Abstract
While foliar application of copper (Cu) containing fungicides can protect vines from fungal infections such as downy mildew (Plasmopara viticola), it can increase soil Cu content which increases disease susceptibility, especially in acidic soils. In this study, we hypothesized that lime (CaCO3 + MgCO3) addition may minimize Cu toxicity in vines by increasing pH and reducing Cu bioavailability. We applied two Cu (no Cu added, 50 mg Cu kg−1) and three lime (no lime added, 1.5, 3.0 Mg ha−1) quantities on a Typic Hapludalf soil. After 60 days, root, plant performance, and biochemistry were measured. As expected, high Cu content negatively affected growth, nutrient status, and metabolism of young vines. However, addition of 3.0 Mg ha−1 lime raised the soil pH from 4.5 to 6.1, decreasing Cu toxicity effects in young vines. Thus, lime addition is an effective strategy in protecting young vines in soils with high levels of Cu.
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Alaoui-Sossé, B., Genet, P., Vinit-Dunand, F., Toussaint, M.-L., Epron, D., & Badot, P.-M. (2004). Effect of copper on growth in cucumber plants (Cucumis sativus) and its relationships with carbohydrate accumulation and changes in ion contents. Plant Science, 166(5), 1213–1218. https://doi.org/10.1016/j.plantsci.2003.12.032.
Ambrosini, V. G., Rosa, D. J., Corredor Prado, J. P., Borghezan, M., Bastos de Melo, G. W., Fonsêca de Sousa Soares, C. R., et al. (2015). Reduction of copper phytotoxicity by liming: A study of the root anatomy of young vines (Vitis labrusca L.). Plant Physiology and Biochemistry, 96, 270–280. https://doi.org/10.1016/j.plaphy.2015.08.012.
Ambrosini, V. G., Rosa, D. J., Bastos de Melo, G. W., Zalamena, J., Cella, C., Simão, D. G., et al. (2018). High copper content in vineyard soils promotes modifications in photosynthetic parameters and morphological changes in the root system of ‘Red Niagara’ plantlets. Plant Physiology and Biochemistry, 128(1), 89–98. https://doi.org/10.1016/j.plaphy.2018.05.011.
Anderson, M. J., Gorley, R. N., & Clarke, K. R. (2008). PERMANOVA+ for PRIMER: Guide to Software and Statistical Methods. http://www.primer-e.com. Accessed 1 Aug 2019.
Bates, D., Maechler, M., Bolker, B., Walker, S. (2015). lme4: Linear Mixed-Effects Models Using Eigen and S4. R package version 1.1-10. https://www.CRAN.Rproject.org/package=lme4.
Brunetto, G., Miotto, A., Alberto Ceretta, C., Eugênio Schmitt, D., Heinzen, J., Pires de Moraes, M., et al. (2014). Archives of Agronomy and Soil Science Mobility of copper and zinc fractions in fungicide-amended vineyard sandy soils. Archives of Agronomy and Soil Science, 60(5), 609–624. https://doi.org/10.1080/03650340.2013.826348.
Cambrollé, J., García, J. L., Figueroa, M. E., & Cantos, M. (2015). Evaluating wild grapevine tolerance to copper toxicity. Chemosphere, 120, 171–178. https://doi.org/10.1016/j.chemosphere.2014.06.044.
Chaignon, V., & Hinsinger, P. (2003). A biotest for evaluating copper bioavailability to plants in a contaminated soil. Journal of Environmental Quality, 32(3), 824–833. https://doi.org/10.2134/jeq2003.8240.
Clarke, K.R. and Gorley, R.N. (2006) PRIMER v6: User Manual/Tutorial (Plymouth Routines in Multivariate Ecological Research). PRIMER-E, Plymouth.
Chen, P.-Y., Lee, Y.-I., Chen, B.-C., & Juang, K.-W. (2013). Effects of calcium on rhizotoxicity and the accumulation and translocation of copper by grapevines. Plant Physiology and Biochemistry, 73, 375–382. https://doi.org/10.1016/j.plaphy.2013.10.016.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(3), 350–356 https://pubs.acs.org/sharingguidelines. .
EMBRAPA. (1997). Manual de Métodos de Análise de Solo. Rio de Janeiro: EMBRAPA-CPNS https://www.agencia.cnptia.embrapa.br/Repositorio/Manual+de+Metodos_000fzvhotqk02wx5ok0q43a0ram31wtr.pdf. Accessed 1 August 2019.
Girotto, E., Ceretta, C. A., Brunetto, G., Miotto, A., Tiecher, T. L., De Conti, L., et al. (2014). Copper availability assessment of Cu-contaminated vineyard soils using black oat cultivation and chemical extractants. Environmental Monitoring and Assessment, 186(1), 9051–9063. https://doi.org/10.1007/s10661-014-4065-2.
Hoagland, D. R., & Arnon, D. I. (1950). The water-culture method for growing plants without soil: University of California. Circular. California Agricultural Experiment Station, 347(2nd edit), 32 pp.
