Abstract
Microorganisms are frequently exposed to flowing fluid, thus to investigate bacterial characteristics under different hydrodynamic conditions is of great importance in microbial ecology. This study characterized bacterial growth and phenol biodegradation of three strains, i.e., Microbacterium oxydans (rod-shaped, non-motile), Alcaligenes faecalis (rod-shaped, motile), and Staphylococcus haemolyticus (spherical, non-motile) in shake-flask cultures at various rotating speeds. For all the strains, a higher rotating speed always resulted in a shorter lag phase, indicating that the strains showed a superior adaptability under higher hydrodynamic conditions. The maximum specific growth rate of M. oxydans, A. faecalis, and S. haemolyticus increased rapidly with the increase of energy dissipation rate till the highest value of 0.386, 0.240, and 0.323 1/h and then decreased as the rotating speed further increased. The phenol biodegradation rate was also dependent on rotating speed, and the trends were consistent with the growth rate variations. A predictive model similar to Haldane model was proposed and was fitted well (R2 > 0.913) with bacterial growth under different hydrodynamic conditions. According to the predictive model, the optimum hydrodynamic conditions for the growth of M. oxydans, A. faecalis, and S. haemolyticus were 3.099, 2.197, and 2.289 m2/s3, respectively. The results suggested that non-motile and rod-shaped bacteria were more dependent on hydrodynamic conditions than motile and spherical ones, which could be attributed to the discrepancies in bacterial morphology and motility. The results provide a better understanding on bacterial responses to various hydrodynamic conditions and could be further applied in the bioremediation of contaminated water.
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References
Al-Homoud, A., Hondzo, M., & LaPara, T. (2007). Fluid dynamics impact on bacterial physiology: biochemical oxygen demand. Journal Of Environmental Engineering-Asce, 133(2), 226–236. https://doi.org/10.1061/(asce)0733-9372(2007)133:2(226)
Berdalet, E., Peters, F., Koumandou, V. L., Roldan, C., Guadayol, O., & Estrada, M. (2007). Species-specific physiological response of dinoflagellates to quantified small-scale turbulence. Journal of Phycology, 43(5), 965–977. https://doi.org/10.1111/j.1529-8817.2007.00392.x
Brown, P. J. B., Kysela, D. T., & Brun, Y. V. (2011). Polarity and the diversity of growth mechanisms in bacteria. Seminars in Cell & Developmental Biology, 22(8), 790–798. https://doi.org/10.1016/j.semcdb.2011.06.006
Chaban, B., Hughes, H. V., & Beeby, M. (2015). The flagellum in bacterial pathogens: for motility and a whole lot more. Semin Cell Dev Biol, 46, 91–103. https://doi.org/10.1016/j.semcdb.2015.10.032
Chengala, A. A., Hondzo, M., Troolin, D., & Lefebvre, P. A. (2010). Kinetic responses of Dunaliella in moving fluids. Biotechnology and Bioengineering, 107(1), 65–75. https://doi.org/10.1002/bit.22774
Djokic, L., Narancic, T., Nikodinovic-Runic, J., Savic, M., & Vasiljevic, B. (2011). Isolation and characterization of four novel gram-positive bacteria associated with the rhizosphere of two endemorelict plants capable of degrading a broad range of aromatic substrates. Applied Microbiology and Biotechnology, 91(4), 1227–1238. https://doi.org/10.1007/s00253-011-3426-9
Garcia-Ochoa, F., Gomez, E., Alcon, A., & Santos, V. E. (2013). The effect of hydrodynamic stress on the growth of Xanthomonas campestris cultures in a stirred and sparged tank bioreactor. Bioprocess and Biosystems Engineering, 36(7), 911–925. https://doi.org/10.1007/s00449-012-0825-y
Guasto, J. S., Rusconi, R., & Stocker, R. (2012). Fluid mechanics of planktonic microorganisms. In S. H. Davis & P. Moin (Eds.), Annual Review Of Fluid Mechanics (Vol. 44, pp. 373–400).
Hondzo, M., & Wüest, A. (2009). Do microscopic organisms feel turbulent flows? Environmental Science & Technology, 43(3), 764–768. https://doi.org/10.1021/es801655p
Jiang, L. C., Ruan, Q. P., Li, R. L., & Li, T. D. (2013). Biodegradation of phenol by using free and immobilized cells of Acinetobacter sp BS8Y. Journal of Basic Microbiology, 53(3), 224–230. https://doi.org/10.1002/jobm.201100460
Kaya, T., & Koser, H. (2012). Direct upstream motility in Escherichia Coli. Biophysical Journal, 102(7), 1514–1523. https://doi.org/10.1016/j.bpj.2012.03.001
Kiørboe, T. (1993). Turbulence, phytoplankton cell size, and the structure of pelagic food webs. Advances in Marine Biology, 29, 1–72.
