Abstract
The intensive pig production has been causing huge amounts of pig slurry with high content of potential pollutants. However, there is a lack of information on the efficiency of combined techniques applied to pig slurry purification. The objective of this research was to assess the pollutant removal efficiency and pathogenic microorganism decrease using mechanical treatments, phytoextraction, and microalgae bioremediation. The purification system was located in the southeast of Spain. Physico-chemical and microbiological parameters were studied in each module of treatment. We observed significant declines for total suspended solids (89 %), settleable solids (100 %), chemical oxygen demand (91 %), biochemical oxygen demand (90 %), total phosphorus (97 %), copper (96 %), zinc (92 %), total nitrogen (89 %), total coliforms (78 %), fecal coliforms (70 %), fecal streptococcus (75 %), Salmonella, Shigella, and Escherichia coli (100 %) in the final effluent of the combined purification system. This survey pointed out the effectiveness of phytoextraction and bioremediation treatments. The results indicated the high efficiency of the purification system, minimizing environmental and human risks.
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References
AEMET. Agencia Estatal de Meteorología. Gobierno de España. (2012). In: http://www.aemet.es/ . Updated: April 2014.
Antoniou, P., Hamilton, J., Koopman, B., Jain, R., Holloway, B., Lyberatos, G., & Svoronos, S. A. (1990). Effect of temperature and pH on the effective maximum specific growth rate of nitrifying bacteria. Water Research, 24, 97–101.
APHA, AWWA, WEF. (2012). Standard methods for examination of water and wastewater. 22nd American Public Health Association (Eds.). Washington. 1360 pp. In: http://www.standardmethods.org. Updated: May 2014.
Ayers, R. S., & Wescot, D. W. (1984). Water quality for agriculture. Irrigation and drainage paper 29. Roma: FAO.
Bock, E., Koops, H., Harms, H. (1986). Cell biology of nitrifying bacteria. In Prosser, J. I., (ed.) Nitrification, IRL Press. pp. 17–38.
Bonmati, A., & Flotats, X. (2003). Air stripping of ammonia from pig slurry: characterization and feasibility as a pre- or post-treatment to mesophilic anaerobic digestion. Waste Management, 23, 261–272.
Brix, H. (1997). Do macrophytes play a role in constructed treatment wetlands? Water Science and Technology, 35, 11–17.
Burton, C. H. (1997). Manure management—treatment strategies for sustainable agriculture. Silsoe: Silsoe Research Institute.
Burton, C. H. (2007). The potential contribution of separation technologies to the management of livestock manure. Livestock Science, 112, 208–216.
Caballero-Lajarín, A., Faz Cano, A., Lobera Lössel, J. B. (2012). Humedal artificial y uso del mismo para la fitopurificación de efluentes líquidos. Patent: ES 2 363 363 B2.
Carpenter, S. R., Caraco, N. F., Correll, D. L., Howarth, R. W., Sharpley, A. N., & Smith, V. H. (1998). Nonpoint pollution of surface waters with phosphorus and nitrogen. Ecological Applications, 8, 559–568.
Christos, S. A., & Tsihrintzis, V. A. (2007). Effect of temperature, HRT, vegetation and porous media on removal efficiency of pilot-scale horizontal subsurface flow constructed wetlands. Ecological Engineering, 29, 173–191.
de Godos, I., Blanco, S., García-Encina, P. A., Becares, E., & Muñoz, R. (2009). Long-term operation of high rate algal ponds for the bioremediation of piggery wastewaters at high loading rates. Bioresource Technology, 100(19), 4332–4339.
de Godos, I., Vargas, V. A., Blanco, S., García González, M. C., Soto, R., García-Encina, P. A., Becares, E., & Muñoz, R. A. (2010). Comparative evaluation of microalgae for the degradation of piggery wastewater under photosynthetic oxygenation. Bioresource Technology, 101, 5150–5158.
de la Noue, J., & Basseres, A. (1989). Biotreatment of anaerobically digested swine manure with microalgae. Biological Wastes, 29, 17–31.
DIN 38 409 - H41 -1 and DIN ISO 15705 –H45. German standard methods for the examination of water, wastewater and sludge.
Drizo, A., Frost, C. A., Grace, J., & Smith, K. A. (1999). Physico-chemical screening of phosphate-removing substrates for use in constructed wetland systems. Water Research, 33, 3595–3602.
Duchaufour, P. H. (1970). Precis de Pedologie. Masson. Paris. 481 pp.
Finlayson, C. M., & Chick, A. J. (1983). Testing the potential of aquatic plants to treat abattoir effluent. Water Research, 17, 415–22.
