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Host genotype overrides endophyte infection effects on growth, physiology, and nutrient content of a native grass, Achnatherum sibiricum

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Abstract

The effect of infection by the fungal endophyte Neotyphodium, host genotype, and their interaction on growth and physiology, as well as photosynthesis, was investigated in the native grass Achnatherum sibiricum. We artificially inoculated the endophyte into mature tillers of endophyte-free A. sibiricum. Plants were clipped to 5 cm height after recording growth traits, and analyzed for total nonstructural carbohydrates (TNC %), the percentage of nitrogen (N %), and carbon (C %) in leaves before and after clipping. In our study, the prominent host genotype–endophyte infection interactions detected in A. sibiricum indicates that, for many growth and storage traits, endophyte infection can impact a little change. However, there is no overriding consistently positive effect of the endophyte on growth or storage in A. sibiricum before or after clipping. Our study showed that the interaction between endophyte and host grasses was highly contingent on plant genotypes. We found host genotype overrode fungal endophyte infection in influencing tiller number and photosynthetic properties of A. sibiricum before clipping. After clipping, host genotype accounted for more of the variation in regrowth and above-ground biomass of A. sibiricum than endophyte infection. Our study showed that host genotype affected the total nonstructural carbohydrates of A. sibiricum before and after clipping, whereas endophyte infection increased the carbon content after clipping. Genotype by infection interactions for plant height, leaf mass, total nonstructural carbohydrates, and photosynthetic characteristics indicated genotype-specific effects of endophytes on A. sibiricum physiology and photosynthetic capacity. The host genotype–endophyte infection interactions detected in A. sibiricum suggest that host genotype overrides fungal endophyte infection on growth, physiology, and nutrient content of this native grass. In contrast, endophyte effects did not appear to positively affect growth, physiology, or photosynthetic capacity before or after clipping.

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Acknowledgments

This research was funded by the National Natural Science Foundation (31270463) and the Doctoral Program Foundation of Institutions of Higher Education of China (20130031110023).

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  1. College of Life Science, Nankai University, Tianjin, 300071, China

    Tong Jia, An-Zhi Ren & Yu-Bao Gao

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  1. Tong Jia
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Correspondence to Yu-Bao Gao.

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Communicated by Lori Biederman.

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Jia, T., Ren, AZ. & Gao, YB. Host genotype overrides endophyte infection effects on growth, physiology, and nutrient content of a native grass, Achnatherum sibiricum . Plant Ecol 215, 875–887 (2014). https://doi.org/10.1007/s11258-014-0339-6

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