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Molecular evolution of GII.P17-GII.17 norovirus associated with sporadic acute gastroenteritis cases during 2013–2018 in Zhoushan Islands, China

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Abstract

In this study, we investigated the molecular characteristics and spatio-temporal dynamics of GII.P17-GII.17 norovirus in Zhoushan Islands during 2013–2018. We collected 1849 samples from sporadic acute gastroenteritis patients between January 2013 and August 2018 in Zhoushan Islands, China. Among the 1849 samples, 134 (7.24%) samples were positive for human norovirus (HuNoV). The complete sequence of GII.17 VP1 gene was amplified from 31 HuNoV-positive samples and sequenced. A phylogenetic tree was constructed based on the full-length sequence of the VP1 gene. Phylogenetic analysis revealed that the GII.17 genotype detected during 2014–2018 belongs to the new GII.17 Kawasaki variant. Divergence analysis revealed that the time of the most recent common ancestor (TMRCA) of GII.17 in Zhoushan Islands was estimated to be between 1997 and 1998. The evolutionary rate of the VP1 gene of the GII.17 genotype norovirus was 1.14 × 10–3 (95% HPD: 0.62–1.73 × 10–3) nucleotide substitutions/site/year. The spatio-temporal diffusion analysis of the GII.17 genotype identified Hong Kong as the epicenter for GII.17 dissemination. The VP1 gene sequence of Zhoushan Island isolates correlated with that of Hong Kong and Japan isolates.

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Acknowledgements

We are thankful to Dr. Xingguang Li for his suggestion to improve this work.

Funding

This work was supported by the Zhoushan Medical and Health Science Technology Project, Zhoushan, China [(2017G03), (2014C31073)] and special fund project of graduate student innovation in Jiangxi Province, China (YC2017-S093).

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Author notes

  1. Can Chen and Bing Wu have contributed equally to this work.

Authors and Affiliations

  1. Zhoushan Center for Disease Control and Prevention, Zhoushan, Zhejiang Province, China

    Can Chen, Bing Wu, Hui Zhang, Ke-Feng Li, Hong-Ling Wang & Jian-Bo Yan

  2. Zhejiang Provincial Key Laboratory of Health Risk Factors for Seafood, Zhoushan Center for Disease Control and Prevention, Zhoushan, Zhejiang Province, China

    Can Chen, Bing Wu, Hui Zhang, Ke-Feng Li, Hong-Ling Wang & Jian-Bo Yan

  3. Jiangxi Province Key Laboratory of Preventive Medicine, School of Public Health, Nanchang University, Nanchang, 330006, Jiangxi, China

    Can Chen, Rong Liu & Jian-Bo Yan

  4. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affifiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China

    Can Chen

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  1. Can Chen
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Contributions

CC, BW, and HZ performed the experiments and analyzed the data. CC and BW prepared the manuscript. RL and KFL edited the manuscript. HLW and JBY designed the study and provided funding. All authors read and approved the final manuscript.

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Correspondence to Hong-Ling Wang or Jian-Bo Yan.

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Table S1. List of the VP1 sequences and the respective strains used in this study. (DOC 138 kb)

11262_2020_1744_MOESM2_ESM.doc

TableS2. Genotype distribution of human norovirus (HuNoV) strains detected in Zhoushan Islands between 2013 and 2018. (DOC 46 kb)

Table S3. Sequences submitted to Genbank. (DOC 51 kb)

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Chen, C., Wu, B., Zhang, H. et al. Molecular evolution of GII.P17-GII.17 norovirus associated with sporadic acute gastroenteritis cases during 2013–2018 in Zhoushan Islands, China. Virus Genes 56, 279–287 (2020). https://doi.org/10.1007/s11262-020-01744-6

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