Jones, J. B., Jr, Wolf, B., & Mills, H. A. (1991). Plant analysis handbook. A practical sampling, preparation, analysis, and interpretation guide. Plant analysis handbook. A practical sampling, preparation, analysis, and interpretation guide. https://www.cabdirect.org/cabdirect/abstract/19921969819. Accessed 1 August 2019.
Joris, H. A. W., da Fonseca, A. F., Asami, V. Y., Briedis, C., Borszowskei, P. R., & Garbuio, F. J. (2012). Adsorção de metais pesados após calagem superficial em um Latossolo Vermelho sob sistema de plantio direto. Revista Ciência Agronômica, 43(1), 1–10 www.ccarevista.ufc.br. Accessed 1 August 2019.
Juang, K.-W., Lee, Y.-I., Lai, H.-Y., & Chen, B.-C. (2014). Influence of magnesium on copper phytotoxicity to and accumulation and translocation in grapevines. Ecotoxicology and Environmental Safety, 104(1), 36–42. https://doi.org/10.1016/j.ecoenv.2014.02.008.
Kopittke, P. M., Kinraide, T. B., Wang, P., Blamey, F. P. C., Reichman, S. M., & Menzies, N. W. (2011). Alleviation of Cu and Pb rhizotoxicities in cowpea (Vigna unguiculata ) as related to ion activities at root-cell plasma membrane surface. Environmental Science & Technology, 45(11), 4966–4973. https://doi.org/10.1021/es1041404.
Length, R., Singmann, H., Love, J., Buerkner, P., Herve, M. (2018). Estimated Marginal Means, aka Least-Squares Means. R package version 1.3.0. https://www.mran.microsoft.com/snapshot/2018-03-30/web/packages/emmeans/emmeans.pdf.
Mccready, R. M., Guggolz, J., Silviera, V., & Owens, H. S. (1950). Determination of starch and amylose in vegetables. Analytical Chemistry, 22(9), 1156–1158 https://pubs.acs.org/sharingguidelines. Accessed 1 August 2019.
Meharg, A. A. (2011). Trace elements in soils and plants. 4th edition. By A. Kabata-Pendias. Experimental Agriculture, 47(4), 739–739. https://doi.org/10.1017/S0014479711000743.
Novozamsky, I., Lexmond, T. M., & Houba, V. J. G. (1993). A single extraction procedure of soil for evaluation of uptake of some heavy metals by plants. International Journal of Environmental Analytical Chemistry, 51(1–4), 47–58. https://doi.org/10.1080/03067319308027610.
Printz, B., Lutts, S., Hausman, J.-F., & Sergeant, K. (2016). Copper trafficking in plants and its implication on cell wall dynamics. Frontiers in Plant Science, 7, 601. https://doi.org/10.3389/fpls.2016.00601.
Read, S. M., & Northcote, D. H. (1981). Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Analytical Biochemistry, 116(1), 53–64. https://doi.org/10.1016/0003-2697(81)90321-3.
Rodrigo-Moreno, A., Andrés-Colás, N., Poschenrieder, C., Gunsé, B., Peñarrubia, L., & Shabala, S. (2013). Calcium- and potassium-permeable plasma membrane transporters are activated by copper in Arabidopsis root tips: linking copper transport with cytosolic hydroxyl radical production. Plant, Cell & Environment, 36(4), 844–855. https://doi.org/10.1111/pce.12020.
Rosa, D. J., Ambrosini, V. G., Basso, A., Borghezan, M., Bruneto, G., & Pescador, R. (2014). Photosynthesis and growth of young “Niágara Branca” vines (Vitis labrusca L.) cultivated in soil with high levels of copper and liming. BIO Web of Conferences, 3, 01005. https://doi.org/10.1051/bioconf/20140301005.
Tedesco, M., Gianello, C., & Bissani, C. (1995). Análises de solo, plantas e outros materiais. UFRGS.
Toselli, M., Baldi, E., Marcolini, G., Malaguti, D., Quartieri, M., Sorrenti, G., & Marangoni, B. (2009). Response of potted grapevines to increasing soil copper concentration. Australian Journal of Grape and Wine Research, 15(1), 85–92. https://doi.org/10.1111/j.1755-0238.2008.00040.x.
Trentin, E., Facco, D. B., Hammerschmitt, R. K., Avelar Ferreira, P. A., Morsch, L., Belles, S. W., et al. (2019). Potential of vermicompost and limestone in reducing copper toxicity in young grapevines grown in Cu-contaminated vineyard soil. Chemosphere, 226, 421–430. https://doi.org/10.1016/j.chemosphere.2019.03.141.
Yruela, I. (2009). Copper in plants: acquisition, transport and interactions. Functional Plant Biology, 36(5), 409–430. https://doi.org/10.1071/FP08288.
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This study was performed thankfully to the funding granted by Coordination of Superior Level Staff Improvement (CAPES–Brazil).
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Rosa, D.J., Ambrosini, V.G., Kokkoris, V. et al. Lime Protection for Young Vines Exposed to Copper Toxicity. Water Air Soil Pollut 231, 296 (2020). https://doi.org/10.1007/s11270-020-04662-3
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DOI: https://doi.org/10.1007/s11270-020-04662-3