Ge, Y., Han, W., Huang, C., Wang, H., Liu, D., Chang, S. X., Gu, B., Zhang, C., Gu, B., Fan, X., Du, Y., & Chang, J. (2015). Positive effects of plant diversity on nitrogen removal in microcosms of constructed wetlands with high ammonium loading. Ecological Engineering, 82, 614–623.
Gómez-Garrido, M., Martínez-Martínez, S., Faz-Cano, A., Büyükkılıç-Yanardag, A., & Arocena, J. M. (2014). Soil fertility status and nutrients provided to spring barley (Hordeum distichon L.) by pig slurry. Chilean Journal of Agricultural Research, 74(1), 73–82.
Haberl, R., Perfler, R., & Mayer, H. (1995). Constructed wetlands in Europe. Water Science and Technology, 32, 305–315.
Healy, M. G., & O’ Flynn, C. J. (2011). The performance of constructed wetlands treating primary, secondary and dairy soiled water in Ireland (a review). Journal of Environmental Management, 92(10), 2348–2354.
Hiley, P. (2003). Performance of wastewater treatment and nutrient removal wetlands. In U. Mander & P. Jenssen (Eds.), Constructed wetlands for wastewater treatment in cold climates (reedbeds) in cold temperature climates (pp. 1–18). Southampton: WIT Press.
Hill, V. R., Pasternak, J. I., Rice, J. M., Marra, M. C., Humenik, F. J., Sobsey, M. D., Szogi, A. A., & Hunt, P. G. (1999). Economics of nitrogen and enteric microbe reductions for alternative swine waste treatment techniques. In G. B. Havenstein (Ed.), Proceedings of the animal waste management symposium (pp. 297–301). Raleigh: NCSU Animal Waste Management Field Day Committee.
Hoffman, J. P. (1998). Wastewater treatment with suspended and nonsuspended algae. Journal of Phycology, 34, 757–763.
Huang, J., Reneau, R. B., Jr., & Hagedorn, C. (2000). Nitrogen removal in constructed wetlands employed to treat domestic wastewater. Water Research, 34(9), 2582–2588.
Hunt, P. G., Szogi, A. A., Humenik, F. J., Rice, J. M., Matheny, T. A., & Stone, K. C. (2002). Constructed wetlands for treatment of swine wastewaters from an anaerobic lagoon. Transactions of ASAE, 45, 639–647.
Kadlec, R. (1992). Hydrological factors in wetland water treatment. In D. A. Hammer (Ed.), Constructed wetland for wastewater treatment: municipal, industrial and agricultural (pp. 25–29). Chelsea: Lewis Publishers.
Kadlec, R., & Knight, R. L. (1996). Treatment wetlands. Boca Raton: Lewis Publishers.
Karathanasis, A. D., & Thompson, Y. L. (1993). Substrate effects on metal retention and speciation in simulated acid mine wetlands. Bulletin of Environmental Contamination and Toxicology, 51, 421–429.
Kebede-Wheshead, E., Pizarro, E., & Mulbry, W. W. (2003). Environmental and economic aspects of recycling livestock wastes-algae production using waste products. Southern Journal of Agricultural Economics, 3, 1275–1282.
Knight, R. L., Payne, V. W. E., Jr., Borer, R. E., Clarke, R. A., Jr., & Pries, J. H. (2000). Constructed wetlands for livestock wastewater management. Ecological Engineering, 15, 41–55.
Knops, J. M. H., Bradley, K. L., & Wedin, D. A. (2002). Mechanisms of plant species impacts on ecosystem nitrogen cycling. Ecology Letters, 5(3), 454–46.
Krapaca, I. G., Deya, W. S., Roya, W. R., Smythb, C. A., Stormentc, E., & Sargenta, S. L. (2002). Impacts of swine manure pits on groundwater quality. Environmental Pollution, 120, 475–492.
Lee, B. H., & Scholz, M. (2007). What is the role of Phragmites australis in experimental constructed wetland filters treating urban runoff? Ecological Engineering, 29(1), 87–95.
Lema, E., Machunda, R., Nicholas, NJAU. (2014). Influence of macrophyte types towards agrochemical phytoremediation in a tropical environment. International Journal of Engineering Research and General Science 2(5).
Carrasco, M. L. (2005). Tesis doctoral: Utilización agronómica de purines de cerdo en brócoli y sandía en condiciones mediterráneas semiáridas. Influencia en el sistema suelo- planta. Pp. 374.
