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Characterization and partial genomic analysis of a lytic Myoviridae bacteriophage against Staphylococcus aureus isolated from dairy cows with mastitis in Mid-east of China

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Abstract

Using bacteriophages as a tool to the control of pathogens is a complementary to antibiotic therapy. We have isolated a lytic bacteriophage, designated vB_SauM_JS25, from sewage effluent on a dairy farm in Jiangsu, Mid-east of China for use as a biocontrol agent against Staphylococcus aureus infections. Phage vB_SauM_JS25 was morphologically classified as Myoviridae. The phage showed broad host ranges within S. aureus strains, lysing 51 of 56 strains (91.1 %). Its latent period and burst size were approximately 20 min and 21 PFU/cell, respectively. Phage vB_SauM_JS25 was able to survive in a pH range between 6 and 9. However, a treatment of 70 or 80 °C for 10 min completely inactivated the phage. Moreover, morphologic analysis of vB_SauM_JS25 revealed that it was closely related to other Myoviridae phages infecting Staphylococcus species. The bacteriolytic activity of phage vB_SauM_JS25 at a multiplicity infection (MOI) 1 indicted its efficiency for reducing bacterial growth. These findings suggest that phage vB_SauM_JS25 could be considered a potential therapeutic or prophylactic candidate against S. aureus infection.

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Acknowledgments

This work was supported by the Joint innovation and research funding (BY2013075), the Natural Science Foundation of Jiangsu Province (BK20140458), and the Jiangsu Agricultural Science and Technology Foundation [cx(13)3086].

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Authors and Affiliations

  1. Key Open Laboratory of Edible Agricultural Products Safe Monitoring and Control, Ministry of Agriculture, Nanjing, 210014, People’s Republic of China

    Lili Zhang, Hongduo Bao, Chengrui Wei, Hui Zhang, Yan Zhou & Ran Wang

  2. Institute of Food Safety & Detection, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, People’s Republic of China

    Lili Zhang, Hongduo Bao, Chengrui Wei, Hui Zhang, Yan Zhou & Ran Wang

Authors
  1. Lili Zhang
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  2. Hongduo Bao
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  3. Chengrui Wei
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  4. Hui Zhang
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  5. Yan Zhou
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  6. Ran Wang
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Correspondence to Ran Wang.

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Zhang, L., Bao, H., Wei, C. et al. Characterization and partial genomic analysis of a lytic Myoviridae bacteriophage against Staphylococcus aureus isolated from dairy cows with mastitis in Mid-east of China. Virus Genes 50, 111–117 (2015). https://doi.org/10.1007/s11262-014-1130-4

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  • DOI: https://doi.org/10.1007/s11262-014-1130-4

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