Linek, V. (1981). Chemical engineering use of catalyzed sulphite oxidation kinetics for the determination of mass transfer characteristics of gas liquid contractors. Chemical Engineering Science, 36(11), 1747–1768. https://doi.org/10.1016/0009-2509(81)80124-8
Liu, Y.-S., Wu, J.-Y., & Ho, K.-p. (2006). Characterization of oxygen transfer conditions and their effects on Phaffia rhodozyma growth and carotenoid production in shake-flask cultures. Biochemical Engineering Journal, 27(3), 331–335. https://doi.org/10.1016/j.bej.2005.08.031
Malits, A., Peters, F., Bayer-Giraldi, M., Marrase, C., Zoppini, A., Guadayol, O., & Alcaraz, M. (2004). Effects of small-scale turbulence on bacteria: a matter of size. Microbial Ecology, 48, 287–299. https://doi.org/10.1007/s00248-004-0133-4
Mitrovic, S. M., Hardwick, L., & Dorani, F. (2011). Use of flow management to mitigate cyanobacterial blooms in the Lower Darling River, Australia. Journal of Plankton Research, 33(2), 229–241. https://doi.org/10.1093/plankt/fbq094
Paisio, C. E., Talano, M. A., González, P. S., Busto, V. D., Talou, J. R., & Agostini, E. (2012). Isolation and characterization of a Rhodococcus strain with phenol-degrading ability and its potential use for tannery effluent biotreatment. Environmental Science and Pollution Research, 19(8), 3430–3439. https://doi.org/10.1007/s11356-012-0870-8
Pankratov, T. A., Ivanova, A. O., Dedysh, S. N., & Liesack, W. (2011). Bacterial populations and environmental factors controlling cellulose degradation in an acidic sphagnum peat. Environmental Microbiology, 13(7), 1800–1814. https://doi.org/10.1111/j.1462-2920.2011.02491.x
Peter, C. P., Suzuki, Y., & Buchs, J. (2006). Hydromechanical stress in shake flasks: correlation for the maximum local energy dissipation rate. Biotechnology and Bioengineering, 93(6), 1164–1176. https://doi.org/10.1002/bit.20827
Peters, F., Arin, L., Marrasé, C., Berdalet, E., & Sala, M. M. (2006). Effects of small-scale turbulence on the growth of two diatoms of different size in a phosphorus-limited medium. Journal of Marine Systems, 61(3–4), 134–148. https://doi.org/10.1016/j.jmarsys.2005.11.012
Peters, F., Marrase, C., Gasol, J. M., Sala, M. M., & Arin, L. (1998). Effects of turbulence on bacterial growth mediated through food web interactions. Marine Ecology Progress Series, 172, 293–303. https://doi.org/10.3354/meps172293
Polymenakou, P. N., & Stephanou, E. G. (2005). Effect of temperature and additional carbon sources on phenol degradation by an indigenous soil pseudomonad. Biodegradation, 16(5), 403–413. https://doi.org/10.1007/s10532-004-3333-1
Randich, A. M., & Brun, Y. V. (2015). Molecular mechanisms for the evolution of bacterial morphologies and growth modes. Frontiers in Microbiology, 6. https://doi.org/10.3389/fmicb.2015.00580
Rusconi, R., & Stocker, R. (2015). Microbes in flow. Current Opinion in Microbiology, 25, 1–8. https://doi.org/10.1016/j.mib.2015.03.003
Sharma, B. M., Bharat, G. K., Tayal, S., Nizzetto, L., Čupr, P., & Larssen, T. (2014). Environment and human exposure to persistent organic pollutants (POPs) in India: a systematic review of recent and historical data. Environment International, 66, 48–64. https://doi.org/10.1016/j.envint.2014.01.022
van Dillewijn, P., Caballero, A., Paz, J. A., González-Pérez, M. M., Oliva, J. M., & Ramos, J. L. (2007). Bioremediation of 2,4,6-trinitrotoluene under field conditions. Environmental Science & Technology, 41(4), 1378–1383. https://doi.org/10.1021/es062165z
Wang, L., Li, Y., Zhang, W., Niu, L., Du, J., Cai, W., & Wang, J. (2016a). Isolation and characterization of two novel psychrotrophic decabromodiphenyl ether-degrading bacteria from river sediments. Environmental Science and Pollution Research, 23(11), 10371–10381. https://doi.org/10.1007/s11356-015-5660-7
Wang, L. Q., Li, Y., Niu, L. H., Dai, Y., Wu, Y., & Wang, Q. (2016b). Isolation and growth kinetics of a novel phenol-degrading bacterium microbacterium oxydans from the sediment of Taihu Lake (China). Water Science and Technology, 73(8), 1882–1890. https://doi.org/10.2166/wst.2016.036
Yang, R. D., & Humphrey, A. E. (1975). Dynamic and steady state studies of phenol biodegradation in pure and mixed cultures. Biotechnology and Bioengineering, 17(8), 1211–1235. https://doi.org/10.1002/bit.260170809
Young, K. D. (2007). Bacterial morphology: why have different shapes? Current Opinion in Microbiology, 10, 596–600. https://doi.org/10.1016/j.mib.2007.09.009
Funding
This study was financially supported by National Natural Science Foundation of China (51779076 and 91547105), Foundation for Innovative Research Groups of the National Natural Science Foundation of China (51421006), Fundamental Research Funds for the Central Universities (2016B10614), PAPD and TAPP (PPZY2015A051), National Undergraduate Training Program for Innovation and Entrepreneurship (201510294029).
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Yang, N., Li, Y., Wang, L. et al. Hydrodynamic Conditions Influence Bacterial Growth and Phenol Biodegradation of Strains with Different Morphology and Motility. Water Air Soil Pollut 229, 93 (2018). https://doi.org/10.1007/s11270-018-3695-3
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DOI: https://doi.org/10.1007/s11270-018-3695-3