Lorimor, J., Fulhage, C., Zhang, R., Funk, T., Sheffield, R., Sheppard, D. C., Newton, G.L. (2006). Manure management strategies and technologies. In: Animal agriculture and the environment: National Center for Manure and Animal Waste Management white papers. American Society of Agricultural and Biological Engineers, St. Joseph, MI, USA.
Macherey-Nagel GmbH & Co. KG. Web: http://www.mn-net.com. Nanocolor Test; ref: 985 028/29, 985 055, 985 064.
Marchand, L., Mench, M., Jacob, D. L., & Ottem, M. L. (2010). Metal and metalloid removal in constructed wetlands, with emphasis on the importance of plants and standardized measurements: a review. Environmental Pollution, 158, 3447–3461.
Mashauri, D. A., Mulungu, D. M. M., & Abdulhussein, B. S. (2000). Constructed wetland at the University of Dar Es Salaam. Water Research, 34, 1135–1144.
Massé, D., Gilbert, Y., & Topp, E. (2011). Pathogen removal in farm-scale psychrophilic anaerobic digesters processing swine manure. Bioresource Technology, 102, 641–646.
Melse, R. W., & Verdoes, N. (2005). Evaluation of four farm scale systems for the treatment of liquid pig manure. Biosystems Engineering, 92, 47–57.
Molina-Grima, E., (1999). Microalgae mass culture methods. In: Flickinger, M. C., Drew, S.W. (Eds.), Encyclopedia of bioprocess technology: fermentation, biocatalysis and bioseparation. Wiley.
Møller, H. B., Lund, I., & Sommer, S. G. (2000). Solid–liquid separation of livestock slurry: efficiency and cost. Bioresource Technology, 74(3), 223–229.
Monroy, F., Aira, M., & Domínguez, J. (2009). Reduction of total coliform numbers during vermicomposting is caused by short-term direct effects of earthworms on microorganisms and depends on the dose of application of pig slurry. Science of the Total Environment, 407, 5411–5416.
Moral, R., Pérez-Murcia, M. D., Pérez-Espinosa, A., Moreno-Caselles, J., & Paredes, C. (2005). Estimation of nutrient values of pig slurries in southeast Spain using easily determined properties. Waste Management, 25, 719–725.
Mulbry, W., Kebede Westhead, E., Pizarro, C., & Sikora, L. (2005). Recycling of manure nutrients: use of algal biomass from dairy manure treatment as a slow release fertilizer. Bioresource Technology, 96(4), 451–458.
Muñoz, R., & Guieysse, B. (2006). Algal-bacteria processes for the treatment of hazardous contaminants, a review. Water Research, 40(2006), 2799–2815.
Neralla, S., Weaver, R. W., Lesikar, B. J., & Persyn, R. A. (2000). Improvement of domestic wastewater quality by subsurface flow constructed wetlands. Bioresource Technology, 75, 19–25.
Oswald, W. J., Gotaas, H. B., & Golueke, C. G. (1957). Algae in wastewater treatment. Sewage and Industrial Wastes, 29, 437–455.
Phillips, V. R., Scholtens, R., Lee, D. S., Garland, J. A., & Sneath, R. W. (2000). A review of methods for measuring emission rates of ammonia from livestock buildings and slurry or manure stores. Part 1: assessment of basic approaches. Journal of Agricultural Engineering Research, 77, 355–364.
Plaza, C. (2002). Aprovechamiento agrícola del purín de cerdo en agroecosistemas semiáridos: efectos sobre suelos y plantas. Tesis Doctoral. Universidad Autónoma de Madrid.
Plaza, C., Hernández, D., García-Gil, J., & Polo, A. (2004). Microbial activity in pig slurry-amended soils under semiarid conditions. Soil Biology and Biochemistry, 36, 1577–1585.
Potter, C. L. & Karathanasis, A. D. (2001). Vegetation effects on the performance of constructed wetlands treating domestic wastewater. Proceedings of Ninth National Symposium on Individual and Small Community Sewage Systems, ASAE, Fort Worth, TX, March 2001, pp. 663–673.
Puigagut, J., Vilaseñor, J., Salas, J. J., Béceras, E., & García, J. (2007). Subsurface flow constructed wetlands in Spain for the sanitation of small communities: a comparative study. Ecological Engineering, 30, 312–9.
Reddy, K. R., & Patrick, W. H. (1984). Nitrogen transformations and loss in flooded soils and sediments. Critical Review in Environmental Control, 13, 273–309.
Reddy, K. R., Patrick, W. H., & Lindau, C. W. (1989). Nitrification–denitrification at the plant root-sediment interface in wetlands. Limnology and Oceanography, 34, 1004–1013.
