.1002/cem.2487
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Sayin, F. Insight into Decolorization Characteristics of a Green Biocomposite Sorbent System Prepared by Immobilization of Fungal Cells on Lignocellulosic Matrix: Box-Behnken Design. Water Air Soil Pollut 233, 262 (2022). https://doi.org/10.1007/s11270-022-05721-7
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DOI: https://doi.org/10.1007/s11270-022-05721-7