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Human papillomavirus type 8 E7 protein binds nuclear myosin 1c and downregulates the expression of pre-rRNA

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Abstract

Our aim was to search for new cellular binding partners for the E6 and E7 oncogenes of beta human papillomaviruses (HPV), whose direct role in skin carcinogenesis has not been thoroughly investigated. By employing glutathione S-transferase pulldown and coimmunoprecipitation, we identified nuclear myosin 1c as a binding partner of HPV 8 E7 protein. As nuclear myosin 1c is an essential component of the RNA polymerase I transcription complex, we studied the effects of HPV 8 E7 protein expression on ribosomal RNA (rRNA) expression. Here we show that the activity of RNA polymerase I is decreased and that pre-rRNA expression is consequently reduced due to HPV 8 E7 expression. However, the expression levels of mature cytoplasmic 18S and 28S rRNA are retained. We propose that by relieving their resources from the energy-consuming process of rRNA transcription, HPV 8 E7 expressing cells might support more efficient virus replication in the differentiating epithelium.

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Acknowledgements

Funding for this work was provided by Canceropole du Grand-Est, France, Deutsches Krebsforschungszentrum, Heidelberg, Germany, and the Finnish Society of Sciences and Letters. Dr. Herbert Pfister (University of Cologne, Germany) is greatly acknowledged for the cloned sequences of HPV 5 and HPV 8.

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Authors and Affiliations

  1. Research Program in Infection and Cancer, Deutsches Krebsforschungszentrum, Heidelberg, Germany

    Evelyn Oswald, Eileen Reinz, Renate Voit & Angel Alonso

  2. Department of Dermatology, Université de Franche-Comté, Besançon, France

    François Aubin

  3. Medicum, Department of Virology, University of Helsinki and Helsinki University Hospital, POB 21, 00014, Helsinki, Finland

    Eeva Auvinen

Authors
  1. Evelyn Oswald
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  2. Eileen Reinz
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  3. Renate Voit
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  4. François Aubin
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  5. Angel Alonso
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  6. Eeva Auvinen
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Contributions

All authors have contributed to the design (EO, RV, FA, AA, EA), experimentation (EO, ER, FA), result interpretation (EO, ER, RV, FA, AA, EA) and/or writing (EO, ER, RV, FA, AA, EA) of this work.

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Correspondence to Eeva Auvinen.

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The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

The research presented here does not involve human participants of experimentation on animals.

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Informed consent is not required because no human participants were involved in the work.

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Edited by Hartmut Hengel.

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Oswald, E., Reinz, E., Voit, R. et al. Human papillomavirus type 8 E7 protein binds nuclear myosin 1c and downregulates the expression of pre-rRNA. Virus Genes 53, 807–813 (2017). https://doi.org/10.1007/s11262-017-1491-6

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  • DOI: https://doi.org/10.1007/s11262-017-1491-6

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