Reimann, W., & Potsdam, M. S. (1991). Fest-flussig-trennung anaerob behandelter gulle. Landtechnik, 46, 11–91.
Ros, M., García, C., & Hernández, T. (2006). A full-scale study of treatment of pig slurry by composting: kinetic changes in chemical and microbial properties. Waste Management, 26, 1108–1118.
Rufete, B., Pérez-Murcia, M. D., Pérez-Espinosa, A., Moral, R., Moreno-Caselles, J., & Paredes, C. (2006). Total and faecal coliform bacteria persistence in a pig slurry amended soil. Livestock Science, 102, 211–215.
Sánchez, M., & González, J. L. (2005). The fertilizer value of pig slurry. I. Values depending on the type of operation. Bioresource Technology, 96, 1117–1123.
Scholz, M. (2006). Wetland systems to control urban runoff. Amsterdam: Elsevier.
Sheoran, A. S., & Sheoran, V. (2006). Heavy metal removal mechanism of acid mine drainage in wetlands: a critical review. Minerals Engineering, 19(2), 105–116.
Sorensen, P., & Thomsen, I. K. (2005). Separation of pig slurry and plant utilization and loss of nitrogen-15-labeled slurry nitrogen. Soil Science Society of America Journal, 69, 1644–1651.
Steer, D., Fraser, D. L., Boddy, J., & Seibert, B. (2002). Efficiency of small constructed wetlands for subsurface treatment of single-family domestic effluent. Ecological Engineering, 18, 429–440.
Suresh, A., Choi, H. L., Ohb, D. I., & Moon, O. K. (2009). Prediction of the nutrients value and biochemical characteristics of swine slurry by measurement of EC—electrical conductivity. Bioresource Technology, 100, 4683–4689.
Tofant, A., Vucˇemilo, M., Pavicˇic´, Z., & Milic´, D. (2006). The hydrogen peroxide, as a potentially useful slurry disinfectant. Livestock Science, 102, 243–247.
Vymazal, J. (2002). The use of sub-surface constructed wetlands for wastewater treatment in the Czech Republic: 10 years experience. Ecological Engineering, 18, 633–646.
Vymazal, J. (2011). Plants used in constructed wetlands with horizontal subsurface flow: a review. Hydrobiology, 674(1), 133–156.
Vymazal, J., Brix, H., Cooper, P. F., Haberl, R., Perfler, R., Laber, J. (1998). Removal mechanisms and types of constructed wetlands. In: constructed wetlands for waste-water treatment in Europe. Vymazal, J., Brix, H., Cooper, P., Green, M. B., Haberl, R. (Eds.), Leiden, pp 17–66.
Walker, P. M., Wade, C. A., & Kelley, T. R. (2010). Evaluation of a polyacrylamide assisted solid/liquid separation system for the treatment of liquid pig manure. Biosystems Engineering, 105, 241–246.
Werblan, D., Smith, R. J., Van der Valk, A. G., Davis, C. B. (1978). Treatment of waste from a confined hog feeding unit by using artificial marshes. In: Mickim, H. L., (Ed.), Proceedings of international symposium on land treatment of wastewater. Hannover, New Hampshire, USA, pp. 1–13.
Willers, H. C., Derikx, P. J. L., ten Have, P. J. W., & Vijn, T. K. (1998). Nitrification limitation in animal slurries at high temperatures. Bioresource Technology, 64, 47–54.
Wong, J. W. C., & Selvam, A. (2009). Reduction of indicator and pathogenic microorganisms in pig manure through fly ash and lime addition during alkaline stabilization. Journal of Hazardous Materials, 169, 882–889.
Zhang, R. H., & Westerman, P. W. (1997). Solid-liquid separation of animal manure for odor control and nutrient management. Applied Engineering in Agriculture, 13, 657–664.
Zimno, O. R., van der Steen, N. P., & Gijzen, H. J. (2003). Comparison of ammonia volatilisation rates in algae and duckweed-based waste stabilisation ponds treating domestic wastewater. Water Research, 37, 4587–4594.
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This research was possible thanks to the financial support from the Spanish Ministry of Education and Science, Projects PET2006 - 0075 and CTM 2007-65888, and the Department of Agriculture and Water, Government of Murcia Region.
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Caballero-Lajarín, A., Zornoza, R., Faz, A. et al. Combination of Low-Cost Technologies for Pig Slurry Purification Under Semiarid Mediterranean Conditions. Water Air Soil Pollut 226, 341 (2015). https://doi.org/10.1007/s11270-015-2606-0
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DOI: https://doi.org/10.1007/s11270-015-2606-0